A5086357892- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Pluripotent Stem Cells Research
- Chromosomal and Genetic Variations
- Reproductive System and Pregnancy
- Retinal Development and Disorders
- Gene expression and cancer classification
- Insect symbiosis and bacterial influences
- Endometriosis Research and Treatment
- Diverse Applied Research Studies
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Mosquito-borne diseases and control
- Reproductive Biology and Fertility
- Genetics, Aging, and Longevity in Model Organisms
- Knowledge Societies in the 21st Century
- RNA Research and Splicing
- Violence, Education, and Gender Studies
- Genomics and Chromatin Dynamics
- Microbial Natural Products and Biosynthesis
- Fungal and yeast genetics research
- Social Skills and Education
- RNA Interference and Gene Delivery
Rice University
2021-2025
Technological University of Pereira
2021
Brigham Young University - Idaho
2017-2019
Histone phosphorylation is a ubiquitous post-translational modification that allows eukaryotic cells to rapidly respond environmental stimuli. Despite correlative evidence linking histone changes in gene expression, establishing the causal role of this key epigenomic at diverse loci within native chromatin has been hampered by lack technologies enabling robust, locus-specific deposition endogenous phosphorylation. To address technological gap, here we build programmable kinase, called...
Protein intrinsically disordered regions (IDRs) are critical gene-regulatory components and aberrant fusions between IDRs DNA-binding/chromatin-associating domains cause diverse human cancers. Despite this importance, how influence gene expression, IDR fusion proteins provoke oncogenesis, remains incompletely understood. Here we develop a series of synthetic dCas9-IDR to establish that locus-specific recruitment can be sufficient stimulate endogenous expression. Using dCas9 fused the...
Nuclease-inactivated CRISPR/Cas-based (dCas-based) systems have emerged as powerful technologies to synthetically reshape the human epigenome and gene expression. Despite increasing adoption of these platforms, their relative potencies mechanistic differences are incompletely characterized, particularly at enhancer-promoter pairs. Here, we systematically compared most widely adopted dCas9-based transcriptional activators, well an activator consisting dCas9 fused catalytic core CBP protein,...
Abstract Engineered transactivation domains (TADs) combined with programmable DNA binding platforms have revolutionized synthetic transcriptional control. Despite recent progress in CRISPR–Cas-based (CRISPRa) technologies, the TADs used these systems often contain poorly tolerated elements and/or are prohibitively large for many applications. Here, we defined and optimized minimal built from human mechanosensitive transcription factors. We components to construct potent compact multipartite...
Genome mining of cryptic natural products (NPs) remains challenging, especially in filamentous fungi, owing to their complex genetic regulation. Increasing evidence indicates that several epigenetic modifications often act cooperatively control fungal gene transcription, yet the ability predictably manipulate multiple genes simultaneously is still largely limited. Here, we developed a multiplex base-editing (MBE) platform significantly improves capability and throughput genome manipulation,...
Nuclease-deactivated Cas (dCas) proteins can be used to recruit epigenetic effectors, and this class of editing technologies has revolutionized the ability synthetically control mammalian epigenome transcriptome. DNA methylation is one most important well-characterized modifications in mammals, while many different forms dCas-based methyltransferases (dCas-DNMTs) have been developed for programmable methylation, these tools are frequently poorly tolerated and/or lowly expressed cell types....
Engineering histone acylation states can inform mechanistic epigenetics and catalyze therapeutic epigenome editing opportunities. Here, we developed engineered lysine acyltransferases that enable the programmable deposition of acetylation longer-chain acylations. We show targeting an crotonyltransferase results in weak levels endogenous enhancer activation yet retains potency when targeted to promoters. further identify a single mutation within catalytic core human p300 preserves enzymatic...
Ribonucleic acid (RNA) is widely used in molecular biology assays, and some of the most common assays include: northern blotting RT-PCR gene expression analysis. RNA generally extracted by two methods: phenol-chloroform or commercially available silica spin column kits. Phenol-chloroform extraction more economical; however, it produces hazardous byproducts, leftover chemicals sample that can inhibit downstream applications. Commercial kits usually have simple set ups short preparation time;...
CRISPR/Cas technologies have revolutionized the ability to redesign genomic information and tailor endogenous gene expression. Nevertheless, discovery development of new systems has resulted in a lack clarity surrounding relative efficacies among these human cells. This deficit makes optimal selection cells unnecessarily challenging, which turn hampers their adoption, thus ultimately limits utility. Here, we designed series testbed methodically quantify compare genome editing, CRISPRi,...
Abstract Engineered transactivation domains (TADs) combined with programmable DNA binding platforms have revolutionized synthetic transcriptional control. Despite recent progress in CRISPR/Cas-based (CRISPRa) technologies, the TADs used these systems often contain poorly tolerated elements and/or are prohibitively large for many applications. Here we defined and optimized minimal built from human mechanosensitive transcription factors (MTFs). We components to construct potent compact...
Se realiza la propuesta de una técnica análisis datos cualitativos basados en recolección por medio un instrumento unidades del color RGB (Rojo, Verde y Azul) como fundamento el proceso estudio corpus textual concepto electromagnetismo estudiantes ingeniería física Universidad Tecnológica Pereira. Determinar eficacia método recopilación que conlleve a apropiación conocimiento motivo investigación ser afianzado. conto con 10 dieron respuesta formulario Google vez obtenida base datos, se...