A5074875737- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Pain Mechanisms and Treatments
- Receptor Mechanisms and Signaling
- Cancer, Stress, Anesthesia, and Immune Response
- Neuropeptides and Animal Physiology
- Pharmacological Receptor Mechanisms and Effects
- Memory and Neural Mechanisms
- Stress Responses and Cortisol
- Neural dynamics and brain function
- Pancreatic function and diabetes
- Genetics, Aging, and Longevity in Model Organisms
- Photoreceptor and optogenetics research
- Pain Management and Opioid Use
- Biochemical effects in animals
- Advanced Chemical Sensor Technologies
- Pain Management and Placebo Effect
- Infant Health and Development
- Neuroendocrine regulation and behavior
- Circadian rhythm and melatonin
University of Pennsylvania
2022-2024
Vanderbilt University
2016-2024
California University of Pennsylvania
2022-2023
Institute of Molecular Biology and Biophysics
2021
Allen Institute for Brain Science
2021
Addiction Research Foundation
2021
Vanderbilt University Medical Center
2020
John F. Kennedy Center for the Performing Arts
2019
Vanderbilt Health
2019
Excitatory signaling mediated by NMDARs has been shown to regulate mood disorders. However, current treatments targeting NMDAR subtypes have limited success in treating patients, highlighting a need for alternative therapeutic targets. Here, we identify role GluN2D-containing modulating emotional behaviors and neural activity the bed nucleus of stria terminalis (BNST). Using GluN2D KO mouse line (GluN2D −/− ), assessed behavioral phenotypes across tasks modeling behavior. We then used...
Active responses to stressors involve motor planning, execution, and feedback. Here we identify an insular cortex BNST (insula→BNST) circuit recruited during restraint stress-induced active struggling that modulates affective behavior. We demonstrate activity in this tightly follows behavioral events the size of fluorescent sensor transient reports duration struggle event, effect fades with repeated exposure homotypic stressor. Struggle are associated enhanced glutamatergic- decreased...
With concurrent global epidemics of chronic pain and opioid use disorders, there is a critical need to identify, target manipulate specific cell populations expressing the mu-opioid receptor (MOR). However, available tools transgenic models for gaining long-term genetic access MOR+ neural types circuits involved in modulating pain, analgesia addiction across species are limited. To address this, we developed catalog MOR promoter (MORp) based constructs packaged into adeno-associated viral...
The anterior cingulate cortex plays a pivotal role in the cognitive and affective aspects of pain perception. Both endogenous exogenous opioid signaling within mitigate cortical nociception, reducing unpleasantness. However, specific functional molecular identities cells mediating analgesia remain elusive. Given complexity as sensory emotional experience, richness ethological pain-related behaviors, we developed standardized, deep-learning platform for deconstructing behavior dynamics...
Relapse is a critical barrier to effective long-term treatment of alcoholism, and stress often cited as key trigger relapse. Numerous studies suggest that stress-induced reinstatement drug-seeking behaviors mediated by norepinephrine (NE) corticotropin-releasing factor (CRF) signaling interactions in the bed nucleus stria terminalis (BNST), brain region many behavioral physiologic responses stressors. Here, we sought directly examine effects NE on BNST CRF neuron activity determine whether...
The dorsal region of the bed nucleus stria terminalis (dBNST) receives substantial dopaminergic input which overlaps with norepinephrine implicated in stress responses. Using <i>ex vivo</i> fast scan cyclic voltammetry male C57BL6 mouse brain slices, we demonstrate that electrically stimulated dBNST catecholamine signals are substantially lower magnitude and have slower uptake rates compared caudate signals. Dopamine terminal autoreceptor activation inhibited roughly half transient,...
Key targets of both the therapeutic and abused properties opioids are μ-opioid receptors (MORs). Despite years research investigating biochemistry signal transduction pathways associated with MOR activation, we do not fully understand cellular mechanisms underlying opioid addiction. Given that addictive such as morphine, oxycodone, heroin, fentanyl all activate MORs, current therapies naloxone buprenorphine block this availability tools to mechanistically investigate opioid-mediated...
Alcohol use disorder (AUD) is a chronic, relapsing disease, highly comorbid with anxiety and depression. The bed nucleus of the stria terminalis (BNST),
Alcohol use disorder (AUD) is a chronic, relapsing disease, highly comorbid with anxiety and depression. The bed nucleus of the stria terminalis (BNST) Crh+ neurons in this region play key role chronic ethanol-induced increases volitional intake, hypothesized to be driven by emergent negative affective behaviors. Excitatory N-methyl-d-aspartate receptors (NMDARs) are major target ethanol, ethanol exposure has been shown regulate NMDAR function expression. Specifically, GluN2D...
<b>Abstract ID 16515</b> <b>Poster Board 143</b> Alcohol use disorder (AUD) is a chronic, relapsing disease, highly comorbid with anxiety and depression. These states of negative affect experienced during withdrawal are hypothesized to drive alcohol seeking relapse behavior. The bed nucleus the stria terminalis (BNST) brain region responsible for integration alcohol-related behaviors. Within this region, NMDARs major target ethanol, capable modulating ethanol-induced synaptic plasticity...