A5025687682- Neurotransmitter Receptor Influence on Behavior
- Neuroendocrine regulation and behavior
- Stress Responses and Cortisol
- Sleep and Wakefulness Research
- Neuroscience and Neuropharmacology Research
- Regulation of Appetite and Obesity
- Tryptophan and brain disorders
- Epigenetics and DNA Methylation
- Memory and Neural Mechanisms
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Circadian rhythm and melatonin
- Pain Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Prenatal Substance Exposure Effects
- Birth, Development, and Health
- RNA and protein synthesis mechanisms
- Cannabis and Cannabinoid Research
- Molecular Biology Techniques and Applications
- interferon and immune responses
- Sleep and related disorders
Temple University
2017-2024
University of Pennsylvania
2009-2022
Temple College
2021-2022
Sleep deprivation is a common problem of considerable health and economic impact in today's society. loss associated with deleterious effects on cognitive functions such as memory has high comorbidity many neurodegenerative neuropsychiatric disorders. Therefore, it crucial to understand the molecular basis effect sleep brain. In this study, we combined genome-wide traditional biological approaches determine cellular impacts mouse hippocampus, brain area for forms memory. Microarray analysis...
Sleep deprivation impairs memory formation by suppressing protein synthesis.
The sequencing of the full transcriptome (RNA-seq) has become preferred choice for measurement genome-wide gene expression. Despite its widespread use, challenges remain in RNA-seq data analysis. One often-overlooked aspect is normalization. fact that a variety factors or 'batch effects' can contribute unwanted variation to data, commonly used normalization methods only correct depth. study expression particularly problematic when it influenced simultaneously by biological addition one...
Abstract Endogenous homeostatic mechanisms can restore normal neuronal function following cocaine-induced neuroadaptations. Such may be exploited to develop novel therapies for cocaine addiction, but a molecular target has not yet been identified. Here we profiled mouse gene expression during early and late abstinence identify putative regulators of neural homeostasis. Cocaine activated the transcription factor, Nr4a1 , its gene, Cartpt key molecule involved in dopamine metabolism. Sustained...
Experiencing some early life adversity can have an "inoculating" effect that promotes resilience in adulthood. However, the mechanisms underlying stress inoculation are unknown, and animal models lacking. Here we used limited bedding nesting (LBN) model of to evaluate addiction-related phenotypes. In LBN, pups from postnatal days 2 9 their dams were exposed a low-resource environment. adulthood, they tested for addiction-like phenotypes compared rats raised standard housing conditions. High...
One of the most significant problems facing older individuals is difficulty staying asleep at night and awake during day. Understanding mechanisms by which regulation sleep/wake goes awry with age a critical step in identifying novel therapeutic strategies to improve quality life for elderly. We measured wake, non-rapid eye movement (NREM) rapid-eye (REM) sleep young (2–4 months-old) aged (22–24 C57BL6/NIA mice. used both conventional measures (i.e., bout number duration) an innovative...
A fundamental question in neuroscience is how memories are stored and retrieved the brain. Long-term memory formation requires transcription, translation epigenetic processes that control gene expression. Thus, characterizing genome-wide transcriptional changes occur after acquisition retrieval of broad interest importance. Genome-wide technologies commonly used to interrogate discovery-based approaches. Their ability increase scientific insight beyond traditional candidate approaches,...
We previously showed that paternal cocaine exposure reduced the reinforcing efficacy of in male offspring. Here, we sought to determine whether experience could also influence anxiety levels Male rats were allowed self-administer (controls received saline passively) for 60 days and then bred with naïve females. Measures cocaine-induced anxiogenic effects assessed adult Cocaine-sired offspring exhibited increased anxiety-like behaviors, as measured using novelty-induced hypophagia defensive...
Parental history of opioid exposure is seldom considered when prescribing opioids for pain relief. To explore whether parental may affect sensitivity to morphine in offspring, we developed a “rat scale” with high-speed imaging, machine learning, and mathematical modeling multigenerational model paternal self-administration. We find that the most commonly used tool measure mechanical rodents, von Frey hair, not painful rats during baseline conditions. also male progeny morphine-treated sires...
Astrocyte morphology affects function, including the regulation of glutamatergic signaling. This changes dynamically in response to environment. However, how early life manipulations alter adult cortical astrocyte is underexplored. Our lab uses brief postnatal resource scarcity, limited bedding and nesting (LBN) manipulation, rats. We previously found that LBN augments maternal behaviors promotes later resilience addiction-related behaviors, reducing impulsivity, risky decision-making,...
Gentle handling is commonly used to perform brief sleep deprivation in rodents. It was recently reported that daily acclimation handling, which often before behavioral assays, causes alterations sleep, stress, and levels of N-methyl-D-aspartate receptor subunits prior the actual period deprivation. therefore suggested could mediate some observed effects subsequent Here, we examine whether performed as our studies, alters sleep/wake behavior, or forms hippocampal synaptic plasticity are...