Abstract The RASSF1A tumor suppressor is involved in regulation of apoptosis and cell cycle progression. localized to microtubules binds the apoptotic kinases MST1 MST2. It has been shown that this interaction mediated by Sav‐RASSF‐Hpo domain, which an domain characterized for Drosophila proteins Sav (human WW45), Hpo MST2) Warts/LATS (large suppressor). Previously, we have reported hypermethylation occurs frequently soft tissue sarcoma associated with unfavorable prognosis cancer patients....

The Ras GTPases are a superfamily of molecular switches that regulate cellular proliferation and apoptosis in response to extra-cellular signals. regulation these pathways depends on the interaction with specific effectors. Recently, we have cloned characterized novel gene encoding putative effector: Ras-association domain family 1 (RASSF1) gene. RASSF1 is located chromosomal segment 3p21.3. high allelic loss variety cancers suggested crucial role this region tumorigenesis. At least two...

Epigenetic inactivation of the RASSF1A tumor suppressor by CpG island methylation was frequently detected in cancer. However, mechanisms this aberrant DNA are unknown. In promoter, we characterized four Sp1 sites, which methylated We examined functional relationship between methylation, histone modification, binding, and expression proliferating human mammary epithelial cells. With increasing passages, transcription dramatically silenced. This associated with deacetylation lysine 9...

Objective: To understand the role of epigenetic inactivation tumor-related genes in pathogenesis thyroid cancer, we investigated methylation profile distinct neoplasms. Design: We analyzed pattern 17 gene promoters nine cancer cell lines and 38 primary carcinomas (13 papillary carcinoma [PTC], 10 follicular [FTC], 9 undifferentiated [UTC], 6 medullary [MTC]), 12 goiters, adenomas (FA) by methylation- specific polymerase chain reaction (PCR). Epigenetic was validated expression analysis. Main...

Are ten-eleven-translocation (TET) 1-3 family enzymes involved in human spermatogenesis and do they impact male fertility?TET1, TET2 TET3 are successively expressed at different stages of spermatogenesis, their expression levels associate with fertility.Spermatogenesis is a complex cell differentiation process accompanied by drastic epigenetic remodeling. TET1-3 dioxygenases essential for active DNA demethylation the paternal pronucleus embryonic stem cells.Expression mRNAs proteinss...

Abstract Background The Ras association domain family (RASSF) encodes for distinct tumor suppressors and several members are frequently silenced in human cancer. In our study, we analyzed the role of RASSF2, RASSF3, RASSF4, RASSF5A, RASSF5C RASSF6 effectors MST1, MST2 WW45 thyroid carcinogenesis. Results Frequent methylation RASSF2 RASSF5A CpG island promoters tumors was observed. methylated 88% cancer cell lines 63% primary carcinomas. significantly increased carcinoma compared to normal...

Background: 16S rRNA analysis has been used in various diseases to identify pathogenic bacteria. In particular, pathogens that are difficult cultivate or previously unknown can be detected with great certainty. chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), a distinction between bacterial and non-bacterial genesis is essential regard categorization therapy. The objective of this study investigate the value gene sequence routine management patients CP/CPPS especially after...

To investigate intra-tumoural coexistence and heterogeneity of aberrant promoter hypermethylation different tumour suppressor genes in melanoma, we analyzed the distribution methylation RASSF1A, p16, DAPK, MGMT, Rb 339 assays 34 tumours (15 melanoma primaries, 19 metastases) by methylation-specific PCR, correlation to histopathology RASSF1A expression. We detected at least one gene 74% (30%, 52%, 33%, 20%, 40% for MGMT Rb, respectively). 70% cases exhibited an inhomogeneous pattern (17%,...

The Ras association domain family (RASSF) encodes for distinct tumor suppressors and several members are frequently silenced in human cancer. In our study, we analyzed the role of a novel RASSF member termed RASSF10 thyroid carcinogenesis. CpG island promoter was intensively methylated nine cancer cell lines 66% primary carcinomas. methylation significantly increased carcinoma compared to normal follicular adenoma (0% 10%, respectively; p

Are unexplained recurrent miscarriages associated with abnormal protamine-1 and protamine-2 mRNA levels in spermatozoa?Both as well the to ratio spermatozoa from men whose female partners experienced two or more consecutive were significantly different compared those both healthy control subfertile couples undergoing IVF/ICSI.Aberrant sperm protamine ratios are known be male-factor infertility. Data this study suggest that may additionally affect early embryo development.The population was...

Background: Chronic Prostatitis/Chronic Pelvic Pain Syndrome (CP/CPPS) is a frequent disease affecting men of every age and accounting for great number consultations at urology departments. Previous studies suggested negative impact CP/CPPS on fertility. As increasing attention has been attributed to additional aspects, such as sperm DNA integrity protein alterations, besides the WHO standard semen analysis when assessing male fertility, in this prospective study, we aimed further...

Abstract BACKGROUND Ras association domain family (RASSF) comprises several tumor suppressor genes, which are often epigenetically inactivated in human tumors. Here, we aim to analyze the relevance of recently identified member RASSF10 prostate carcinogenesis. METHODS promoter methylation and mRNA expression were investigated by bisulfite‐pyrosequencing qRT‐PCR, respectively, carcinoma (PCa) cell lines (LNCaP, 22Rv1, DU‐145) 83 primary PCa 53 benign prostatic hyperplasia (BPH) tissues...

Background/Aims/Objectives: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) has detrimental effects on the quality of life including aspect sexual dysfunction. The aim study was to identify if there an adverse effect male genital compartment and are systemic or compartment-specific local signals for epigenetic dysregulation inflammatory factors in CP/CPPS patients.One hundred five NIH IIIb patients 41 healthy men were recruited underwent investigations urines, semen blood....

The use of immune adjuvants such as toll-like receptor (TLR) agonists reflects a novel strategy in prostate cancer (PCa) therapy. However, interleukin-1 associated kinase 1 (IRAK1), central effector TLR signaling, has been shown to be responsible for resistance radiation-induced tumor cell death. In order better understand the function and epigenetic regulation IRAK1 PCa, we performed vitro culture experiments together with integrative bioinformatic studies using latest single-cell...