A5077969143- Sulfur Compounds in Biology
- Acute Myeloid Leukemia Research
- Cancer, Hypoxia, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Nitric Oxide and Endothelin Effects
- Telomeres, Telomerase, and Senescence
- Cancer Genomics and Diagnostics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Sarcoma Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Cardiovascular Effects of Exercise
- Kruppel-like factors research
- Fatty Acid Research and Health
- Multiple Myeloma Research and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Renin-Angiotensin System Studies
- Muscle Physiology and Disorders
- Advanced Nanomaterials in Catalysis
- 14-3-3 protein interactions
- Cardiomyopathy and Myosin Studies
- Folate and B Vitamins Research
- Single-cell and spatial transcriptomics
- Blood Coagulation and Thrombosis Mechanisms
- Erythrocyte Function and Pathophysiology
- Retinopathy of Prematurity Studies
Goethe University Frankfurt
2018-2023
German Centre for Cardiovascular Research
2018-2020
University Medical Centre Mannheim
2014-2018
Heidelberg University
2014-2018
Palmetto Hematology Oncology
2016
University Hospital Heidelberg
2014
Abstract Endothelial cells play a critical role in the adaptation of tissues to injury. Tissue ischemia induced by infarction leads profound changes endothelial cell functions and can induce transition mesenchymal state. Here we explore kinetics individual cellular responses after myocardial using single RNA sequencing. This study demonstrates time dependent switch proliferation inflammation associated with transient metabolic gene signatures. Trajectory analysis reveals that majority 3 7...
Hydrogen sulfide (H2S), generated by cystathionine γ lyase (CSE), is an important endogenous regulator of vascular function. The aim the present study was to investigate control and consequences CSE activity in endothelial cells under physiological proatherogenic conditions.Endothelial cell knockout mice were generated, lung studied vitro (gene expression, protein sulfhydration, monocyte adhesion). Mice crossed onto apolipoprotein E-deficient background, atherogenesis (partial carotid artery...
In vascular endothelial cells, cysteine metabolism by the cystathionine γ lyase (CSE), generates hydrogen sulfide-related sulfane sulfur compounds (H2Sn), that exert their biological actions via S-sulfhydration of target proteins. This study set out to map "S-sulfhydrome" (ie, spectrum proteins targeted H2Sn) in human cells.Liquid chromatography with tandem mass spectrometry was used identify S-sulfhydrated cysteines cell and β3 integrin intraprotein disulfide bond rearrangement. Functional...
Hydrogen sulfide (H2S) is a gasotransmitter that regulates cellular homeostasis and impacts on multiple physiological pathophysiological processes. However, it exerts many of its biological actions indirectly via the formation H2S-derived sulfane sulfur species/polysulfides. Because high reactivity species, detection polysulfides in systems challenging currently used methods are neither sensitive nor quantitative. Herein, we describe LC-MS/MS-based method makes use Sulfane Sulfur Probe 4 to...
Background: Limb-Girdle muscular dystrophies (LGMD) are a heritable group of genetically determined disorders with primary involvement the pelvic or shoulder girdle musculature partially cardiac manifestation, such as dilated cardiomyopathy (DCM) and life-threatening tachyarrhythmia. We report here that human induced pluripotent stem cell (hiPSC)–derived cardiomyocytes from patient LGMD2I DCM associated recurrent ventricular tachycardia displayed ion channel dysfunction abnormality calcium...
Cystathionine γ lyase (CSE) is the major source of hydrogen sulfide-derived species (H2Sn) in endothelial cells and plays an important role protecting against atherosclerosis. Here we investigated molecular mechanisms underlying regulation CSE expression by fluid shear stress/flow. Fluid stress decreased human murine was negatively correlated with transcription factor Krüppel-like (KLF) 2. identified as a direct target KLF2-regulated microRNA, miR-27b high native plaque-derived cells, also...
BACKGROUND: Advanced age is unequivocally linked to the development of cardiovascular disease; however, mechanisms resulting in reduced endothelial cell regeneration remain poorly understood. Here, we investigated novel involved senescence that impact transcription and vascular repair after injury. METHODS: Native cells were isolated from young (20±3.4 years) aged (80±2.3 individuals subjected molecular analyses assess global transcriptional metabolic changes. In vitro studies conducted...
Polyunsaturated fatty acids such as docosahexaenoic acid (DHA) positively affect the outcome of retinopathy prematurity (ROP). Given that DHA metabolism by cytochrome P450 and soluble epoxide hydrolase (sEH) enzymes affects retinal angiogenesis vascular stability, we investigated role sEH in a mouse model ROP. In WT mice, hyperoxia elicited tyrosine nitration inhibition decreased generation DHA-derived diol 19,20-dihydroxydocosapentaenoic (19,20-DHDP). Correspondingly, murine ROP, sEH-/-...
Tissue factor pathway inhibitor (TFPI) is an important regulator of coagulation and a link between inflammation thrombosis. Here we investigated whether endothelial cell-driven oxidative post-translational modifications could have impact on TFPI activity. We focused S-sulfhydration, which hydrogen sulfide-dependent modification that, in cells, regulated by the enzyme cystathionine γ-lyase (CSE). The study made use human primary cells blood from healthy individuals or subjects with...
Abstract Aims Advanced age is unequivocally linked to the development of cardiovascular disease, however, mechanisms leading loss endothelial cell regenerative capacity during aging remain poorly understood. Here we aimed investigate novel involved in senescence, that impact on transcription and vascular repair response upon injury Methods results RNA sequencing a unique collection native cells from young aged individuals, showed (20 vs. 80 years) characterized by p53- mediated reprogramming...
Retinopathy of prematurity (ROP) is a leading cause childhood blindness. Several signalling pathways have been implicated in the process ROP but novel drugable target cytochrome P450 (CYP)/soluble epoxide hydrolase (sEH) pathway. The latter enzymes play crucial role biological processes by generating bioactive epoxides and diols from endogenous polyunsaturated fatty acids (PUFAs). This study aimed to characterize effects newly identified sEH‐derived w‐3 PUFA diol;...