A5054694151- Hepatitis Viruses Studies and Epidemiology
- Viral gastroenteritis research and epidemiology
- Heat shock proteins research
- Connexins and lens biology
- SARS-CoV-2 and COVID-19 Research
- Liver Disease and Transplantation
- Hepatitis C virus research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- COVID-19 Impact on Reproduction
- Sepsis Diagnosis and Treatment
- Immune Response and Inflammation
- Nicotinic Acetylcholine Receptors Study
- Immune cells in cancer
- Yersinia bacterium, plague, ectoparasites research
- Pneumonia and Respiratory Infections
- Animal Virus Infections Studies
- Influenza Virus Research Studies
- Vitamin C and Antioxidants Research
- Endoplasmic Reticulum Stress and Disease
- Nitric Oxide and Endothelin Effects
- Phagocytosis and Immune Regulation
- Liver Disease Diagnosis and Treatment
- Ion channel regulation and function
- Hepatitis B Virus Studies
University Hospital Heidelberg
2023-2025
Heidelberg University
2021-2025
Center for Experimental and Clinical Infection Research
2022-2023
Helmholtz Centre for Infection Research
2022-2023
Yale University
2020
Pulmonary and Critical Care Associates
2020
Leibniz University Hannover
2018-2019
Introduction Procalcitonin expression is thought to be stimulated by bacteria and suppressed viruses via interferon signalling. Consequently, during respiratory viral illness, clinicians often interpret elevated procalcitonin as evidence of bacterial coinfection, prompting antibiotic administration. We sought evaluate the validity this practice underlying assumption that infection inhibits synthesis. Methods conducted a retrospective cohort study patients hospitalised with pure (n=2075)...
Double membrane vesicles (DMVs) serve as replication organelles of plus-strand RNA viruses such hepatitis C virus (HCV) and SARS-CoV-2. Viral DMVs are morphologically analogous to formed during autophagy, but lipids driving their biogenesis largely unknown. Here we show that production the lipid phosphatidic acid (PA) by acylglycerolphosphate acyltransferase (AGPAT) 1 2 in ER is important for DMV viral autophagy. Using HCV-replicating cells model, found AGPATs recruited critically contribute...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) remodels the endoplasmic reticulum (ER) to form replication organelles, leading ER stress and unfolded protein response (UPR). However, role of specific UPR pathways in infection remains unclear. Here, we found that SARS-CoV-2 causes marginal activation signaling sensor IRE1α its phosphorylation, clustering dense ER-membrane rearrangements with embedded membrane openings, XBP1 splicing. By investigating factors regulated by...
Hepatitis E virus (HEV) is the most common cause of acute viral hepatitis worldwide and mainly transmitted via fecal-oral route or through consumption contaminated food products. Due to lack efficient cell culture systems for propagation HEV, limited data regarding its sensitivity chemical disinfectants are available. Consequently, preventive evidence-based hygienic guidelines on HEV disinfection lacking.We used a robust genotype 3 model which enables quantification infection quasi-enveloped...
Hepatitis E virus (HEV) is the leading cause of acute viral hepatitis. Numerous studies have investigated dynamics HEV infection markers, but most suitable marker for diagnosing ongoing or recent remains to be determined. Recent evidence suggests that serum antigen testing superior IgM and RNA quantification. Moreover, it has been found infected individuals excrete in significant quantities through urine. To address this question, we conducted a longitudinal analysis involving 16 patients...
Gap junction channels and hemichannels formed by concatenated connexins were analyzed. Monomeric (hCx26, hCx46), homodimeric (hCx46-hCx46, hCx26-hCx26), heterodimeric (hCx26-hCx46, hCx46-hCx26) constructs, coupled to GFP, expressed in HeLa cells. Confocal microscopy showed that the tandems gap plaques with a reduced plaque area compared monomeric hCx26 or hCx46. Dye transfer experiments concatenation allows metabolic transfer. Expressed Xenopus oocytes, inside-out patch-clamp configuration...
Connexins (Cx) are proteins that form cell‐to‐cell gap junction channels. A mutation at position 188 in the second extracellular loop (E2) domain of hCx46 has been linked to an autosomal dominant zonular pulverulent cataract. As it is dominantly inherited, possible mutant variant affects co‐expressed wild‐type Cx and/or its interaction with other cellular components. Here, we proposed use concatenated hC x46wt‐ x46N188T and x46N188T‐ x46wt analyze how affected achieve a inheritance....
The past two decades have witnessed a resurgence in neutrophil research, inspired part by the discovery of extracellular traps (NETs) and their myriad roles health disease. Within lung, dysregulation neutrophils NETosis been linked to an array diseases including pneumonia, cystic fibrosis, acute respiratory distress syndrome (ARDS), chronic obstructive pulmonary disease (COPD), severe asthma. However, our understanding pathologic responses lung remains incomplete. Two methodologic issues...