A5032860993- SARS-CoV-2 and COVID-19 Research
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Liver Disease Diagnosis and Treatment
- Viral gastroenteritis research and epidemiology
- Viral Infections and Outbreaks Research
- Virus-based gene therapy research
- Mosquito-borne diseases and control
- Regulation of Appetite and Obesity
- Pharmacological Receptor Mechanisms and Effects
- Graphene and Nanomaterials Applications
- CRISPR and Genetic Engineering
- Essential Oils and Antimicrobial Activity
- interferon and immune responses
- HIV Research and Treatment
- SARS-CoV-2 detection and testing
- Autophagy in Disease and Therapy
- Carbon and Quantum Dots Applications
- Diabetes and associated disorders
- Monoclonal and Polyclonal Antibodies Research
- Computational Drug Discovery Methods
- Viral Infections and Immunology Research
- Cellular transport and secretion
Institut Pasteur de Lille
2016-2025
Université de Lille
2016-2025
Inserm
2016-2025
Centre Hospitalier Universitaire de Lille
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Center for Infection and Immunity of Lille
2016-2025
Hôpital Raymond-Poincaré
2021
Assistance Publique – Hôpitaux de Paris
2021
Université Lille Nord de France
2011-2020
Institut Pasteur
2005-2020
The coronavirus spike protein (S) plays a key role in the early steps of viral infection, with S1 domain responsible for receptor binding and S2 mediating membrane fusion. In some cases, S is proteolytically cleaved at S1-S2 boundary. case severe acute respiratory syndrome (SARS-CoV), it has been shown that virus entry requires endosomal protease cathepsin L; however, was also found infection SARS-CoV could be strongly induced by trypsin treatment. Overall, terms how cleavage might activate...
Due to difficulties in cell culture propagation, the mechanisms of hepatitis C virus (HCV) entry are poorly understood. Here, postbinding cellular HCV were studied using both retroviral particles pseudotyped with envelope glycoproteins (HCVpp) and clone JFH-1 propagated (HCVcc). HCVpp was measured by quantitative real-time PCR after 3 h contact target cells, HCVcc infection quantified immunoblot analysis immunofluorescence detection proteins expressed infected cells. The functional role...
Many viral fusion proteins are primed by proteolytic cleavage near their peptides. While the coronavirus (CoV) spike (S) protein is known to be cleaved at S1/S2 boundary, this site not closely linked a peptide. However, second has been identified in severe acute respiratory syndrome CoV (SARS-CoV) S2 domain (R797). Here, we investigated whether internal of exposes We show that residues immediately C-terminal SARS-CoV SFIEDLLFNKVTLADAGF very highly conserved across all CoVs. Mutagenesis...
Therapeutic options for the highly pathogenic human coronavirus (HCoV) infections are urgently needed. Anticoronavirus therapy is however challenging, as coronaviruses biologically diverse and rapidly mutating. In this work, antiviral activity of seven different carbon quantum dots (CQDs) treatment HCoV-229E was investigated. The first generation CQDs derived from hydrothermal carbonization ethylenediamine/citric acid precursors postmodified with boronic ligands. These nanostructures showed...
Here, we identify (−)-epigallocatechin - 3 -gallate (EGCG) as a new inhibitor of hepatitis C virus (HCV) entry. EGCG is flavonoid present in green tea extract belonging to the subclass catechins, which has many properties. Particularly, possesses antiviral activity and impairs cellular lipid metabolism. Because close links between HCV life cycle metabolism, postulated that may interfere with infection. We demonstrate concentration 50 μM inhibits infectivity by more than 90% at an early step...
A better understanding of the anti-SARS-CoV-2 immune response is necessary to finely evaluate commercial serological assays but also predict protection against reinfection and help development vaccines. For this reason, we monitored antibody in infected patients. In order assess time seroconversion, used 151 samples from 30 COVID-19 inpatients detection kinetics anti-S1, anti-S2, anti-RBD anti-N antibodies with in-house ELISAs. We observed that specific were detectable all two weeks...
ABSTRACT Due to the recent development of a cell culture model, hepatitis C virus (HCV) can be efficiently propagated in culture. This allowed us reinvestigate subcellular localization HCV structural proteins context an infectious cycle. In agreement with previous reports, confocal immunofluorescence analysis indicated that, infected cells, glycoprotein heterodimer is retained endoplasmic reticulum. However, contrast other studies, did not accumulate intracellular compartments or at plasma...
Abstract Hepatitis C virus (HCV) particles are known to be in complex with lipoproteins. As a result of this interaction, the low-density lipoprotein (LDL) receptor (LDLR) has been proposed as potential entry factor for HCV; however, its implication remains unclear. Here, we reinvestigated role LDLR HCV life cycle by comparing mechanism uptake. A small interfering RNA targeting Huh-7 cells reduced infectivity, confirming that plays generated cell culture. However, kinetics internalization...
Hepatitis C virus (HCV)-infected patients undergoing liver transplantation universally experience rapid reinfection of their new graft. Current treatment protocols do not prevent graft and, in addition, an accelerated disease progression is observed. In the present study, we have evaluated a novel strategy to HCV infection using lectin, griffithsin (GRFT) that specifically binds N-linked high-mannose oligosaccharides are on viral envelope. The antiviral effect GRFT was vitro pseudoparticle...
Despite the validation of direct-acting antivirals for hepatitis C treatment, discovery new compounds with different modes action may still be importance treatment special patient populations. We recently identified a natural molecule, epigallocatechin-3-gallate (EGCG), as an inhibitor virus (HCV) targeting viral particle. The aim this work was to discover higher anti-HCV activity than that EGCG and determine their mode action. Eight molecules structure similarity were selected. HCV JFH1 in...
Mounting evidence suggests that the gut-to-lung axis is critical during respiratory viral infections. We herein hypothesized disruption of gut homeostasis severe acute syndrome coronavirus 2 (SARS-CoV-2) infection may associate with early disease outcomes. To address this question, we took advantage Syrian hamster model. Our data confirmed model recapitulates some hallmark features human in lungs. further showed SARS-CoV-2 associated mild intestinal inflammation, relative alteration barrier...
Many studies on SARS-CoV-2 have been performed over short-time scale, but few focused the ultrastructural characteristics of infected cells. We used TEM to perform kinetic analysis ultrastructure SARS-CoV-2-infected Early infection events were characterized by presence clusters single-membrane vesicles and stacks membrane containing nuclear pores called annulate lamellae (AL). A large network host cell-derived organelles transformed into virus factories was subsequently observed in As...
Efforts to mitigate the coronavirus disease 2019 (COVID-19) pandemic include screening of existing antiviral molecules that could be repurposed treat severe acute respiratory syndrome 2 (SARS-CoV-2) infections. Although SARS-CoV-2 replicates and propagates efficiently in African green monkey kidney (Vero) cells, antivirals such as nucleos(t)ide analogs (nuc’s) often show decreased activity these cells due inefficient metabolization.
SARS-CoV-2, although not being a circulatory virus, spread from the respiratory tract resulting in multiorgan failures and thrombotic complications, hallmarks of fatal COVID-19. A convergent contributor could be platelets that beyond hemostatic functions can carry infectious viruses. Here, we profiled 52 patients with severe COVID-19 demonstrated circulating 19 out 20 non-survivor contain SARS-CoV-2 robust correlation outcome. Platelets containing might originate bone marrow lung...
SARS-CoV-2 spike (S) envelope glycoprotein constitutes the main determinant of virus entry and target host immune response, thus being great interest for antiviral research. It is constituted S1 S2 subunits, which are involved in ACE2 receptor binding fusion between viral cell membrane, respectively. Induction process requires S cleavage at S1-S2 junction S2' site located upstream peptide. Interestingly, harbors a 4-residue insertion that absent its closest relatives polybasic motif...
Abstract Older age is one of the strongest risk factors for severe COVID-19. In this study, we determined whether age-associated cellular senescence contributes to severity experimental Aged golden hamsters accumulate senescent cells in lungs, and senolytic drug ABT-263, a BCL-2 inhibitor, depletes these at baseline during SARS-CoV-2 infection. Relative young hamsters, aged had greater viral load acute phase infection displayed higher levels sequelae post-acute phase. Early treatment with...
Obesity is a major public health problem and often associated with type 2 diabetes mellitus, cardiovascular disease, metabolic syndrome. Leptin the crucial adipostatic hormone that controls food intake body weight through activation of specific leptin receptors (OB-R) in hypothalamic arcuate nucleus (ARC). However, most obese patients, high circulating levels fail to bring about loss. The prevention this "leptin resistance" goal for obesity research. We report here successful diet-induced...
Hepatitis C virus (HCV) infections most often result in chronic outcomes, although the constantly produces replication intermediates, particular double-stranded RNA (dsRNA), representing potent inducers of innate immunity. We aimed to characterize fate HCV dsRNA hepatocyte cultures identify mechanisms contributing viral persistence presence an active immune response.We analyzed hepatocyte-based culture models for induction immunity, secretion positive- or negative-strand RNA, and using...
Hepatitis C virus (HCV) entry involves binding to cell surface heparan sulfate (HS) structures. However, due the lipoprotein-like structure of HCV, exact contribution virion components this interaction remains controversial. Here, we investigated relative HCV envelope proteins and apolipoprotein E in HS-binding step. Deletion hypervariable region 1, a previously proposed be involved HS binding, did not alter HS, indicating that is context viral infection. Patient sera monoclonal antibodies...