A5027174414- Microbial Natural Products and Biosynthesis
- Hepatitis Viruses Studies and Epidemiology
- Viral gastroenteritis research and epidemiology
- Marine Sponges and Natural Products
- Hepatitis C virus research
- Liver Disease and Transplantation
- Fungal Biology and Applications
- Medicinal Plant Research
- Genomics and Phylogenetic Studies
- Echinoderm biology and ecology
- Alkaloids: synthesis and pharmacology
- Hepatitis B Virus Studies
- Essential Oils and Antimicrobial Activity
- Phytochemistry and Bioactivity Studies
- Seaweed-derived Bioactive Compounds
- Research on Leishmaniasis Studies
- Plant Pathogens and Fungal Diseases
- Chemical synthesis and alkaloids
- Carbohydrate Chemistry and Synthesis
- Plant biochemistry and biosynthesis
- Mycorrhizal Fungi and Plant Interactions
- Curcumin's Biomedical Applications
- Biochemical and Structural Characterization
- Dengue and Mosquito Control Research
- Plant Growth and Agriculture Techniques
Center for Experimental and Clinical Infection Research
2018-2022
Ruhr University Bochum
2019-2022
National Research and Innovation Agency
2021-2022
National Nuclear Energy Agency of Indonesia
2022
Indonesian Institute of Sciences
2018-2022
Helmholtz Centre for Infection Research
2018-2020
Hepatitis E virus (HEV) is the causative agent of hepatitis in humans and leading cause for acute viral worldwide. The classified as a member genus Orthohepevirus A within Hepeviridae family. Due to absence robust cell culture model HEV infection, analysis life cycle, development effective antivirals vaccine severely limited. In this study, we established protocol based on genotype 3 p6 (Kernow C-1) human hepatoma lines HepG2 HepG2/C3A with different media conditions produce intracellular...
Hepatitis E virus (HEV) is the causative agent of hepatitis in humans and a member genus Orthohepevirus family Hepeviridae. HEV infections are common cause acute but can also take chronic courses. Ribavirin treatment choice for most patients type I interferon (IFN) has been evaluated few infected transplantation vivo. However, no effective specific treatments against currently available. In this study, we natural compound silvestrol, isolated from plant Aglaia foveolata, known its inhibition...
Rhodatin (1), a meroterpenoid featuring unique pentacyclic scaffold with both spiro and spiroketal centers, five unusual acorane-type sesquiterpenoids, named rhodocoranes A–E (2–6, respectively), are the first natural products isolated from basidiomycete Rhodotus palmatus. Their structures were elucidated by two-dimensional NMR experiments HRESIMS, while absolute configuration of substance family was determined Mosher's method utilizing 2. strongly inhibited hepatitis C virus, whereas 4...
Hepatitis E virus (HEV) is the most common cause of acute viral hepatitis worldwide and mainly transmitted via fecal-oral route or through consumption contaminated food products. Due to lack efficient cell culture systems for propagation HEV, limited data regarding its sensitivity chemical disinfectants are available. Consequently, preventive evidence-based hygienic guidelines on HEV disinfection lacking.We used a robust genotype 3 model which enables quantification infection quasi-enveloped...
The manuscript investigated the isolation, characterization and anti-infective potential of valinomycin (3), streptodepsipeptide P11A (2), P11B (1), one novel analogue, SV21 (4), which were all produced by Gram-positive strain Streptomycescavourensis SV 21. Although exact molecular weight major fragments recently reported for compound 4, its structure elucidation was not based on isolation spectroscopic techniques. We successfully isolated elucidated MS2 fragmentation pathways as well 1H 13C...
4-Hydroxypleurogrisein, a congener of the anticancer-lead compound pleurotin, as well six further derivatives were isolated from basidiomycete Hohenbuehelia grisea, strain MFLUCC 12-0451. The structures elucidated utilizing high resolution electron spray ionization mass spectrometry (HRESIMS) and 1D 2D nuclear magnetic resonance (NMR) spectral data evaluated for their biological activities; leucopleurotin, we provide Xray data. While most congeners showed moderate antimicrobial cytotoxic...
Two new secondary metabolites, labindole A [2-methyl-3-(2-nitroethyl)-3H-indole] (1) and B [2-methyl-3-(2-nitrovinyl)-3H-indole] (2), were isolated from the myxobacterium Labilithrix luteola (DSM 27648T). Additionally, four metabolites 3, 4, 5 6 already known other sources obtained. Their structures elucidated high resolution electrospray ionisation mass spectrometry (HRESIMS) 1D 2D nuclear magnetic resonance (NMR) spectroscopy data their relative configuration was assigned based on...
In order to minimize re-discovery of already known anti-infective compounds, we focused our screening approach on understudied, almost untapped marine environments including invertebrates and their associated bacteria. Therefore, two sea cucumber species, Holothuria leucospilota Stichopus vastus, were collected from Lampung (Indonesia), 127 bacterial strains identified by partial 16S rRNA-gene sequencing analysis compared with the NCBI database. addition, overall diversity tissue samples...
Hepatitis E virus (HEV) infections are a leading cause of acute viral hepatitis in humans and pose considerable threat to public health. Current standard care treatment is limited the off-label use nucleoside-analog ribavirin (RBV) PEGylated interferon-α, both which associated with significant side effects provide efficacy. In past few years, promising natural product compound class eukaryotic initiation factor 4A (eIF4A) inhibitors (translation inhibitors), called rocaglates, were...
Fermentation of the fungal strain Skeletocutis sp. originating from Mount Elgon Natural Reserve in Kenya, followed by bioassay guided fractionation led to isolation 12 previously undescribed metabolites named skeletocutins A-L (1-5 and 7-13) together with known tyromycin A (6). Their structures were assigned NMR spectroscopy complemented HR-ESIMS. Compounds 1-6 11-13 exhibited selective activities against Gram-positive bacteria, while compound 10 weakly inhibited formation biofilm...
Lanyamycin ( 1 / 2 ), a secondary metabolite occurring as two epimers, was isolated from the myxobacterium Sorangium cellulosum , strain Soce 481. The structures of both epimers were elucidated HRESIMS and 1D 2D NMR data relative configuration their macrolactone ring assigned based on NOE vicinal H coupling constants by calculation 3D model. inhibited HCV infection into mammalian liver cells with an IC 50 value 11.8 µM, exhibited moderate cytotoxic activity against mouse fibroblast cell line...
Hepatitis E virus (HEV) is an increasingly recognised pathogen, affecting several hundred thousand individuals in western countries each year. Importantly, the majority of immunocompromised are not able to clear HEV but develop a chronic course infection. In case lymphoma, which inherent immunosuppressive disease per se, chemotherapy can even further exacerbate status. As mechanism chronification barely understood, it important gain knowledge about influence chemotherapeutic drugs on...
Background and Objectives As previous investigations have shown, THERAFLEX UV‐Platelets, a UVC‐based pathogen inactivation (PI) system, is effective against non‐enveloped transfusion‐relevant viruses such as hepatitis A virus (HAV), which are insensitive to most PI treatments for blood products. This study investigated the efficacy of UV‐Platelets HEV in platelet concentrates (PCs). Materials Methods Buffy coat‐derived PCs additive solution were spiked with cell culture‐derived treated...
Two known Polybrominated Diphenyl Ethers (PBDEs), 3,4,5-tribromo-2-(2',4'-dibromophenoxy)phenol (1d) and 3,4,5,6-tetrabromo-2-(2',4'-dibromophenoxy)phenol (2b), were isolated from the Indonesian marine sponge Lamellodysidea herbacea. The structure was confirmed using 13C chemical shift average deviation compared to predicted structures recorded shifts in previous studies. We found a wide range of bioactivities organic crude extract, such as (1) strong deterrence against generalist pufferfish...
Abstract Exploration of secondary metabolites secreted by new Actinobacteria taxa isolated from unexplored areas, can increase the possibility to obtain compounds which be developed into drugs for treatment serious diseases such as hepatitis C. In this context, one actinobacterial strain, CG3, has been selected based on results polyphasic characterization, indicate that it represents a putative species within genus Nocardiopsis . Two fractions (F2 and F3), prepared culture strain CG3 in...