A5080196498- Nanoplatforms for cancer theranostics
- Connexins and lens biology
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Retinal Development and Disorders
- RNA and protein synthesis mechanisms
- Glioma Diagnosis and Treatment
- RNA modifications and cancer
- Autophagy in Disease and Therapy
- Signaling Pathways in Disease
- Magnesium in Health and Disease
- Caveolin-1 and cellular processes
- Biochemical effects in animals
- Eosinophilic Esophagitis
- Advanced Fluorescence Microscopy Techniques
- Gene expression and cancer classification
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- IL-33, ST2, and ILC Pathways
- Parathyroid Disorders and Treatments
- Trace Elements in Health
- Photoacoustic and Ultrasonic Imaging
- FOXO transcription factor regulation
Suzhou Institute of Biomedical Engineering and Technology
2015-2025
Chinese Academy of Sciences
2018-2025
University of Science and Technology of China
2020-2025
Nanjing University of Chinese Medicine
2024
State Key Laboratory of Genetic Engineering
2020-2021
Fudan University
2020-2021
Helmholtz Zentrum München
2009-2020
South Dakota State University
2019
Friedrich Schiller University Jena
2017
Jena University Hospital
2017
Abstract Aberrant N6-methyladenosine (m6A) modification has emerged as a driver of tumor initiation and progression, yet how long noncoding RNAs (lncRNA) are involved in the regulation m6A remains unknown. Here we utilize data from 12 cancer types The Cancer Genome Atlas to comprehensively map lncRNAs that potentially deregulated by DNA methylation. A novel methylation–deregulated RNA reader–cooperating lncRNA (DMDRMR) facilitated growth metastasis clear cell renal carcinoma (ccRCC)....
Cholesterol increases the risk of aggressive prostate cancer and has emerged as a potential therapeutic target for cancer. The functional roles cholesterol in metastasis are not fully understood. Here, we found that induces epithelial-to-mesenchymal transition (EMT) through extracellular-regulated protein kinases 1/2 pathway activation, which is mediated by EGFR adipocyte plasma membrane-associated (APMAP) accumulation cholesterol-induced lipid rafts. Mechanistically, APMAP interaction with...
Crystal structures of the CorC Mg 2+ transporter revealed unique binding site associated with genetic diseases.
Cytochrome c oxidase (COX) is the terminal enzyme of mitochondrial electron transport chain. The purpose this study was to analyze function lung-specific cytochrome subunit 4 isoform 2 (COX4i2) in vitro and COX4i2-knockout mice vivo. COX isolated from cow lung liver as control functionally analyzed. were generated effect gene knockout determined, including activity, tissue energy levels, noninvasive invasive function, pathology. These studies complemented by a comprehensive functional screen...
Cln3Δex7/8 mice harbor the most common genetic defect causing juvenile neuronal ceroid lipofuscinosis (JNCL), an autosomal recessive disease involving seizures, visual, motor and cognitive decline, premature death. Here, to more thoroughly investigate manifestations of JNCL mutation, we performed a broad phenotyping study mice. Homozygous mice, congenic on C57BL/6N background, displayed subtle deficits in sensory tasks at 10–14 weeks age. also electroretinographic changes reflecting cone...
Several human diseases are associated with a lack of caveolae. Yet, the functions caveolae and molecular mechanisms critical for shaping them still debated. We show that muscle cells syndapin III KO mice severe reductions reminiscent caveolinopathies. different from other mouse models, levels plasma membrane-associated caveolar coat proteins caveolin3 cavin1 were both not reduced upon KO. This allowed dissecting bona fide those supported by mere caveolin presence also demonstrated neither...
Herein, we develop a novel and effective combination nanoplatform for cancer theranostics. Folic acid (FA) is first modified on the photothermal agent of polydopamine (PDA), which possesses excellent near-infrared (NIR) absorbance thermal conversion features. Temperature-sensitive silver nanoclusters (AgNCs) are then synthesized DNA template that also loads anticancer drug doxorubicin (Dox). After accumulation in cells, PDA generates cytotoxic heat upon excitation NIR light therapy. On other...
Abstract Glioblastoma multiforme (GBM) is the most aggressive brain tumor, with a 5-year survival ratio <5%. Invasive growth major determinant of poor prognosis in GBM. In this study, we demonstrate that high expression PPFIA binding protein 1 (PPFIBP1) correlates remarkable invasion and GBM patients. Using scratch transwell assay, find migration cells are promoted by overexpression PPFIBP1, while inhibited knockdown PPFIBP1. Then, illustrate PPFIBP1 facilitates glioma cell infiltration...
Abstract Background The tumour microenvironment contributes to chemotherapy resistance in gliomas, and glioma-associated mesenchymal stromal/stem cells (gaMSCs) are important stromal cell components that play multiple roles progression. However, whether gaMSCs affect the first-line agent temozolomide (TMZ) remains unclear. Herein, we explored effect mechanism of on TMZ glioma cells. Methods Human (cell line U87MG primary glioblastoma GBM-1) were cultured conditioned media further treated...
Synergetic photothermal/photodynamic/chemotherapy receives significant attention for precise in vivo cancer treatment. Despite plenty of encouraging photosensitizers explored, integrated nanoagents with multiple functions are still highly desired. In this study, novel nanocomposites coupling black phosphorus (BP) nanosheets, gold nanorods (AuNRs), carbon nanodots (CDs), and doxorubicin (Dox) prepared. The exhibit high antitumor activity on account their broad light absorption, excellent...
Under the label of German Mouse Clinic (GMC), a concept has been developed and implemented that allows better understanding human diseases on pathophysiological molecular level. This includes crosstalk between different organs, pleiotropy genes, systemic impact envirotypes drugs. In GMC, experts from various fields mouse genetics physiology, in close collaboration with clinicians, work side by under one roof. The GMC is an open-access platform for scientific community providing phenotypic...
Abstract Human glioma-associated mesenchymal stem cells (gbMSCs) are the stromal cell components that contribute to tumourigenesis of malignant gliomas. Recent studies have shown gbMSCs consist two distinct subpopulations (CD90 + and CD90 − gbMSCs). However, different roles in glioma progression not been expounded. In this study, we found were associated with expression. high significantly drove mainly by increasing proliferation, migration adhesion, where as low contributed chiefly through...
Abstract Background Glioblastoma stem cells (GSCs) and their interplay with tumor-associated macrophages (TAMs) are responsible for malignant growth tumor recurrence of glioblastoma multiforme (GBM), but the underlying mechanisms largely unknown. Methods Cell viability, stemness, migration, invasion were measured in GSCs after knockdown upstream stimulating factor 1 (USF1). Luciferase assay chromatin immunoprecipitation qPCR performed to determine regulation CD90 by USF1....
Cell-laden hydrogel microspheres with uniform size show great potential for tissue repair and drug screening applications. Droplet microfluidic systems have been widely used the generation of cell-laden microspheres. However, existing droplet are mostly based on complex chips not compatible well culture plates. Moreover, produced by microfluidics need demulsification purification from oil, which requires time effort may compromise cell viability. Herein, we present a simple one-step approach...
Introduction Inflammatory bowel disease (IBD) is a chronic involving multiple genes, and the current available targeted drugs for IBD only deliver moderate efficacy. Whether there single gene that systematically regulates not yet known. MiR-146a plays pivotal role in repression of innate immunity, but its function intestinal inflammation sort controversy, genetic regulatory networks regulated by miR-146a has been revealed. Methods RT-qPCR was employed to detect expression patients mouse...
Nucleophosmin 1 (NPM1) is commonly mutated in myelodysplastic syndrome (MDS) and acute myeloid leukemia. Concurrent inflammatory bowel diseases (IBD) MDS are common, indicating a close relationship between IBD MDS. Here we examined the function of NPM1 colitis-associated colorectal cancer (CAC). expression was reduced patients with IBD. Npm1
Significant progress has been made in fabricating porous scaffolds with ultrafine fibers for tissue regeneration. However, the lack of noninvasive tracking methods vivo makes it impossible to track fate such situ. The development near-infrared region II (NIR-II, 1000–1700 nm) dyes provides possibility performing visualization deep-tissue penetration and high spatial resolution vivo. Herein, we developed a polycaprolactone (PCL) ink containing small organic NIR-II dye SY-1030 fluorescently...
Abstract GFP fusion-based fluorescence-detection size-exclusion chromatography (FSEC) has been widely employed for membrane protein expression screening. However, fused itself may occasionally affect the and/or stability of targeted protein, leading to both false-positive and false-negative results in Furthermore, fusion technology is not well suited some proteins, depending on their topology. Here, we developed an FSEC assay utilizing nanobody (Nb) technology, named FSEC-Nb, which proteins...