A5052637470- Radiation Therapy and Dosimetry
- Effects of Radiation Exposure
- Advanced Radiotherapy Techniques
- Molecular Biology Techniques and Applications
- Radiation Effects and Dosimetry
- Carcinogens and Genotoxicity Assessment
- Radiation Dose and Imaging
- Plant Genetic and Mutation Studies
- DNA Repair Mechanisms
- Seed Germination and Physiology
- Radiation Detection and Scintillator Technologies
- Gene expression and cancer classification
- Radiomics and Machine Learning in Medical Imaging
- Cancer, Hypoxia, and Metabolism
- Renal and related cancers
- Light effects on plants
- Coastal and Marine Management
- Computational Drug Discovery Methods
- Mass Spectrometry Techniques and Applications
- Birth, Development, and Health
- RNA Research and Splicing
- Anesthesia and Neurotoxicity Research
UCLouvain
2022-2025
KU Leuven
2021-2025
Institut Universitaire de Recherche Clinique
2022
Ghent University
2014-2021
Belgian Nuclear Research Centre
2014-2021
Ablynx (Belgium)
2017
Ghent University Hospital
2016
The risk of a large-scale event leading to acute radiation exposure necessitates the development high-throughput methods for providing rapid individual dose estimates. Our work addresses three goals, which align with directive European Union's Realizing Network Biodosimetry project (EU-RENB): 1. To examine suitability different gene expression platforms biodosimetry purposes; 2. perform this examination using blood samples collected from prostate cancer patients (in vivo) and healthy donors...
Purpose: RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research emergency response mutual assistance in biodosimetry within EU. Within this extremely active network, number new dosimetry methods have recently been proposed or developed. There requirement to test and/or validate these candidate techniques inter-comparison exercises are well-established method such validation.Materials methods: The authors present details...
Abstract Accurate assessment of the individual exposure dose based on easily accessible samples (e.g. blood) immediately following a radiological accident is crucial. We aimed at developing robust transcription-based signature for biodosimetry from human peripheral blood mononuclear cells irradiated with different doses X-rays (0.1 and 1.0 Gy) rate 0.26 Gy/min. Genome-wide radiation-induced changes in mRNA expression were evaluated both gene exon level. Using exon-specific qRT-PCR, we...
This collaboration of five established European gene expression labs investigated the potential impact culture conditions on transcriptional response peripheral blood to radiation exposure.Blood from one healthy donor was exposed ex vivo a Cobalt 60 source produce calibration curve in addition four unknown doses. After exposure, samples were either diluted with RPMI medium or left untouched. 24-h incubation at 37 °C lysed, while undiluted mixed preservative RNALater and all shipped frozen...
In order to ensure efficient use of medical resources following a radiological incident, there is an urgent need for high-throughput time-efficient biodosimetry tools. the present study, we tested applicability gene expression signature prediction exposure dose as well time elapsed since irradiation.We used whole blood samples from seven healthy volunteers reference (X-ray doses: 0, 25, 50, 100, 500, 1000, and 2000 mGy; points: 8, 12, 24, 36 48 h) other individuals 'blind samples' (20 in...
Understanding the differences in biological response to photon and particle radiation is important for optimal exploitation of therapy cancer patients, as well adequate application protection measures astronauts. To address this need, we compared transcriptional profiles isolated peripheral blood mononuclear cells 8 h after exposure 1 Gy X-rays, carbon ions or iron with those non-irradiated using microarray technology. All genes that were found differentially expressed either type...