A5021084621- Carcinogens and Genotoxicity Assessment
- Effects of Radiation Exposure
- Radiation Effects and Dosimetry
- DNA Repair Mechanisms
- Radiation Dose and Imaging
- Radioactive contamination and transfer
- Radiation Therapy and Dosimetry
- Molecular Biology Techniques and Applications
- Radioactivity and Radon Measurements
- Advanced Radiotherapy Techniques
- Plant Genetic and Mutation Studies
- Nuclear and radioactivity studies
- DNA and Nucleic Acid Chemistry
- Thermoregulation and physiological responses
- Gene expression and cancer classification
- Neutropenia and Cancer Infections
- Statistical Methods in Clinical Trials
- Air Quality and Health Impacts
- Genomic variations and chromosomal abnormalities
- Optical Imaging and Spectroscopy Techniques
- Radiopharmaceutical Chemistry and Applications
- Glutathione Transferases and Polymorphisms
- Chromosomal and Genetic Variations
- Radioactive element chemistry and processing
- Immune responses and vaccinations
Institut de Radioprotection et de Sûreté Nucléaire
2012-2023
McGill University
2021
Institut Gustave Roussy
2010
Service de la Santé Publique
2009
Électricité de France (France)
2005
Centre Hospitalier Universitaire de Poitiers
2004
Recent studies have suggested that visualization of gamma-H2AX nuclear foci can be used to estimate exposure very low doses ionizing radiation. Although this approach is widely for various purposes, its suitability individual human biodosimetry has not yet been assessed. We therefore conducted such an assessment with the help available software observing and automatically scoring foci. The presence was evaluated in peripheral blood lymphocytes exposed ex vivo gamma rays a dose range 0.02 2...
Two quality controlled inter-laboratory exercises were organized within the EU project 'Realizing European Network of Biodosimetry (RENEB)' to further optimize dicentric chromosome assay (DCA) and identify needs for training harmonization activities RENEB network.The general study design included blood shipment, sample processing, analysis aberrations radiation dose assessment. After manual scoring chromosomes in different cell numbers estimations corresponding 95% confidence intervals...
The aim of biological dosimetry is to estimate the dose and associated uncertainty which an accident victim was exposed. This process requires use maximum-likelihood method for fitting a calibration curve, procedure that not implemented in most statistical computer programs. Several laboratories have produced their own programs, but these are frequently user-friendly available outside users. We developed software linear-quadratic dose–response relationship by estimating from number...
After large-scale accidental overexposure to ionizing radiation, a rapid triage of the exposed population can be performed by scoring dicentrics and ring chromosomes among 50 metaphases. This is but not accurate because sensitivity around 0.5 Gy. step, dose estimated 500 lengthy very between 0.1 0.2 To improve methodology, we propose use software for automatic dicentric that was tested on victims an accident in Dakar. Manual metaphases carried out, then manual metaphases, scoring. Comparison...
Purpose: A European network was initiated in 2012 by 23 partners from 16 countries with the aim to significantly increase individualized dose reconstruction case of large-scale radiological emergency scenarios.Results: The built on three complementary pillars: (1) an operational basis seven biological and physical dosimetric assays ready-to-use mode, (2) a for education, training quality assurance, (3) further development regarding new techniques members. Techniques individual estimation...
Purpose: RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research emergency response mutual assistance in biodosimetry within EU. Within this extremely active network, number new dosimetry methods have recently been proposed or developed. There requirement to test and/or validate these candidate techniques inter-comparison exercises are well-established method such validation.Materials methods: The authors present details...
The last decades have seen increased concern about the possible effects of low to moderate doses ionizing radiation (IR) exposure on cognitive function. An interdisciplinary group experts (biologists, epidemiologists, dosimetrists and clinicians) in this field gathered together framework European MELODI workshop non-cancer IR summarise state knowledge topic elaborate research recommendations for future studies area. Overall, there is evidence from both biology epidemiology, though a better...
The aim of this work was to use several new biological indicators evaluate damage the main physiological systems in a victim exposed accidentally ionizing radiation. Blood samples were used for dosimetry and measurement plasma concentrations molecules: Flt3 ligand assess hematopoietic system, citrulline as an indicator digestive tract, oxysterols lipid metabolism vascular markers. cytogenetic evaluation estimated dose be between 4.2 4.8 Gy, depending on methodology used. Monitoring...
In case of an accidental overexposure to ionizing radiation where the dose received by victim is over 5 Gy, conventional biological indicator dose, dicentric assay, does not provide accurate enough measurement. A more appropriate technique measure ring chromosomes in stimulated lymphocytes. Dose-effect relationships were obtained plotting frequencies Premature Chromosome Condensation (PCC)-rings PCC lymphocytes chemical induction with Calyculin vitro, irradiated doses between 25 Gy. Cells...
Well-defined protocols and quality management standards are indispensable for biological dosimetry laboratories. Participation in periodic proficiency testing by interlaboratory comparisons is also required. This harmonization essential if a cooperative network used to respond mass casualty event. Here we present an international intercomparison based on dicentric chromosome analysis dose assessment performed the framework of IAEA Regional Latin American RLA/9/054 Project. The exercise...
Journal Article Biological dosimetry for triage of casualties in a large-scale radiological emergency: capacity the EU member states Get access Andrzej Wojcik, Wojcik * 1Stockholm University, Stockholm, Sweden2Jan Kochanowski Kielce, Poland *Corresponding author: andrzej.wojcik@gmt.su.se Search other works by this author on: Oxford Academic PubMed Google Scholar David Lloyd, Lloyd 3Health Protection Agency (HPA), Chilton, UK Horst Romm, Romm 4Federal Office Radiation (BfS), Salzgitter,...
In accidental exposure to ionizing radiation, it is essential estimate the dose received by victims. Currently dicentric scoring best biological indicator of exposure. The standard dosimetry procedure (500 metaphases scored manually) suitable for a few estimations, but time needed analysis can be problematic in case large-scale accident. Recently, new methodology using automatic detection dicentrics has greatly decreased estimation and preserves accuracy estimation. However, capability...
Biological dosimetry enables individual dose reconstruction in the case of unclear or inconsistent radiation exposure situations, especially when a direct measurement ionizing is not no longer possible. To be prepared for large-scale radiological incidents, networking between well-trained laboratories has been identified as useful approach provision fast and trustworthy assessments needed such circumstances. this end, various biodosimetry worldwide have joined forces set up regional and/or...
Purpose: To record the latest information on control levels of translocations in cultured human lymphocytes.Materials and methods: Control-level data from seven European laboratories that are using fluorescence situ hybridization (FISH) techniques for retrospective biological dosimetry have been combined a meta-analysis. After correction differing probe combinations used, tests consistency performed. The used to test individual variation, systematic variation with age, gender smoking...
Gruel, G., Voisin, P., Vaurijoux, A., Roch-Lefèvre, S., Gré goire, E., Maltère, Petat, C., Gidrol, X., P. and Roy, L. Broad Modulation of Gene Expression in CD4 Lymphocyte Subpopulations Response to Low Doses Ionizing Radiation. Radiat. Res. 170, 335–344 (2008).To compare the responses different lymphocyte subtypes after an exposure whole blood low doses ionizing radiation, we examined variations gene expression subpopulations using microarray technology. Blood samples from five healthy...
Purpose: The fluorescent in situ hybridization (FISH) technique, which easily detects reciprocal translocations, is currently used to estimate doses retrospective biological dosimetry, after suspected accidental overexposure ionizing radiation (IR). This study of 42 cases aimed verify the appropriateness this assay for dose reconstruction, compared dicentric assay, and evaluate other limitations.Material methods: We labeled chromosomes 2, 4, 12 by 3-color FISH painting detect translocations...
Purpose: In the frame of QA program RENEB, an inter-laboratory comparison (ILC) calibration sources used in biological dosimetry was achieved to investigate influence practices and protocols on results dose estimation performance as a first step harmonization standardization irradiation European network.Materials methods: Delivered doses by facilities RENEB partners were determined with EPR/alanine system. Dosimeters irradiated same conditions blood samples. A short survey also performed...
The aim of this work was to evaluate and follow up the evolution radiation damage in two victims a accident. Blood samples were used for cytogenetic evaluation dose heterogeneity. estimates 1 Gy 2.3 most exposed patients. Plasma measurement Flt3 ligand as marker haematopoietic aplasia, citrulline jejunal mucosal epithelium oxysterols liver, central nervous system vascular compartment. use these biological indicators demonstrated presence syndrome suggested subclinical radiation-induced liver...