A5004485615- Carcinogens and Genotoxicity Assessment
- Radiation Effects and Dosimetry
- Radioactive contamination and transfer
- Effects of Radiation Exposure
- Radiation Therapy and Dosimetry
- Radiation Dose and Imaging
- DNA Repair Mechanisms
- Radioactivity and Radon Measurements
- Nuclear Physics and Applications
- Molecular Biology Techniques and Applications
- Bacillus and Francisella bacterial research
- Radiation Detection and Scintillator Technologies
- Advanced Radiotherapy Techniques
- Plant tissue culture and regeneration
- Animal testing and alternatives
- Medical Imaging Techniques and Applications
- Boron Compounds in Chemistry
- Educational Technology and Assessment
- Chemical Safety and Risk Management
- Chromosomal and Genetic Variations
- DNA and Nucleic Acid Chemistry
- Effects and risks of endocrine disrupting chemicals
- Seed Germination and Physiology
- 3D Printing in Biomedical Research
- Nuclear and radioactivity studies
Federal Office for Radiation Protection
2007-2020
Ghent University
2014
Universität Ulm
2014
Iowa State University
1947
Rapid biodosimetry tools are required to assist with triage in the case of a large-scale radiation incident. Here, we aimed determine dose-assessment accuracy well-established dicentric chromosome assay (DCA) and cytokinesis-block micronucleus (CBMN) comparison emerging γ-H2AX foci gene expression assays for mode injury assessment. Coded blood samples exposed 10 X-ray doses (240 kVp, 1 Gy/min) up 6.4 Gy were sent participants dose estimation. Report times documented each laboratory assay....
Two quality controlled inter-laboratory exercises were organized within the EU project 'Realizing European Network of Biodosimetry (RENEB)' to further optimize dicentric chromosome assay (DCA) and identify needs for training harmonization activities RENEB network.The general study design included blood shipment, sample processing, analysis aberrations radiation dose assessment. After manual scoring chromosomes in different cell numbers estimations corresponding 95% confidence intervals...
This interlaboratory comparison validates the dicentric chromosome assay for assessing radiation dose in mass casualty accidents and identifies advantages limitations of an international biodosimetry network. The assay's validity accuracy were determined among five laboratories following International Organization Standardization guidelines. Blood samples irradiated at Armed Forces Radiobiology Research Institute shipped to all laboratories, which constructed individual calibration curves...
The World Health Organization (WHO) held a consultation meeting at WHO Headquarters, Geneva, Switzerland, December 17-18, 2007, to develop the framework for global biodosimetry network. network is envisioned enable dose assessment using multiple methods [cytogenetics, electron paramagnetic resonance (EPR), radionuclide bioassays, etc.]; however, initial discussion focused on cytogenetic bioassay (i.e., metaphase-spread dicentric assay). Few regional networks have been established so far....
The aim of biological dosimetry is to estimate the dose and associated uncertainty which an accident victim was exposed. This process requires use maximum-likelihood method for fitting a calibration curve, procedure that not implemented in most statistical computer programs. Several laboratories have produced their own programs, but these are frequently user-friendly available outside users. We developed software linear-quadratic dose–response relationship by estimating from number...
Purpose: In case of a large-scale radiation accident when hundreds people may be exposed, it is important to distinguish the severely exposed individuals (≥1 gray), who require early medical treatment, from those less exposed. The aim our study was develop quick population triage method based on automated micronucleus (MN) scoring.Materials and methods: Using MN software module developed by MetaSystems specifically for Metafer4 platform, about 60 blood samples can scored in one day. Standard...
Biological dosimetry is an essential tool for estimating radiation dose. The dicentric chromosome assay (DCA) currently the of choice. Because labor-intensive and time-consuming, strategies are needed to increase throughput use in mass casualty incidents. One such strategy truncate metaphase spread analysis triage dose estimates by scoring 50 or fewer metaphases, compared a routine 500 1000 using large group scorers biodosimetry network. Previously, National Institutes Allergies Infectious...
In Europe, a network for biological dosimetry has been created to strengthen the emergency preparedness and response capabilities in case of large-scale nuclear accident or radiological emergency. Through RENEB (Realising European Network Biodosimetry) project, 23 experienced laboratories from 16 countries will establish sustainable rapid, comprehensive standardised biodosimetry provision that would be urgently required an situation on ground. The foundation is formed by five main pillars:...
Purpose: A European network was initiated in 2012 by 23 partners from 16 countries with the aim to significantly increase individualized dose reconstruction case of large-scale radiological emergency scenarios.Results: The built on three complementary pillars: (1) an operational basis seven biological and physical dosimetric assays ready-to-use mode, (2) a for education, training quality assurance, (3) further development regarding new techniques members. Techniques individual estimation...
The study design and obtained results represent an intercomparison of various laboratories performing dose assessment using the dicentric chromosome analysis (DCA) as a diagnostic triage tool for individual radiation assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples establishing calibration data (0.25–5 Gy) well blind (0.1–6.4 were sent to participants. DCA was performed according established protocols. time taken report estimates documented each laboratory. Additional...
Well-defined protocols and quality management standards are indispensable for biological dosimetry laboratories. Participation in periodic proficiency testing by interlaboratory comparisons is also required. This harmonization essential if a cooperative network used to respond mass casualty event. Here we present an international intercomparison based on dicentric chromosome analysis dose assessment performed the framework of IAEA Regional Latin American RLA/9/054 Project. The exercise...
Purpose: The European Union's Seventh Framework Programme-funded project 'Multi-disciplinary biodosimetric tools to manage high scale radiological casualties' (MULTIBIODOSE) has developed a multiparametric approach radiation biodosimetry, with particular emphasis on triage of large numbers potentially exposed individuals following accidental exposures. In November 2012, an emergency exercise took place which tested the capabilities MULTIBIODOSE partners. described here had dual purpose:...
The focus of the study is an intercomparison laboratories' dose-assessment performances using cytokinesis-block micronucleus (CBMN) assay as a diagnostic triage tool for individual radiation dose assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples establishing calibration data (0.25-5 Gy) well blind (0.1-6.4 were sent to participants. CBMN was performed according protocols individually established and varying among participating laboratories. time taken report estimates...
Creating a sustainable network in biological and retrospective dosimetry that involves large number of experienced laboratories throughout the European Union (EU) will significantly improve accident emergency response capabilities case large-scale radiological emergency. A well-organised cooperative action involving EU offer best chance for fast trustworthy dose assessments are urgently needed an situation. To this end, EC supports establishment (RENEB). The RENEB project started January...
Journal Article Biological dosimetry for triage of casualties in a large-scale radiological emergency: capacity the EU member states Get access Andrzej Wojcik, Wojcik * 1Stockholm University, Stockholm, Sweden2Jan Kochanowski Kielce, Poland *Corresponding author: andrzej.wojcik@gmt.su.se Search other works by this author on: Oxford Academic PubMed Google Scholar David Lloyd, Lloyd 3Health Protection Agency (HPA), Chilton, UK Horst Romm, Romm 4Federal Office Radiation (BfS), Salzgitter,...
Journal Article Is a semi-automated approach indicated in the application of automated micronucleus assay for triage purposes? Get access H. Thierens, Thierens * 1Department Basic Medical Sciences, Ghent University, Building 5B3, De Pintelaan 185, Gent B-9000, Belgium *Corresponding author: hubert.thierens@ugent.be Search other works by this author on: Oxford Academic PubMed Google Scholar A. Vral, Vral C. Vandevoorde, Vandevoorde V. Vandersickel, Vandersickel de Gelder, Gelder Romm, Romm...