A5021856310- Carcinogens and Genotoxicity Assessment
- Effects of Radiation Exposure
- Radiation Effects and Dosimetry
- DNA Repair Mechanisms
- Radioactive contamination and transfer
- Radiation Therapy and Dosimetry
- Cancer Genomics and Diagnostics
- Radiation Dose and Imaging
- Plant Genetic and Mutation Studies
- Advanced Radiotherapy Techniques
- Cell Image Analysis Techniques
- Medical and Agricultural Research Studies
- Radiomics and Machine Learning in Medical Imaging
- Childhood Cancer Survivors' Quality of Life
- Cancer Immunotherapy and Biomarkers
- AI in cancer detection
- Radioactivity and Radon Measurements
- Bacillus and Francisella bacterial research
- Chemical Reactions and Isotopes
- Mathematical Biology Tumor Growth
State Organization «Grigoriev Institute for Medical Radiology and Oncology of the National Academy of Medical Sciences of Ukraine»
2010-2024
Kharkiv National Medical University
2013-2024
Kharkiv Medical Academy of Postgraduate Education
2022
National Academy of Medical Sciences of Ukraine
2021
Academy of Medical Sciences
2010-2013
Two quality controlled inter-laboratory exercises were organized within the EU project 'Realizing European Network of Biodosimetry (RENEB)' to further optimize dicentric chromosome assay (DCA) and identify needs for training harmonization activities RENEB network.The general study design included blood shipment, sample processing, analysis aberrations radiation dose assessment. After manual scoring chromosomes in different cell numbers estimations corresponding 95% confidence intervals...
The World Health Organization (WHO) held a consultation meeting at WHO Headquarters, Geneva, Switzerland, December 17-18, 2007, to develop the framework for global biodosimetry network. network is envisioned enable dose assessment using multiple methods [cytogenetics, electron paramagnetic resonance (EPR), radionuclide bioassays, etc.]; however, initial discussion focused on cytogenetic bioassay (i.e., metaphase-spread dicentric assay). Few regional networks have been established so far....
Tools for radiation exposure reconstruction are required to support the medical management of victims in radiological or nuclear incidents. Different biological and physical dosimetry assays can be used various scenarios estimate dose ionizing a person has absorbed. Regular validation techniques through inter-laboratory comparisons (ILC) is essential guarantee high quality results. In current RENEB comparison, performance established cytogenetic [dicentric chromosome assay (DCA),...
Journal Article The capacity, capabilities and needs of the WHO biodosenet member laboratories Get access N. A. Maznyk, Maznyk 1Institute for Medical Radiology Academy Science Ukraine, Pushkinskaya St. 82, Kharkiv 61024, Ukraine Search other works by this author on: Oxford Academic PubMed Google Scholar R. C. Wilkins, Wilkins * 2Health Canada, Ottawa, ON, CanadaK1A 0K9 *Corresponding author: ruth.wilkins@hc-sc.gc.ca Z. Carr, Carr 3World Health Organization, Geneva, Switzerland D. Lloyd...
The dose response for dicentrics plus centric rings and total unstable chromosome-type aberrations was studied in the first mitoses of cultured human peripheral blood lymphocytes irradiated vitro to doses ∼2, 4, 6, 8, 10, 16 20 Gy acute 60Со gamma-rays. A dose-dependent increase aberration yield accompanied by a tendency underdispersion among cells distributions compared with Poisson statistics at ≥6 Gy. formal fitting data linear-quadratic model resulted an equation linear quadratic...
After large-scale radiation accidents where many individuals are suspected to be exposed ionizing radiation, biological and physical retrospective dosimetry assays important tools aid clinical decision making by categorizing into unexposed/minimally, moderately or highly groups. Quality-controlled inter-laboratory comparisons of simulated accident scenarios regularly performed in the frame European legal association RENEB (Running Network Biological Physical Dosimetry) optimize international...
The Poisson distribution is the most widely recognised and commonly used for cytogenetic radiation biodosimetry. However, it that, due to complexity of exposure cases, other distributions may be more properly applied. Here, Poisson, gamma, negative binomial, beta, Neyman type-A Hermite are compared in terms their applicability 'real-life' situations. identification appropriate statistical model each particular situation correctly characterises data. results show that acute, homogeneous...
Purpose: To assess possible delayed chromosomal instability (DCI) expressed as elevated chromatid breakage in cells containing previously formed chromosome type aberrations cultured blood lymphocytes of cancer patients after radiotherapy (RT).Materials and methods: Twenty treated for uterine with external Co60 RT, without chemotherapy, were selected. Blood was taken before, 1–2 days RT one year later. Lymphocytes 50 100 h. Metaphases stained fluorescence-plus-Giemsa analysed 1st (M1) 3rd...
In 2008 the World Health Organization established an international network of biodosimetry laboratories, BioDoseNet. The goal this is to support cooperation and capacity building in area biodosimetry, including harmonisation protocols techniques enable them provide mutual assistance during a mass casualty event. Since its inception, evolution, success needs have been monitored by multiple surveys conducted approximately every five years. third such survey was 2021, now allowing trends...
ЦИТОГЕНЕТИЧНІ ПОШКОДЖЕННЯ У ХВОРИХ НА
After Chernobyl accident, the cohort and individual dose estimations for various groups of exposed population were great importance. The biological dosimetry based on chromosome aberrations study was technique choice evaluating radiation exposure in persons who took part clean-up activities post-critical following periods (liquidators). For subsequent liquidators group additional task biodosimetry verification doses registered military or other official documents. Cytogenetic investigations...
The problem of delayed genomic instability occurring in human cells vivo has recently come to prominence as an important mechanism radiation carcinogenesis. Considering cancer patients treated with partial body irradiation a relevant group at risk developing second cancer, research been carried out quantify chromosomal (ChI) their lymphocytes. Ten uterine were selected the IMR clinic according local ethics procedures minimum variations age (55-65 y), tumor grade (T2a-cNxM0) and treatment...
Aims: The assessment of radiation-induced chromosome aberrations in lymphocytes oncogynecological and head neck cancer patients during radiation therapy on linear accelerator depending the tumor localization.Methods: 16 12 with tumors undergoing treatment were examined. Lymphocytes cultivated by conventional technique before treatment, middle at end radiotherapy course. Radiation doses exceeded 40–44 Gy.Results research: yield range cytogenetic damage changes megavolt demonstrated. monotonic...
Aims: The detection of chromosome type aberrations outcome in oncogynecological patients during radiation therapy depending on the irradiation regime.Methods: group 36 was examined before treatment, middle and at end external gamma-radiotherapy 60Со ROCUS-AM megavolt linear accelerator Clinac 600C. Lymphocytes were cultivated by conventional technique 50 – 54 hours. Chromosome detected with classical cytogenetic analysis.Results research: changes lymphocytes 600C demonstrated. excess damage...
Aims: Estimation of the cytogenetic lesions yield and their distribution among cells in donor lymphocytes cancer patients with different tumor localizations depending on source radiation locality exposure a therapeutically significant dose ex vivo experiment.Methods: Cytogenetic analysis was performed 30 oncogynecological patients, lung head neck before start treatment. Whole peripheral blood irradiated at 2 Gy further simulation partial body irradiation using gamma-irradiation 60Co ROKUS-AM...
Background. Radiotherapy can be used numerous times for treating oncological patients as one of the most effective ways fighting against cancer. However, there is limited data on effect prior radiation exposure further development cytogenetic alterations under influence radiological factors. Primary treatment, well second one, an example a complex scenario fractionated irradiation, examination which fundamental and practical importance understanding processes formation elimination markers...