A5059306730- Nanoparticles: synthesis and applications
- Genomics, phytochemicals, and oxidative stress
- Radiation Effects and Dosimetry
- Molecular Biology Techniques and Applications
- Iron Metabolism and Disorders
- Erythropoietin and Anemia Treatment
- Carcinogens and Genotoxicity Assessment
- Nanoparticle-Based Drug Delivery
- Graphene and Nanomaterials Applications
- Trace Elements in Health
- Radiation Therapy and Dosimetry
- Nitric Oxide and Endothelin Effects
- DNA Repair Mechanisms
- NF-κB Signaling Pathways
- Epigenetics and DNA Methylation
- Obstructive Sleep Apnea Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer therapeutics and mechanisms
- MicroRNA in disease regulation
- Energy and Environment Impacts
- Heart Failure Treatment and Management
- Cancer, Lipids, and Metabolism
- Alzheimer's disease research and treatments
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
Instytut Chemii i Techniki Jądrowej
2015-2024
Central Laboratory for Radiological Protection
2011
University of Warsaw
2007
Iron-sulfur clusters-containing proteins participate in many cellular processes, including crucial biological events like DNA synthesis and processing of dioxygen. In most iron-sulfur proteins, the clusters function as electron-transfer groups mediating one-electron redox processes such they are integral components respiratory photosynthetic electron transfer chains numerous enzymes involved carbon, oxygen, hydrogen, sulfur nitrogen metabolism. Recently, novel regulatory enzymatic functions...
Purpose: RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research emergency response mutual assistance in biodosimetry within EU. Within this extremely active network, number new dosimetry methods have recently been proposed or developed. There requirement to test and/or validate these candidate techniques inter-comparison exercises are well-established method such validation.Materials methods: The authors present details...
This collaboration of five established European gene expression labs investigated the potential impact culture conditions on transcriptional response peripheral blood to radiation exposure.Blood from one healthy donor was exposed ex vivo a Cobalt 60 source produce calibration curve in addition four unknown doses. After exposure, samples were either diluted with RPMI medium or left untouched. 24-h incubation at 37 °C lysed, while undiluted mixed preservative RNALater and all shipped frozen...
The increasing use of nanoparticles (NPs) in various applications entails the need for reliable assessment their potential toxicity humans. Originally, studies concerning NPs focused on cytotoxic and genotoxic effects, but more recently, attention has been paid to epigenetic changes induced by nanoparticles. In present research, we analysed DNA methylation status genes related inflammation apoptosis as well expression miRNAs these processes response silver (AgNPs), gold (AuNPs),...
Abstract Large-scale radiation emergency scenarios involving protracted low dose rate exposure (e.g. a hidden radioactive source in train) necessitate the development of high throughput methods for providing rapid individual estimates. During RENEB (Running European Network Biodosimetry) 2019 exercise, four EDTA-blood samples were exposed to an Iridium-192 (1.36 TBq, Tech-Ops 880 Sentinal) at varying distances and geometries. This resulted doses ranging between 0.2 2.4 Gy using rates 1.5–40...
Early and high-throughput individual dose estimates are essential following large-scale radiation exposure events. In the context of Running European Network for Biodosimetry Physical Dosimetry (RENEB) 2021 exercise, gene expression assays were conducted their corresponding performance dose-assessment is presented in this publication. Three blinded, coded whole blood samples from healthy donors exposed to 0, 1.2 3.5 Gy X-ray doses (240 kVp, 1 Gy/min) using source Yxlon. These exposures...
Tools for radiation exposure reconstruction are required to support the medical management of victims in radiological or nuclear incidents. Different biological and physical dosimetry assays can be used various scenarios estimate dose ionizing a person has absorbed. Regular validation techniques through inter-laboratory comparisons (ILC) is essential guarantee high quality results. In current RENEB comparison, performance established cytogenetic [dicentric chromosome assay (DCA),...
Biodiesels represent more carbon-neutral fuels and are introduced at an increasing extent to reduce emission of greenhouse gases. However, the potential impact different types blend concentrations biodiesel on toxicity diesel engine emissions still relatively scarce some contradictory. The objective present work was compare exhaust particles (DEP) from combustion two 1st-generation fuels: 7% fatty acid methyl esters (FAME; B7) 20% FAME (B20) a 2nd-generation FAME/HVO (synthetic hydrocarbon...
The most frequently used and the best established method of biological dosimetry at present is dicentric chromosome assay, which poorly suitable for a mass casualties scenario. This gives rise to need development new, high-throughput assays rapid identification subjects exposed ionizing radiation. In study, we tested usefulness gene expression analysis in blood cells dosimetry. Human peripheral from three healthy donors was X-irradiated with doses 0 (control), 0.6, 2 Gy. mRNA level 16 genes...
Epidemiological data indicate that exposure to diesel exhaust particles (DEPs) from traffic emissions is associated with higher risk of morbidity and mortality related cardiovascular pulmonary diseases, accelerated progression atherosclerotic plaques, possible lung cancer. While the impact DEPs combustion fossil fuel on human health has been extensively studied, current knowledge biofuels provides limited inconsistent information about its mutagenicity genotoxicity, as well adverse risks....
Silver nanoparticles (AgNPs) are one of the most widely used nanomaterials due to their antibacterial properties. Owing recent boost in usage AgNPs-containing products, human exposure AgNPs is increasing, highlighting need for careful evaluation toxicity humans. We two cellular models, hepatic HepG2 and epithelial A549 cell lines, study mechanism AgNPs-induced at level. These lines differ significantly response treatment. In case cells, a minor decrease viability increase extent DNA breakage...
Pirin, a product of the PIR gene, is an iron-binding protein acting as transcriptional coregulator implicated in regulation NF-κB-related transcription via interaction with RelA (p65), well BCL3 and NF-κB1 (p50) proteins. Alterations pirin expression were observed various tumors under oxidative stress conditions. The aim present work was to analyze human gene. Using constructs containing different sized promoter luciferase reporter genes we found that HeLa cells mostly dependent on highly...
Morphological studies of 16 strains belonging to the genus Monomorphina revealed a single, parietal, orbicular chloroplast in their cells. The has tendency be perforated and disintegrates aging populations thus may appear many chloroplasts under light microscope. A single cells Cryptoglena skujae is also parietally located highly perforated. It never forms globular closed structure, but open from side furrow, resembling letter C . We have verified pyrum group ( M. –like) on basis...
Alzheimer’s disease (AD) is a chronic neurodegenerative leading to progressive dementia in elderly people. The characterized, among others, by formation of amyloid- β (A β) polypeptide plaques the brain. Although etiology not fully understood, recent research suggest that nanomaterials may affect AD development. Here, we described consequences exposure mouse BV-2 microglia silver nanoparticles (AgNPs, 50 µg/mL), cerium oxide (CeO 2 NPs, 100 and cadmium telluride quantum dots (CdTeQDs, 3 or...
Metastatic castration-resistant prostate cancer (mCRPC) is a progressive and incurable disease with poor prognosis for patients. Despite introduction of novel therapies, the mortality rate remains high. An attractive alternative extension life mCRPC patients PSMA-based targeted radioimmunotherapy. In this paper, we extended our in vitro study 223Ra-labeled PSMA-targeted NaA nanozeolites [223RaA-silane-PEG-D2B] by undertaking comprehensive preclinical vivo research. The toxicity new compound...
Iron binding protein pirin was isolated as an interactor of the NFIX transcription factor but it can also form complexes with Bcl3 and NF-κB1(p50). Alterations expression were observed in various tumors after exposure to pro-carcinogenic oxidative stressors. The aim present work study level vivo model stress, namely, Sod1-deficient mice. We have found that Sod1−/− mice a significantly elevated Pir mRNA spleen kidney not liver, heart, or/and brain. shown similarly its human ortholog, mouse...