A5048248689- DNA Repair Mechanisms
- Radiation Therapy and Dosimetry
- Plant Genetic and Mutation Studies
- PARP inhibition in cancer therapy
- Carcinogens and Genotoxicity Assessment
- Genomics and Chromatin Dynamics
- Ion-surface interactions and analysis
- Effects of Radiation Exposure
- Advanced Radiotherapy Techniques
- DNA and Nucleic Acid Chemistry
- Radiation Dose and Imaging
- Fungal and yeast genetics research
- Laser-Plasma Interactions and Diagnostics
- CRISPR and Genetic Engineering
- Heat shock proteins research
- Radiation Effects and Dosimetry
- S100 Proteins and Annexins
- Radioactive contamination and transfer
- Epigenetics and DNA Methylation
- Integrated Circuits and Semiconductor Failure Analysis
- Molecular Biology Techniques and Applications
- Breast Cancer Treatment Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Telomeres, Telomerase, and Senescence
- Microtubule and mitosis dynamics
Ludwig-Maximilians-Universität München
2015-2024
LMU Klinikum
2012-2023
Helmholtz Zentrum München
2014-2022
Stockholm University
2022
Klinikum rechts der Isar
2019
Klinik und Poliklinik für Strahlentherapie und Radioonkologie
2012
Center for Integrated Protein Science Munich
2011
München Klinik
2011
Institut für Urheber- und Medienrecht
2006-2010
University of Wisconsin–Madison
1999-2009
Radiation exposures at ultrahigh dose rates (UHDRs) several orders of magnitude greater than in current clinical radiotherapy (RT) have been shown to manifest differential radiobiological responses compared conventional (CONV) rates. This has led studies investigating the application UHDR for therapeutic advantage (FLASH-RT) that gained significant interest since initial discovery 2014 demonstrated reduced lung toxicity with equivalent levels tumor control dose-rate RT. Many subsequent...
In mammalian cells, all subunits of the DNA-dependent protein kinase (DNA-PK) have been implicated in repair DNA double-strand breaks and V(D)J recombination. yeast Saccharomyces cerevisiae, we examined phenotype conferred by a deletion HDF1, putative homologue 70-kD subunit DNA-end binding Ku complex DNA-PK. The gene does not play role radiation-induced cell cycle checkpoint arrest G1 G2 or hydroxyurea-induced S. cells competent for homologous recombination, could detect any sensitivity to...
Ion beams are relevant for radiobiological studies and tumor therapy. In contrast to conventional accelerators, laser-driven ion acceleration offers a potentially more compact cost-effective means of delivering ions radiotherapy. Here, we show that by combining advanced using nanometer thin targets beam transport, truly nanosecond quasi-monoenergetic proton bunches can be generated with table-top laser system, single shot doses up 7 Gy living cells. Although in their infancy, laser-ion...
Laser acceleration of protons and heavy ions may in the future be used radiation therapy. Laser-driven particle beams are pulsed ultra high dose rates >10⁹ Gy s⁻¹ achieved. Here we compare radiobiological effects continuous proton beams.The ion microbeam SNAKE at Munich tandem accelerator was to directly a 20 MeV beam, which delivered 3 HeLa cell monolayer within < 1 ns or 100 ms, respectively. Investigated endpoints were G2 phase cycle arrest, apoptosis, colony formation.At 10 h after...
Abstract The spatial distribution of DSB repair factors γH2AX, 53BP1 and Rad51 in ionizing radiation induced foci (IRIF) HeLa cells using super resolution STED nanoscopy after low high linear energy transfer (LET) irradiation was investigated. γH2AX form IRIF with same mean size (540 ± 40) nm LET while the is significantly smaller. both show nanostructures partial anti-correlation. These structures are related to domains formed within chromatin territories marked by mainly situated...
Purpose: In order to obtain more insight into heavy ion tumour therapy, some features of the underlying molecular mechanisms controlling cellular response high linear energy transfer (LET) radiation are currently analysed.Materials and methods: We analysed decay integrated fluorescence intensity γ-H2AX (phosphorylated histone H2AX) which is thought reflect repair kinetics radiation-induced DNA double-strand breaks (DSB) using Laser-Scanning-Cytometry. Asynchronous human HeLa cells were...
High-linear energy transfer (LET) ion irradiation of cell nuclei induces complex and severe DNA lesions, foci repair proteins are formed densely along the trajectory. To efficiently discriminate distributed/overlapping trajectory, a focus recognition algorithm called FociPicker3D based on local fraction thresholding technique was developed. We analyzed high-resolution 3D immunofluorescence microscopic images obtained kinetics spatial development γ-H2AX, 53BP1 phospho-NBS1 in BJ1-hTERT cells...
Active chromatin remodelling is integral to the DNA damage response in eukaryotes, as sensors, signalling molecules and repair enzymes gain access lesions. A variety of nucleosome complexes known promote different stages repair. The sliding factors CHRAC/ACF Drosophila are involved organization during development. Involvement corresponding hACF1-containing mammalian replication, transcription very recently also non-homologous end-joining breaks have been suggested. We now found that more...
// Stephanie Wurster 1, * , Fabian Hennes Ann C. Parplys 1 Jasna I. Seelbach Wael Y. Mansour 2 Alexandra Zielinski Cordula Petersen 3 Till S. Clauditz 4 Adrian Münscher 5 Anna A. Friedl 6, 7 Kerstin Borgmann Laboratory of Radiobiology and Experimental Radiooncology, Center Oncology, University Medical Hamburg-Eppendorf, Hamburg, Germany Tumor Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt Department Radiotherapy Pathology, Otorhinolaryngology Head Neck Surgery,...
The recruitment kinetics of double-strand break (DSB) signaling and repair proteins Mdc1, 53BP1 Rad52 into radiation-induced foci was studied by live-cell fluorescence microscopy after ion microirradiation. To investigate the influence damage density complexity on kinetics, which cannot be done UV laser irradiation used in former studies, we utilized 43 MeV carbon ions with high linear energy transfer per (LET = 370 keV/µm) to create a large fraction clustered DSBs, thus forming complex DNA...
Poly(ADP-ribose) polymerase 1 (PARP1) is a key player in DNA repair, genomic stability and cell survival it emerges as highly relevant target for cancer therapies. To deepen our understanding of PARP biology mechanisms action PARP1-targeting anti-cancer compounds, we generated novel PARP1-affinity reagent, active both vitro live cells. This PARP1-biosensor based on PARP1-specific single-domain antibody fragment (~ 15 kDa), termed nanobody, which recognizes the N-terminus human PARP1 with...
To obtain greater insight into the future potential of tumor radiotherapy using proton beams generated from high-intensity lasers, it is important to characterize ionization quality new by measuring relative biological effectiveness (RBE) under conditions where full dose at one irradiation site will be deposited a few pulses less than 1 ns in duration. HeLa cells attached Mylar foil were irradiated with 70 kV X rays reference dose–response curve or 3 Gy 20 MeV protons Munich tandem...
Laser accelerated radiotherapy is a potential cancer treatment with proton and carbon-ion beams that currently under development. Ultra-fast high-energy laser pulses will accelerate ion deliver their dose to patient in "pulsed mode" expected differ from conventional irradiation by increasing the delivery rate tissue voxel approximately 8 orders of magnitude. In two independently performed experiments at microprobe SNAKE 14 MV Munich tandem accelerator, A(L) cells were exposed either protons...
Abstract The mobility of damaged chromatin regions in the nucleus may affect probability mis-repair. In this work, live-cell observation and distance tracking GFP-tagged DNA damage response protein MDC1 was used to study random-walk behaviour domains containing radiation-induced double-strand breaks (DSB). Our measurements indicate a subdiffusion-type random walk process with similar time dependence for isolated clustered DSBs that were induced by 20 MeV proton or 43 carbon ion...
The development from single shot basic laser plasma interaction research toward experiments in which repetition rated laser-driven ion sources can be applied requires technological improvements. For example, the case of radio-biological experiments, irradiation duration and reproducible controlled conditions are important for performing studies with a large number samples. We present advancements recent years at ATLAS 300 Garching near Munich since our last radiation biology experiment....