A5079884607- Cancer Immunotherapy and Biomarkers
- Prostate Cancer Treatment and Research
- Neuroendocrine Tumor Research Advances
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Cancer Genomics and Diagnostics
- HER2/EGFR in Cancer Research
- Head and Neck Cancer Studies
- Pancreatic and Hepatic Oncology Research
- Neuroblastoma Research and Treatments
- Cancer-related Molecular Pathways
- Cancer and Skin Lesions
- Cancer-related gene regulation
- Glycosylation and Glycoproteins Research
- Molecular Biology Techniques and Applications
- Lung Cancer Research Studies
- Renal and related cancers
- Salivary Gland Tumors Diagnosis and Treatment
- Gastric Cancer Management and Outcomes
- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Single-cell and spatial transcriptomics
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
Universität Hamburg
2016-2025
University Medical Center Hamburg-Eppendorf
2016-2025
Taipei Institute of Pathology
2016-2021
Klinikum Itzehoe
2021
University Cancer Center Hamburg
2014-2021
Martini-Klinik
2016
University of Göttingen
2006
Mesothelin (MSLN) represents an attractive molecule for targeted cancer therapies. To identify tumors that might benefit from such therapies, tissue microarrays including 15,050 122 different tumor types and 76 healthy organs were analyzed MSLN expression by immunohistochemistry. Sixty-six (54%) showed at least occasional weak staining, 50 (41%) with one strongly positive sample. Highest prevalence of positivity had ovarian carcinomas (serous 97%, clear cell 83%, endometrioid 77%, mucinous...
Trophoblast cell surface antigen 2 (TROP2) is the target of sacituzumab govitecan, an antibody-drug conjugate approved for treatment triple negative breast cancer and urothelial carcinoma.A tissue microarray containing 18,563 samples from 150 different tumor types subtypes as well 608 76 normal was analyzed by TROP2 immunohistochemistry.TROP2 positivity found in 109 categories, including squamous carcinomas various origins, urothelial, breast, prostate, pancreatic, ovarian cancers (>95%...
Introduction: GATA3 is a transcription factor involved in epithelial cell differentiation. immunostaining used as diagnostic marker for breast and urothelial cancer but can also occur other neoplasms. Methods: To evaluate normal tumor tissues, tissue microarray containing 16,557 samples from 131 different types subtypes 608 of 76 was analyzed by immunohistochemistry. Results: positivity found 69 including 23 (18%) with at least one strongly positive tumor. Highest rates occurred noninvasive...
Abstract Objective The disease specific five-year survival rate especially for patients with advanced oral cancer has not improved significantly over the period of time. most effective way combating this dilemma is an early detection, diagnosis and eradication early-stage lesions their precursors. use VELscope ® using autofluorescence as a diagnostic tool might be useful in detection malignant lesions. Materials methods 120 suspicious premalignant were examined two examination methods. They...
IMP3 is an RNA binding protein required for ribosomal processing, which has been suggested to be a prognostic marker in large variety of human types cancer. However, available data on the prevalence expression are largely discrepant. To systematically investigate epidemiology and clinical relevance cancers we employed two-step tissue microarrays (TMAs) approach. First, normal TMA multi-tumor were analyzed immunohistochemically detectable 76 different 3889 cancer samples from 95 tumor...
Biglycan (BGN), a proteoglycan of the extracellular matrix, is included in mRNA signatures for prostate cancer aggressiveness. To understand impact BGN on prognosis and its relationship to molecularly defined subsets, we analyzed expression by immunohistochemistry tissue microarray containing 12,427 cancers. Seventy-eight percent 11,050 interpretable cancers showed expression, which was considered as low intensity 47.7% high 31.1% protein rose with increasing pathological tumor stage,...
Syndecan-1 (CD138) is a transmembrane proteoglycan known to be expressed in various normal and malignant tissues. It of interest because possible prognostic role differential expression tumors its as target for indatuximab, monoclonal antibody coupled with cytotoxic agent. To comprehensively analyze CD138 neoplastic tissues, we used tissue microarrays (TMAs) analyzing immunohistochemically detectable 2,518 samples from 85 different tumor entities 76 types. The data showed that abundant...
Abstract Background Tumor protein 63 (p63) is a transcription factor of the p53 gene family involved in differentiation several tissues including squamous epithelium. p63 immunohistochemistry broadly used for tumor classification but published data on its expression cancer conflicting. Methods To comprehensively catalogue expression, tissue microarrays (TMAs) containing 12,620 samples from 115 entities and 76 normal types were analyzed. Results was seen various epithelium urothelium. At...
Abstract Background Cytokeratin 18 (CK18) is an intermediate filament protein of the cytokeratin acidic type I group and primarily expressed in single-layered or “simple” epithelial tissues carcinomas different origin. Methods To systematically determine CK18 expression normal cancerous tissues, 11,952 tumor samples from 115 types subtypes (including carcinomas, mesenchymal biphasic tumors) as well 608 76 tissue were analyzed by immunohistochemistry a microarray format. Results was cells...
Abstract Background The E-Cadherin gene ( CDH1, Cadherin 1 ), located at 16q22.1 encodes for a calcium-dependent membranous glycoprotein with an important role in cellular adhesion and polarity maintenance. Methods To systematically determine protein expression normal cancerous tissues, 14,637 tumor samples from 112 different types subtypes as well 608 of 76 tissue were analyzed by immunohistochemistry microarray format. Results was strongly expressed epithelial cells most organs. From 77...
Expansion of CD8+ cytotoxic Tlymphocytes is a prerequisite for anti-cancer immune activity and has gained interest in the era checkpoint therapy.To understand T cell dynamics tumor microenvironment, we used multiplex fluorescence immunohistochemistry to quantitate proliferation (Ki67 co-expression) tissue microarrays from 1107 colorectal, 642 renal cell, 1066 breast, 375 ovarian, 451 pancreatic 347 gastric cancer samples.The density percentage proliferating (Ki67+) cells were both highly...
Combined analysis of cytokeratin 7 (CK7) and 20 (CK20) is often used for assessing the origin metastatic cancer. To evaluate diagnostic utility CK7 CK20, tissue microarrays containing 15,424 samples from 120 different tumor types subtypes 608 76 normal were analyzed by immunohistochemistry. positivity was seen in 52% (8.7% weak, 5.9% moderate, 37% strong) CK20 23% (5.1% 3.4% 15% interpretable tumors. Of 8390 positive tumors, 1181 (14%) showed 5380 (64%) alone, 1829 (22%) alone. predominated...
The Ki-67 labeling index (Ki-67 LI) is a strong prognostic marker in prostate cancer, although its analysis requires cumbersome manual quantification of immunostaining 200-500 tumor cells. To enable automated LI assessment routine clinical practice, framework for quantification, which comprises three different artificial intelligence steps and an algorithm cell-distance multiplex fluorescence immunohistochemistry (mfIHC) staining, was developed validated cohort 12,475 cancers. impact the...
Trichorhinophalangeal syndrome 1 (TRPS1) is a nuclear protein highly expressed in breast epithelial cells. TRPS1 immunohistochemistry (IHC) has been suggested as cancer marker. To determine the diagnostic and prognostic utility of IHC, tissue microarrays containing 19,201 samples from 152 different tumor types subtypes were analyzed. GATA3 IHC was performed previous study. staining seen 86 categories with 36 at least one strongly positive case. predominated various carcinomas (51%-100%),...
Cadherin-17 (CDH17) is a membranous cell adhesion protein predominantly expressed in intestinal epithelial cells. CDH17 therefore considered possible diagnostic and therapeutic target. This study was to comprehensively determine the expression of cancer further assess utility immunohistochemistry (IHC). A tissue microarray containing 14,948 interpretable samples from 150 different tumor types subtypes as well 76 normal analyzed by IHC. In tissues, staining seen epithelium intestine...
Objective There is a strong clinical association between IBD and primary sclerosing cholangitis (PSC), chronic disease of the liver characterised by biliary inflammation that leads to strictures fibrosis. Approximately 60%�80% people with PSC will also develop (PSC-IBD). One hypothesis explaining this would be drives IBD. Therefore, our aim was test decipher underlying mechanism. Design Colitis severity analysed in experimental mouse models colitis cholangitis, PSC-IBD. Foxp3 + Treg-cell...
In lung cancer, interleukin-22 (IL-22) expression within primary tissue has been demonstrated, but the frequency and functional consequence of IL-22 signaling have not addressed. This study aims at analyzing cellular effects on carcinoma cell lines prognostic impact in cancer patients.Biological were investigated seven by Western blot, flow cytometry, real-time polymerase chain reaction, proliferation assays. Tumor specimens two cohorts with a total 2300 patients tested for...
DNA mismatch repair (MMR) is integral to the maintenance of genetic stability.We aimed evaluate clinical impact MMR gene expression in prostate cancer.The genes MSH6, MLH1 and PMS2 were analyzed by immunohistochemistry on a tissue microarray containing 11 152 cancer specimens.Results compared with ETS-related status deletions PTEN, 3p13, 5q21 6q15.MSH6, was detectable 89.5%, 85.4% 85.0% cancers particularly strong advanced pathological tumor stage (P < 0.0001 each), high Gleason grade nodal...
The prognosis of head and neck squamous cell carcinoma (HNSCC) patients remains poor. identification high-risk subgroups is needed for the development custom-tailored therapies. expression cancer-testis antigens (CTAs) has been linked to a worse in other cancer types; however, their prognostic value HNSCC unclear because only few have examined data on CTA protein are sparse. A tissue microarray consisting tumor samples from 453 was evaluated proteins using immunohistochemistry. Frequency...