Elena Bady
A5066249474- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Radiomics and Machine Learning in Medical Imaging
- Immune cells in cancer
- Bladder and Urothelial Cancer Treatments
- Single-cell and spatial transcriptomics
- Cancer Research and Treatments
- Molecular Biology Techniques and Applications
- Peptidase Inhibition and Analysis
- Cancer Genomics and Diagnostics
- Prostate Cancer Treatment and Research
- Immune Cell Function and Interaction
- Cell Image Analysis Techniques
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Metastasis and carcinoma case studies
- Cancer Cells and Metastasis
- RNA modifications and cancer
- Melanoma and MAPK Pathways
- Colorectal Cancer Treatments and Studies
- Ferroptosis and cancer prognosis
- Prostate Cancer Diagnosis and Treatment
- vaccines and immunoinformatics approaches
- Cellular Mechanics and Interactions
- Urinary and Genital Oncology Studies
Universität Hamburg
2020-2025
University Medical Center Hamburg-Eppendorf
2020-2025
Universitätsklinikum Würzburg
2020-2024
University of North Carolina at Chapel Hill
2022
University of Nebraska Medical Center
2022
VA Western New York Healthcare System
2022
Pathologie Hamburg-West
2020
Introduction: GATA3 is a transcription factor involved in epithelial cell differentiation. immunostaining used as diagnostic marker for breast and urothelial cancer but can also occur other neoplasms. Methods: To evaluate normal tumor tissues, tissue microarray containing 16,557 samples from 131 different types subtypes 608 of 76 was analyzed by immunohistochemistry. Results: positivity found 69 including 23 (18%) with at least one strongly positive tumor. Highest rates occurred noninvasive...
The Ki-67 labeling index (Ki-67 LI) is a strong prognostic marker in prostate cancer, although its analysis requires cumbersome manual quantification of immunostaining 200-500 tumor cells. To enable automated LI assessment routine clinical practice, framework for quantification, which comprises three different artificial intelligence steps and an algorithm cell-distance multiplex fluorescence immunohistochemistry (mfIHC) staining, was developed validated cohort 12,475 cancers. impact the...
Programmed death ligand 1 (PD-L1) is the target of immune checkpoint inhibitor therapies in a growing number tumor types, but unanimous picture on PD-L1 expression across cancer types lacking.We analyzed immunohistochemical 11,838 samples from 118 human and its relationship with infiltrating CD8 positive lymphocytes.At cut-off level 10% cells, positivity was seen 85 (72%) including thymoma (100% positive), Hodgkin's lymphoma (93%), anaplastic thyroid carcinoma (76%), Kaposi sarcoma (71%),...
Quantity and the spatial relationship of specific immune cell types can provide prognostic information in bladder cancer. The objective study was to characterize interplay role different subpopulations
Abstract Microsatellite instability is a strong predictor of response to immune checkpoint therapy and patient outcome in colorectal cancer. Although enrichment distinct T‐cell subpopulations has been determined impact the outcome, little known about underlying changes composition tumor microenvironment. To assess density, composition, degree functional marker expression, spatial interplay subpopulations, 79 microsatellite instable (MSI) 1,045 stable (MSS) cancers were analyzed. A tissue...
Abstract Complete expression loss of S-methyl-5′-thioadenosine phosphorylase (MTAP) is caused by homozygous 9p21 deletion and results in a critical vulnerability cancer cells towards drugs targeting multiple different pathways. MTAP deficiency common urothelial cancer, but data on its intratumoral heterogeneity - potential obstacle for targeted therapies are lacking. To study the deletions advanced primary cancers urinary bladder, tissue microarray (TMA) was constructed from 5 sites blocks...
Abstract Glucose transporter 1 (GLUT1) is a key protein for transmembranous glucose uptake of cells which often overexpressed in cancer. To better comprehend the prevalence and role GLUT1 expression different cancer types, tissue microarray containing 14, 966 samples from 134 tumor entities was analyzed this study. generally markedly higher than corresponding normal tissues. A total 122 categories showed at least one case, 97 included case with strong staining. The frequency positivity...
CBP (CREB-binding protein) is a ubiquitously expressed multidomain protein with histone acetyltransferase and transcriptional co-activator activity. As major modifier, it involved in the regulation of thousands genes. has complex role cancer where both loss-of-function overexpression can occur. To better comprehend expression cancer, tissue microarray containing 14,966 samples from 134 different tumor entities 608 76 normal types was analyzed. In tissues, strong nuclear staining seen except...
Abstract KDM6A, also known as UTX (ubiquitously transcribed X chromosome tetratricopeptide repeat protein) is a critical epigenetic regulator which often subject to truncating mutations and subsequent expression loss in cancer. To interrogate the clinical significance of aberrant KDM6A expression, tissue microarray containing prostate cancers from patients treated by prostatectomy was analyzed immunohistochemistry. immunostaining weak 33.5%, moderate 52.9%, strong 7.3% 13, 747 interpretable...
Abstract Occludin is a key component of tight junctions. Reduced occludin expression has been linked to cancer progression. To study the role in cancer, tissue microarray containing 16, 870 samples from 148 different tumor types and 608 76 normal was analyzed by immunohistochemistry. immunostaining observed 10, 746 (76.6%) 14, 017 analyzable tumors, including 18.9% with weak, 16.2% moderate, 41.6% strong staining intensity. positivity found 134 categories most frequent adenocarcinomas...
Abstract Lung cancer is the most frequent tumor related reason for death worldwide. It hoped that a better understanding of immune environment can foster development new checkpoint therapies to improve patient management and survival. To study composition prognostic impact infiltrate in non-small cell lung cancer, tissue microarray containing 798 patients, including 441 adenocarcinomas, 219 squamous carcinomas, was analyzed by multiplex fluorescence immunohistochemistry. A set 14 antibodies...
Abstract Quantification of tumor infiltrating lymphocytes (TILs) and immune checkpoints in the cancer microenvironment has become increasing interested immuno-oncology. Vulvar is emerging as a promising target for checkpoint blockade therapy. However, only little known about presence TILs expression molecules its predictive value vulvar cancer. To better comprehend role cell composition cancer, tissue microarray containing 479 samples with clinic-pathological data was analyzed by multiplex...
Abstract STING is a key element in the cGAS/STING cytosolic DNA sensing pathway which can stimulate immune response through type I interferon (IFN) production. Numerous agonists are currently evaluated for their suitability as anticancer drugs. Most previous studies focused on expression inflammatory cells, but also be expressed cancer cells. To evaluate potential clinical significance of bladder was analyzed by immunohistochemistry more than 2,500 urothelial carcinomas tissue microarray...
Abstract TIM-3 (T-cell immunoglobulin and mucin-domain containing-3) is an inhibitory immune checkpoint molecule which mainly occurs in subsets of inflammatory cells. On the surface membrane cancer cells may represent a druggable target. To assess prevalence expression cancer, tissue microarray containing 17, 172 samples from 134 different tumor types subtypes was analyzed by immunohistochemistry (IHC). macrophages were semiquantitatively recorded. In cells, membranous positivity...
Abstract Loss of S-methyl-5′-thioadenosine phosphorylase (MTAP) expression offers an important therapeutic option through synthetic lethality with inhibitors targeting related pathways, and has been linked to resistance immune checkpoint in several tumor types. To better understand the prevalence clinical relevance MTAP prostate cancer, a tissue microarray 17, 747 cancer samples molecular database was analyzed for by immunohistochemistry. Normal glands showed weak moderate, predominantly...
Abstract KDM6A (lysine demethylase 6A) is an epigenetic regulator and part of the COMPASS-like complex. Truncating mutations commonly occur in cancer may result a dependency on EZH2, potential therapeutic target. Because only one copy X chromosome genes normally active human cells, truncating complete expression loss which can be seen by immunohistochemistry. To assess prevalence its role cancer, tissue microarray containing 14, 814 samples from 153 different tumor entities was analyzed. In...
Abstract 9p21 deletions including CDKN2A belong to the most common chromosomal aberrations in cancer. Most of topical interest comes S-methyl-5′-thioadenosine phosphorylase (MTAP) which is also included deletions. Homozygous are major cause for MTAP deficiency results a critical vulnerability cancer cells towards drugs targeting several different pathways. Fluorescence situ hybridization (FISH) gold standard method deletion detection because single always analyzed independent any admixture...
Abstract High-mobility group protein B1 (HMGB1) is a chromatin-associated with role in maintenance of nucleosome structure, transcription control, and DNA repair. Both its overexpression impaired function have been linked cancer development progression. To better comprehend the HMGB1 expression cancer, tissue microarray containing 14, 966 samples from 134 different tumor entities 608 76 normal types was analyzed. A strong nuclear staining occurred almost all cell most cancers. Of 11, 808...
The cell adhesions molecule L1 (CD171; L1CAM) was initially characterized as an essential protein for neural development but subsequent studies have pinpointed towards a critical role in cancer. Because of its expression only few normal tissues and membranous nature L1CAM is promising drug target. To better comprehend the different cancer types, tissue microarray containing 20,079 samples from 135 tumor entities 608 76 types analyzed. A staining found 1175 (9.1%) 12888 interpretable samples,...