A5008273506- Cancer Immunotherapy and Biomarkers
- Neuroendocrine Tumor Research Advances
- Cancer Cells and Metastasis
- Prostate Cancer Treatment and Research
- Immune cells in cancer
- Cancer and Skin Lesions
- Pancreatic and Hepatic Oncology Research
- Cancer Research and Treatments
- Single-cell and spatial transcriptomics
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Genetic factors in colorectal cancer
- HER2/EGFR in Cancer Research
- Monoclonal and Polyclonal Antibodies Research
- Ovarian cancer diagnosis and treatment
- Colorectal Cancer Treatments and Studies
- Cancer Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Skin and Cellular Biology Research
- Immune Cell Function and Interaction
- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Urinary and Genital Oncology Studies
- Cell Image Analysis Techniques
- Sarcoma Diagnosis and Treatment
University Medical Center Hamburg-Eppendorf
2012-2024
Universität Hamburg
2012-2024
University of Nebraska Medical Center
2022
University of North Carolina at Chapel Hill
2022
VA Western New York Healthcare System
2022
Klinikum Itzehoe
2021
Taipei Institute of Pathology
2021
Mesothelin (MSLN) represents an attractive molecule for targeted cancer therapies. To identify tumors that might benefit from such therapies, tissue microarrays including 15,050 122 different tumor types and 76 healthy organs were analyzed MSLN expression by immunohistochemistry. Sixty-six (54%) showed at least occasional weak staining, 50 (41%) with one strongly positive sample. Highest prevalence of positivity had ovarian carcinomas (serous 97%, clear cell 83%, endometrioid 77%, mucinous...
Trophoblast cell surface antigen 2 (TROP2) is the target of sacituzumab govitecan, an antibody-drug conjugate approved for treatment triple negative breast cancer and urothelial carcinoma.A tissue microarray containing 18,563 samples from 150 different tumor types subtypes as well 608 76 normal was analyzed by TROP2 immunohistochemistry.TROP2 positivity found in 109 categories, including squamous carcinomas various origins, urothelial, breast, prostate, pancreatic, ovarian cancers (>95%...
Introduction: GATA3 is a transcription factor involved in epithelial cell differentiation. immunostaining used as diagnostic marker for breast and urothelial cancer but can also occur other neoplasms. Methods: To evaluate normal tumor tissues, tissue microarray containing 16,557 samples from 131 different types subtypes 608 of 76 was analyzed by immunohistochemistry. Results: positivity found 69 including 23 (18%) with at least one strongly positive tumor. Highest rates occurred noninvasive...
TIGIT is an inhibitory immune checkpoint receptor and a putative target for novel therapies. Here, we analysed two different types of tissue microarrays healthy lymphatic various inflamed tissues, colorectal lung cancers, as well >1700 tumour samples from 86 entities and/or PD-1 by bright field multiplex fluorescence immunohistochemistry. was detected in CD8 + cytotoxic T cells, CD4 helper FOXP3 regulatory NK but not CD11c dendritic CD68 macrophages, CD20 B lymphocytes. expression...
Abstract Background Tumor protein 63 (p63) is a transcription factor of the p53 gene family involved in differentiation several tissues including squamous epithelium. p63 immunohistochemistry broadly used for tumor classification but published data on its expression cancer conflicting. Methods To comprehensively catalogue expression, tissue microarrays (TMAs) containing 12,620 samples from 115 entities and 76 normal types were analyzed. Results was seen various epithelium urothelium. At...
Abstract Background Cytokeratin 18 (CK18) is an intermediate filament protein of the cytokeratin acidic type I group and primarily expressed in single-layered or “simple” epithelial tissues carcinomas different origin. Methods To systematically determine CK18 expression normal cancerous tissues, 11,952 tumor samples from 115 types subtypes (including carcinomas, mesenchymal biphasic tumors) as well 608 76 tissue were analyzed by immunohistochemistry a microarray format. Results was cells...
Abstract Background The E-Cadherin gene ( CDH1, Cadherin 1 ), located at 16q22.1 encodes for a calcium-dependent membranous glycoprotein with an important role in cellular adhesion and polarity maintenance. Methods To systematically determine protein expression normal cancerous tissues, 14,637 tumor samples from 112 different types subtypes as well 608 of 76 tissue were analyzed by immunohistochemistry microarray format. Results was strongly expressed epithelial cells most organs. From 77...
Expansion of CD8+ cytotoxic Tlymphocytes is a prerequisite for anti-cancer immune activity and has gained interest in the era checkpoint therapy.To understand T cell dynamics tumor microenvironment, we used multiplex fluorescence immunohistochemistry to quantitate proliferation (Ki67 co-expression) tissue microarrays from 1107 colorectal, 642 renal cell, 1066 breast, 375 ovarian, 451 pancreatic 347 gastric cancer samples.The density percentage proliferating (Ki67+) cells were both highly...
Combined analysis of cytokeratin 7 (CK7) and 20 (CK20) is often used for assessing the origin metastatic cancer. To evaluate diagnostic utility CK7 CK20, tissue microarrays containing 15,424 samples from 120 different tumor types subtypes 608 76 normal were analyzed by immunohistochemistry. positivity was seen in 52% (8.7% weak, 5.9% moderate, 37% strong) CK20 23% (5.1% 3.4% 15% interpretable tumors. Of 8390 positive tumors, 1181 (14%) showed 5380 (64%) alone, 1829 (22%) alone. predominated...
To evaluate cytokeratin 19 (CK19) expression in normal and cancerous tissues, 15,977 samples from 122 tumor types 608 of 76 tissue were analyzed by immunohistochemistry (IHC). In CK19 occurred epithelial cells most glandular organs but was strictly limited to the basal cell layer nonkeratinizing squamous epithelium absent skin. ≥90% cases seen 34% entities including adenocarcinomas pancreas (99.4%), colorectum (99.8%), esophagus (98.7%), stomach (97.7%), as well breast cancer (90.0%-100%),...
p16 (CDKN2A) is a member of the INK4 class cell cycle inhibitors, which often dysregulated in cancer. However, prevalence expression different cancer types controversial. 15,783 samples from 124 tumor and 76 normal tissue were analyzed by immunohistochemistry microarray format. was detectable 5,292 (45.0%) 11,759 interpretable tumors. Except adenohypophysis islets Langerhans, staining largely absent tissues. In cancer, highest positivity rates observed uterine cervix squamous carcinomas...
Hodgkin's lymphoma (HL) is characterized by a high background of inflammatory cells which play an important role for the pathogenesis disease. T cell immunoreceptor with Ig and ITIM domains (TIGIT) inhibitory immune checkpoint receptor putative target novel immunotherapies. To study patterns TIGIT expression in surrounding malignant including Hodgkin cells, Reed-Sternberg histiocytic microenvironment (ME) tissue microarray (TMA) was constructed from punches measuring 2 mm diameter obtained...
Programmed death ligand 1 (PD-L1) is the target of immune checkpoint inhibitor therapies in a growing number tumor types, but unanimous picture on PD-L1 expression across cancer types lacking.We analyzed immunohistochemical 11,838 samples from 118 human and its relationship with infiltrating CD8 positive lymphocytes.At cut-off level 10% cells, positivity was seen 85 (72%) including thymoma (100% positive), Hodgkin's lymphoma (93%), anaplastic thyroid carcinoma (76%), Kaposi sarcoma (71%),...
BackgroundMultiple myeloma is a malignancy characterized by the expansion of plasma cell clone that localizes to human bone marrow. Myeloma cells and marrow stromal produce soluble factors promote survival progression multiple myeloma. Interleukin 16 (IL-16) involved in regulating migration proliferation normal leukocytes. However, role IL-16 cancers, including myeloma, unclear.
Carboxypeptidase A1 (CPA1) is a zinc metalloprotease that produced in pancreatic acinar cells and plays role cleaving C-terminal branched-chain aromatic amino acids from dietary proteins. This study assessed the utility of immunohistochemical CPA1 staining for diagnosing cell carcinoma (ACC). A total 12,274 tumor samples 132 different types subtypes as well 8 each 76 normal tissue were interpretable by immunohistochemistry microarray format. was strongly expressed all pancreas but not any...
Special AT-rich sequence-binding protein 2 (SATB2) induces local chromatin loops to facilitate transcription. SATB2 immunostaining is commonly used as a marker for colorectal adenocarcinoma and osteosarcoma.To extend our knowledge on the diagnostic value of analysis in comprehensive set human tumors.Tissue microarrays with 15 012 samples from 120 tumor types 608 76 different normal tissues were analyzed.SATB2 positivity was found 89 (74%), including 59 (49%) at least 1 moderately positive 38...
Human mammaglobin-A (SCGB2A2) is a secretory protein with an unknown function that used as diagnostic marker for breast cancer. However, other tumors can also express mammaglobin-A. To comprehensively study patterns of expression, tissue microarray containing 16,328 samples from 128 different tumor types well 608 76 normal was analyzed using immunohistochemistry. Mammaglobin-A positivity found in only few tissues, including luminal cells the endocervical and endometrial glands. In 37...
Background: Novel aspartic proteinase of the pepsin family A (Napsin A, TAO1/TAO2) is a functional which involved in maturation prosurfactant protein B type II pneumocytes and lysosomal catabolism renal cells. Napsin highly expressed adenocarcinomas lung thus commonly used to affirm this diagnosis. However, studies have shown that other tumors can also express A. Methods: To comprehensively determine expression normal tumor tissue, 11,957 samples from 115 different types subtypes as well 500...
Background: Mucin 5AC (MUC5AC) belongs to the glycoprotein family of secreted gel-forming mucins and is physiologically expressed in some epithelial cells. Studies have shown that MUC5AC also several cancer types suggesting a potential utility for distinction tumor subtypes. Methods: To systematically determine expression normal cancerous tissues, tissue microarray containing 10 399 samples from 111 different subtypes as well 608 76 was analyzed by immunohistochemistry. Results:...
Kallikrein-related peptidase 2 (KLK2)-like KLK3 (prostate-specific antigen [PSA])-belongs to the highly conserved serine proteases of glandular kallikrein protein family (KLK family). Studies suggested that measurement KLK2 serum levels advanced predictive accuracy PSA testing in prostate cancer.To clarify potential utility as a prognostic tissue biomarker, expression was analyzed by immunohistochemistry more than 12 000 cancers.Normal epithelium cells usually showed weak moderate...
Thyroglobulin is a secreted 660 kDa glycoprotein produced by thyroid follicular cells used in diagnostic pathology to secure or exclude thyroidal origin of metastases unknown primary tumors. This study was performed estimate specificity thyroglobulin immunohistochemistry. 9974 tumor samples from 109 different types and subtypes as well 608 76 normal tissue were analyzed immunohistochemistry microarray format. strongly expressed all but not any other tissues. immunostaining detected 99.1% 106...
The expression of the neuroendocrine markers synaptophysin and chromogranin A was analyzed by immunohistochemistry in 14,584 samples from 103 different tumor types subtypes a tissue microarray format. At least one these found to be positive 96.7% tumors various neoplasms. In non-neuroendocrine tumors, and/or staining seen 6.3% (n = 584), specifically 41 88 entities. Basal cell carcinomas skin (50% for alone) adrenocortical (91.7% stood out due frequent only specific marker. subdivision...