A5036307539- Cancer Immunotherapy and Biomarkers
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Cell Image Analysis Techniques
- Immunotherapy and Immune Responses
- Sarcoma Diagnosis and Treatment
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Virus-based gene therapy research
- Antifungal resistance and susceptibility
- Molecular Biology Techniques and Applications
- Cancer Research and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pneumonia and Respiratory Infections
- Metastasis and carcinoma case studies
- Glycosylation and Glycoproteins Research
- Cardiac tumors and thrombi
- Medical Imaging and Pathology Studies
- Cancer, Hypoxia, and Metabolism
- HER2/EGFR in Cancer Research
- COVID-19 and healthcare impacts
- Ovarian cancer diagnosis and treatment
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Monoclonal and Polyclonal Antibodies Research
- Neuroendocrine Tumor Research Advances
University Medical Center Hamburg-Eppendorf
2012-2025
Universität Hamburg
2012-2025
Eppendorf (Germany)
2025
Marienkrankenhaus Hamburg
2021
Zimmer Biomet (United Kingdom)
2021
Cadherin-17 (CDH17) is a membranous cell adhesion protein predominantly expressed in intestinal epithelial cells. CDH17 therefore considered possible diagnostic and therapeutic target. This study was to comprehensively determine the expression of cancer further assess utility immunohistochemistry (IHC). A tissue microarray containing 14,948 interpretable samples from 150 different tumor types subtypes as well 76 normal analyzed by IHC. In tissues, staining seen epithelium intestine...
Stimulator of interferon genes protein (STING) activates the immune response in inflammatory cells. STING expression cancer cells is less well characterized, but agonists are currently being evaluated as anticancer drugs. A tissue microarray containing 18,001 samples from 139 different tumor types was analyzed for by immunohistochemistry. STING-positive were found 130 (93.5%) entities. The highest positivity rates occurred squamous cell carcinomas (up to 96%); malignant mesothelioma...
ABSTRACT Background Obtaining non‐directed samples from the upper bronchial tree is easier to perform and poses fewer risks for critically ill patients than deep bronchoalveolar lavage (BAL). Since invasive pulmonary aspergillosis associated with a high mortality in patients, timely diagnosis rapid initiation of treatment are utmost importance. Objectives The objective this study was compare Galactomannan (GM) testing by Enzyme Immunoassay (EIA), GM Lateral Flow Assay (LFA) detection...
Anti-MDA5 positive dermatomyositis is a rare subtype of idiopathic inflammatory myopathies often accompanied by rapidly progressive interstitial lung disease and severe cutaneous ulcerations. It associated with high mortality. The simultaneous occurrence sarcoidosis multisystemic involvement myositis has only been described very rarely in the literature to date. We report case 39-year-old man 3-year history anti-MDA‑5 who developed worsening dyspnea hypercalcemia acute kidney injury marked...
Occludin is a key component of tight junctions. Reduced occludin expression has been linked to cancer progression in individual tumor types, but comprehensive and standardized analysis across human types lacking. To study the prevalence clinical relevance cancer, tissue microarray containing 16,870 samples from 148 different 608 76 normal was analyzed by immunohistochemistry. immunostaining observed 10,746 (76.6%) 14,017 analyzable tumors, including 18.9% with weak, 16.2% moderate, 41.6%...
EpCAM is expressed in many epithelial tumors and used for the distinction of malignant mesotheliomas from adenocarcinomas as a surrogate pan-epithelial marker. A tissue microarray containing 14,832 samples 120 different tumor categories was analyzed by immunohistochemistry. staining compared with TROP2 CKpan. detectable 99 categories. Among 78 types, positivity rate ≥90% 60 categories—including adenocarcinomas, neuroendocrine neoplasms, germ cell tumors. lowest hepatocellular carcinomas,...
PAX8 plays a role in development of the thyroid, kidney, and Wolffian Mullerian tract. In surgical pathology, immunohistochemistry is used to determine tumors renal ovarian origin, but data on its expression other are conflicting. To evaluate normal tumor tissues, tissue microarray containing 17,386 samples from 149 different types 608 76 was analyzed by immunohistochemistry. results were compared with previously collected cadherin 16 (CDH16). positivity found 40 types. The highest rate...
Histopathological changes in buccal mucosa transplants after engraftment to the urethra and exposure urine remain nebulous. We investigated histopathological integrated into humans.We prospectively evaluated 22 patients with recurrent urethral stricture urethroplasty between November 2012 October 2013. All underwent repeat performed by a single surgeon. Intraoperatively we harvested sample of transplant previously engrafted urethra, healthy freshly from contralateral inner cheek fibrotic...
Abstract Multiplex fluorescence IHC (mfIHC) approaches were yet either limited to six markers or a small tissue size that hampers translational studies on large microarray cohorts. Here we have developed BLEACH&STAIN mfIHC method enabled the simultaneous analysis of 15 biomarkers (PD-L1, PD-1, CTLA-4, panCK, CD68, CD163, CD11c, iNOS, CD3, CD8, CD4, FOXP3, CD20, Ki67, and CD31) in 3,098 tumor samples from 44 different carcinoma entities within one week. To facilitate automated immune...
Carcinoembryonic antigen (CEA) is a cell-surface glycoprotein serving as drug target, diagnostic marker, and serum marker for cancer monitoring. However, prevalence data on CEA expression in tissues vary considerably. This study was designed to determine normal neoplastic tissues.
Estrogen receptor (ER) expression is a prognostic parameter in breast cancer, and prerequisite for the use of endocrine therapy. In ER+ early however, no receptor-associated biomarker exists that identifies patients with particularly favorable outcome. We have investigated value ESR1 amplification predicting long-term clinical outcome tamoxifen-treated postmenopausal women endocrine-responsive cancer.394 who had been randomized into tamoxifen-only arm prospective ABCSG-06 trial adjuvant...
In the absence of lung biopsy, there are various algorithms for diagnosis invasive pulmonary aspergillosis (IPA) in critically ill patients that rely on clinical signs, underlying conditions, radiological features and mycology. The aim present study was to compare four diagnostic their ability differentiate between probable IPA (i.e., requiring treatment) colonisation. For this accuracy study, we included a mixed ICU population with positive Aspergillus culture from respiratory secretions...
Abstract Background Kallikrein-related peptidase 7 (KLK7) is a chymotrypsin-like serine protease which essential for the desquamation of corneocytes and thus plays pivotal role in maintaining skin homeostasis. In cancer, KLK7 overexpression was suggested to represent route metastasis through cleavage cell junction extracellular matrix proteins cancer cells. Methods To comprehensively determine protein expression normal neoplastic tissues, tissue microarray containing 13,447 samples from 147...
Loss of S-methyl-5′-thioadenosine phosphorylase (MTAP) expression is a common event in cancer leading to critical vulnerability cells towards anti-cancer drugs. Homozygous MTAP deletions result complete loss that can be detected by immunohistochemistry (IHC). In this study, tissue microarray containing 17,078 samples from 149 different tumor entities was analyzed IHC, and validated fluorescence situ hybridization. observed 83 categories, including neuroendocrine neoplasms (up 80%), Hodgkin...
Background/Objectives: Trefoil factor 1 (TFF1) plays a role in the mucus barrier. Methods: To evaluate prevalence of TFF1 expression cancer, tissue microarray containing 18,878 samples from 149 tumor types and 608 76 normal was analyzed through immunohistochemistry (IHC). Results: staining detectable 65 categories. The highest rates positivity were found mucinous ovarian carcinomas (76.2%), colorectal adenomas adenocarcinomas (47.1–75%), breast neoplasms (up to 72.9%), bilio-pancreatic...
Abstract In pandemics or to further study highly contagious infectious diseases, new strategies are needed for the collection of post-mortem tissue samples identify pathogen as well its morphological impact. this study, an ultrasound-guided minimally invasive sampling (MITS) protocol was developed and validated use. The histological microbiological qualities specimens were evaluated compared between MITS conventional autopsy (CA) in a series COVID-19 deaths. Thirty-six performed. five cases...
Abstract The current pandemic with Severe acute respiratory syndrome-coronavirus-2 has been taking on new dynamics since the emergence of variants last fall, some them spreading more rapidly. Many countries currently find themselves in a race to ramp up vaccination strategies that have initiated and possible third wave from variants, such as Variant Concern-202012/01 B.1.1.7 lineage. Until today, many investigations death cases Coronavirus-disease-19 conducted, revealing pulmonary damage be...
Zusammenfassung Das Angiosarkom ist eine seltene, maligne Erkrankung, die von den Blutgefäßen ausgeht. pulmonale kann sich diagnostisch initial wie chronisch thromboembolische Hypertonie (CTEPH) darstellen. Die CTEPH Erhöhung des pulmonal-arteriellen Drucks und Widerstands als Folge einer persistierenden Obstruktion der Lungenstrombahn nach pulmonalen Thromboembolien trotz adäquater Antikoagulation. In unserer Ambulanz für stellte ein 76-jähriger Patient mit anamnestisch vor 7 Monaten...
Abstract KDM6A, also known as UTX (ubiquitously transcribed X chromosome tetratricopeptide repeat protein) is an epigenetic regulator constituting a critical part of the COMPASS-like complex. KDM6A mutations occur commonly in cancer. These are interest because loss results dependency on EZH2, potential therapeutic target. Most data have been obtained by next generation sequencing (NGS). However, since most truncating and located chromosome, many result complete expression which can be...
Abstract The S-methyl-5′-thioadenosine phosphorylase (MTAP) gene is located at 9p21 and often co-deleted with CDKN2A. Because homozygous MTAP deletions result in a complete expression loss, immunohistochemistry (IHC) can be used for detection of deleted cells. IHC diagnostic utility the distinction mesotheliomas (often deleted) from benign mesothelial proliferations. Moreover, evidence emerging that deficiency results critical vulnerability cancer cells towards drugs targeting several...
The Melan-A (melanocyte antigen) protein, also termed 'melanoma antigen recognized by T cells 1' (MART-1) is a protein with unknown function whose expression specific for the melanocyte lineage. Antibodies against are thus used identifying melanocytic tumors, but some antibodies show an additional - diagnostically useful cross-reactivity unspecified involved in corticosteroid hormone synthesis. To comprehensively compare staining patterns of and cross-reactive antibody normal neoplastic...