Jan Kempski
A5043715050- Cancer Immunotherapy and Biomarkers
- Liver Disease Diagnosis and Treatment
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Inflammatory Bowel Disease
- Liver Diseases and Immunity
- Psoriasis: Treatment and Pathogenesis
- Esophageal Cancer Research and Treatment
- T-cell and B-cell Immunology
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Microscopic Colitis
- COVID-19 Clinical Research Studies
- Liver Disease and Transplantation
- Pancreatitis Pathology and Treatment
- Immune cells in cancer
- Long-Term Effects of COVID-19
- Ion Channels and Receptors
- Bioactive Compounds and Antitumor Agents
- Listeria monocytogenes in Food Safety
- Hepatitis Viruses Studies and Epidemiology
- Viral gastroenteritis research and epidemiology
- CAR-T cell therapy research
- Hepatocellular Carcinoma Treatment and Prognosis
Universität Hamburg
2015-2025
University Medical Center Hamburg-Eppendorf
2015-2025
Eppendorf (Belgium)
2024
University Cancer Center Hamburg
2023
The liver is one of the organs most commonly affected by metastasis. presence metastases has been reported to be responsible for an immunosuppressive microenvironment and diminished immunotherapy efficacy. Herein, we aimed investigate role IL-10 in metastasis determine how its modulation could affect efficacy vivo.
Intestinal inflammation can impair mucosal healing, thereby establishing a vicious cycle leading to chronic inflammatory bowel disease (IBD). However, the signaling networks driving remain unclear. Here we report that CD4+ T cells isolated from patients with IBD produce high levels of interleukin-22 binding protein (IL-22BP), endogenous inhibitor tissue-protective cytokine IL-22. Using mouse models, demonstrate development requires cell-derived IL-22BP. Lastly, intestinal responsive...
IL-22 has dual functions during tumorigenesis. Short term production protects against genotoxic stress, whereas uncontrolled activity promotes tumor growth; therefore, tight regulation of is essential. TGF-β1 the differentiation Th17 cells, which are known to be a major source IL-22, but effect TGF-β signaling on in CD4+ T cells controversial. Here we show an increased presence IL-17+IL-22+ and colorectal cancer compared normal adjacent tissue, frequency single producing not changed....
IL-10 is essential to maintain intestinal homeostasis. CD4+ T regulatory type 1 (TR1) cells produce large amounts of this cytokine and are therefore currently being examined in clinical trials as cell therapy patients with inflammatory bowel disease. However, factors molecular signals sustaining TR1 activity still need be identified optimize the efficiency ensure safety these trials. We investigated role signaling mature vivo. Double IL-10eGFP Foxp3mRFP reporter mice transgenic impairment...
During metastasis, cancer cells invade, intravasate, enter the circulation, extravasate, and colonize target organs. Here, we examined role of interleukin (IL)-22 in metastasis. Immune cell-derived IL-22 acts on epithelial tissues, promoting regeneration healing upon tissue damage, but it is also associated with malignancy. Il22-deficient mice treated an antibody were protected from colon-cancer-derived liver lung metastasis formation, while overexpression promoted Mechanistically, acted...
High-resolution imaging of live T cells characterizes the early Ca 2+ signals required for cell activation.
Objective There is a strong clinical association between IBD and primary sclerosing cholangitis (PSC), chronic disease of the liver characterised by biliary inflammation that leads to strictures fibrosis. Approximately 60%�80% people with PSC will also develop (PSC-IBD). One hypothesis explaining this would be drives IBD. Therefore, our aim was test decipher underlying mechanism. Design Colitis severity analysed in experimental mouse models colitis cholangitis, PSC-IBD. Foxp3 + Treg-cell...
Abstract Purpose Listeria monocytogenes causes severe bacterial infections with the highest mortality rate among foodborne pathogens in Europe. Combination treatment ampicillin and gentamicin is recommended for invasive manifestations. However, evidence to support this approach remains limited due a lack of randomised controlled trials. To explore critical issue further, we conducted retrospective, single-center study. Methods We identified all patients hospitalized listeriosis at University...
Abstract Acute liver injury can be secondary to a variety of causes, including infections, intoxication, and ischemia. All these insults induce hepatocyte death subsequent inflammation, which make acute life-threatening event. IL-22 is dual natured cytokine has context-dependent protective pathogenic properties during tissue damage. Accordingly, was shown promote regeneration upon However, other studies suggest chronic injury. binding protein (IL-22BP, IL-22Ra2) soluble inhibitor that...
Background & AimsUnregulated activity of interleukin (IL) 22 promotes intestinal tumorigenesis in mice. IL22 binds the antagonist subunit alpha 2 (IL22RA2, also called IL22BP). We studied whether alterations IL22BP contribute to colorectal carcinogenesis humans and mice.MethodsWe obtained tumor nontumor tissues from patients with cancer (CRC) measured levels cytokines by quantitative polymerase chain reaction, flow cytometry, immunohistochemistry. Il22bp messenger RNA colon wild-type,...
Primary sclerosing cholangitis is one of the most challenging conditions in hepatology, and due to our limited understanding its pathogenesis, no causal therapies are currently available. While it was long assumed that a minority people with IBD also develop PSC, which sometimes labeled an extraintestinal manifestation IBD, clinical phenotype, genetic intestinal microbiota associations strongly argue for PSC-IBD being distinct form existing alongside ulcerative colitis Crohn’s disease. In...
Abstract Background and aims Hepatitis E virus (HEV), similar to hepatitis A (HAV), has been linked cases associated with mussel consumption, several studies have detected HEV in commercially available mussels. While extensive data exists on contamination mussels from tropical regions, the Mediterranean Sea, North there is a lack of information regarding potential risk posed by common ( Mytilidae ) Baltic Sea. Furthermore, no experimental investigated ability Sea be infected HEV. Material...
Abstract Altered O -glycosylation is a key contributor to various pathophysiological processes. Notably, the expression of Tn antigen primarily attributed dysfunction chaperone Cosmc, while overexpression polypeptide N -acetylgalactosaminyltransferases (GalNAc-Ts) has also been implicated in numerous diseases. We generated transgenic mouse model with conditional Cosmc- knockout and simultaneous -acetylgalactosaminyltransferase 2 (GalNT2) mediated by pancreas-specific transcription factor 1a...
Metastasis is a cancer-related systemic disease and responsible for the greatest mortality rate among cancer patients. Interestingly, interaction between immune system cells seems to play key role in metastasis formation target organ. However, this complex network only partially understood. We previously found that IL-22 produced by tissue resident iNKT17 promotes cell extravasation, early step of metastasis. Based on these data, we aimed here decipher last formation. levels were increased...
Hepatocellular carcinoma (HCC) ranks among the five most common cancer entities worldwide and leads to hundred-thousands of deaths every year. Despite some groundbreaking therapeutical revelations during last years, overall prognosis remains poor. Although immune system fights malignant transformations with a robust anti-tumor response, certain mediators have also been shown promote development. For example, interleukin (IL)-22 has associated HCC progression worsened in multiple studies....
Abstract Background Primary sclerosing cholangitis (PSC) is a progressive liver disease associated with inflammatory bowel (IBD). The percentage of PSC patients diagnosed concomitant IBD varies considerably between studies. This raises the question whether all would show intestinal inflammation if screened thoroughly, even in absence symptoms. Methods To address this question, we collected biopsies healthy controls (n = 34), 25), PSC-IBD 41), and 51) cross-sectional study carried out...
The intestine is constantly balancing the maintenance of a homeostatic microbiome and protection host against pathogens such as viruses. Many cytokines mediate protective inflammatory responses in intestine, among them IL-1β. IL-1β proinflammatory cytokine typically activated upon specific danger signals sensed by inflammasome. SARS-CoV-2 capable infecting multiple organs, including intestinal tract. Severe cases COVID-19 were shown to be associated with dysregulated immune response,...
Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This virus does not only lead to pulmonary infection but can also infect other organs such as gut, kidney, or liver. Recent studies confirmed that cases of COVID-19 are often associated with liver damage and failure, well systemic upregulation pro-inflammatory cytokines tumor necrosis factor-alpha (TNFα). However, impact these immune mediators in have on patient survival during...
Reflux promotes esophageal adenocarcinomas (EACs) creating a chronic inflammatory environment. Survival rates are low due to early local recurrences and distant metastasis. Hence, there is need for new potential treatment options like immunotherapies. However, the microenvironment in EACs its impact on patient outcome remain be fully understood.mRNA expression levels of pro- anti-inflammatory markers 39 EAC patients without neoadjuvant radio-chemotherapy were measured. Data confirmed using...
BackgroundInsufficient remnant liver volume (RLV) after the resection of hepatic malignancy could lead to failure and mortality. Portal vein ligation (PVL) prior hepatectomy is subsequently introduced increase improve outcome malignancy. IL-22 has previously been reported promote regeneration, while facilitating tumor development in via Steap4 upregulation. Here we performed PVL mouse models study role regeneration post-PVL.MethodsLiver weight was measured magnetic resonance imaging (MRI)....
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which is responsible for eliciting Coronavirus disease 2019 (COVID-19) still challenges healthcare services worldwide. While many patients only suffer from mild symptoms, with some pre-existing medical conditions are at a higher risk detrimental course of disease. However, the underlying mechanisms determining partially understood. One key factor influencing severity described to be immune-mediated. In this report, we describe...
Background Primary sclerosing cholangitis (PSC) is a chronic liver disease marked by inflammation of the bile ducts and results in development strictures fibrosis. A robust clinical correlation exists between PSC inflammatory bowel (IBD). At present, published data are controversial, it yet unclear whether IBD drives or attenuates PSC. Methods Mdr2 -deficient mice DDC-fed were used as experimental models for cholangitis. Additionally, colitis was induced with cholangitis, either through...