A5003877367- Gut microbiota and health
- Diet and metabolism studies
- Digestive system and related health
- Epigenetics and DNA Methylation
- Erythrocyte Function and Pathophysiology
- Neonatal Health and Biochemistry
- Immune Cell Function and Interaction
- Inflammatory Bowel Disease
- Immune cells in cancer
- Pancreatic function and diabetes
- Blood properties and coagulation
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Ion channel regulation and function
- Dietary Effects on Health
- Hemoglobinopathies and Related Disorders
- Pancreatic and Hepatic Oncology Research
- Cancer Immunotherapy and Biomarkers
- RNA regulation and disease
- Cancer and Skin Lesions
- Vascular Malformations and Hemangiomas
- Genetic and rare skin diseases.
- Infant Nutrition and Health
- Endoplasmic Reticulum Stress and Disease
- Immunodeficiency and Autoimmune Disorders
University Hospital Schleswig-Holstein
2019-2025
University of Lübeck
2019-2025
Kiel University
2018-2025
Nova Southeastern University
2025
Universität Hamburg
2023-2025
University Medical Center Hamburg-Eppendorf
2023-2025
University of Tübingen
2015
University of Messina
2015
University of Catania
2000
The liver is one of the organs most commonly affected by metastasis. presence metastases has been reported to be responsible for an immunosuppressive microenvironment and diminished immunotherapy efficacy. Herein, we aimed investigate role IL-10 in metastasis determine how its modulation could affect efficacy vivo.
Abstract Background The interplay of epigenetic processes and the intestinal microbiota may play an important role in development homeostasis. Previous studies have established that regulates a large proportion epithelial transcriptome adult host, but microbial effects on DNA methylation gene expression during early postnatal are still poorly understood. Here, we sought to investigate cells (IECs) development. Methods We collected IECs from small intestine each five 1-, 4- 12 16-week-old...
Excess and unresolved endoplasmic reticulum (ER) stress in intestinal epithelial cells (IECs) promotes inflammation. Activating transcription factor 6 (ATF6) is one of the signaling mediators ER stress. We studied pathways that regulate ATF6 its role for inflammation IECs.We performed an RNA interference screen, using 23,349 unique small interfering RNAs targeting 7783 genes a luciferase reporter controlled by ATF6-dependent ERSE (ER stress-response element) promoter, to identify proteins...
Objective One of the current hypotheses to explain proinflammatory immune response in IBD is a dysregulated T cell reaction yet unknown intestinal antigens. As such, it may be possible identify disease-associated clonotypes by analysing peripheral and T-cell receptor (TCR) repertoire patients with controls. Design We performed bulk TCR profiling both alpha beta chains using high-throughput sequencing blood samples total 244 healthy controls as well from matched tissue 59 disease further...
Background/Aims: The JAK1/JAK2 tyrosine kinase inhibitor ruxolitinib is widely used for the treatment of myeloproliferative neoplasm-associated myelofibrosis and other malignancies. Most important side effects include anemia. A common cause anemia accelerated suicidal death erythrocytes or eryptosis, which characterized by cell shrinkage membrane scrambling with phosphatidylserine translocation to erythrocyte surface. Mechanisms contributing triggering eryptosis oxidative stress, Ca2+ entry...
Background/Aims: The benzophenone garcinol from dried fruit rind of Garcinia indica counteracts malignancy, an effect at least in part due to stimulation apoptosis. proapototic is attributed inhibition histone acetyltransferases and thus modification gene expression. Moreover, triggers mitochondrial depolarisation. Erythrocytes lack expression mitochondria but are nevertheless able enter apoptosis-like suicidal death or eryptosis, which characterized by cell shrinkage membrane scrambling...
Background/Aims: The anti-inflammatory, anti-autoimmune, antiparasitic, and anti-viral ether phospholipid edelfosine (1-O-octadecyl-2-O-methylglycero-3-phosphocholine) stimulates apoptosis of tumor cells is thus considered for the treatment malignancy. Similar to nucleated cells, erythrocytes may enter eryptosis, suicidal erythrocyte death characterized by cell shrinkage scrambling membrane with phosphatidylserine translocation surface. Triggers eryptosis include Ca2+ entry increase...
Article23 September 2019Open Access Source DataTransparent process Prdx4 limits caspase-1 activation and restricts inflammasome-mediated signaling by extracellular vesicles Simone Lipinski Corresponding Author [email protected] orcid.org/0000-0002-9322-7372 Institute of Clinical Molecular Biology, Christian-Albrechts-University University Hospital Schleswig-Holstein, Campus Kiel, Germany Search for more papers this author Steffen Pfeuffer Philipp Arnold Anatomical Institute, Christian Treitz...
Genetic variants in the DNA methyltransferase 3 A (DNMT3A) locus have been associated with inflammatory bowel disease (IBD). DNMT3A is part of epigenetic machinery physiologically involved methylation. We show that plays a critical role maintaining intestinal homeostasis and gut barrier function. expression downregulated epithelial cells from IBD patients upon tumor necrosis factor treatment murine organoids. Ablation Caco-2 results global hypomethylation, which linked to impaired...
Background: The P-glycoprotein inhibitor zosuquidar (LY335979) is clinically used to augment the effect of cytostatic drugs on suicidal tumor cell death or apoptosis. present study explored whether substance cytotoxic erythrocytes. Upon injury, erythrocytes may undergo eryptosis, which characterized by shrinkage and translocation membrane phosphatidylserine erythrocyte surface. Signaling eryptosis include increase cytosolic Ca2+-activity ([Ca2+]i), oxidative stress activation several...
Background/Aims: The alkylating drug oxaliplatin is widely used for chemotherapy of malignancy. Oxaliplatin effective by inducing both, necrosis and apoptosis. Similar to or apoptosis nucleated cells, erythrocytes may enter hemolysis, which apparent from hemoglobin release eryptosis, characterized cell shrinkage membrane scrambling with phosphatidylserine translocation the erythrocyte surface. Triggers eryptosis include oxidative stress and/or Ca2+ entry increase cytosolic activity...
This manuscript reports intraocular IgG4-related disease (IgG4-RD) presenting as panuveitis with a large subretinal mass and contralateral orbital mass. It includes literature review highlighting IgG4-RD's diagnostic challenges, emphasizing clinical, histopathologic, multimodal-imaging findings. PubMed was searched for "all fields" keywords "IgG4" AND "retina" "choroid" from 1974 to March 2024, revealing 16 cases of 23 eyes detailing chorioretinal manifestations. The most common symptom...
Abstract Background Alterations in the composition and function of intestinal microbiota have been observed organismal aging across a broad spectrum animal phyla. Recent findings, which derived mostly simple models, even established causal relationship between age-related microbial shifts lifespan, suggesting microbiota-directed interventions as potential tool to decelerate processes. To test whether life-long microbiome rejuvenation strategy could delay or prevent non-ruminant mammals, we...
The natural phosphoprotein phosphatase inhibitor cantharidin, primarily used for topical treatment of warts, has later been shown to trigger tumor cell apoptosis and is thus considered the malignancy. Similar cells, erythrocytes may undergo eryptosis, a suicidal death characterized by shrinkage translocation membrane phosphatidylserine erythrocyte surface. Signaling eryptosis includes increase cytosolic Ca2+-activity ([Ca2+]i), ceramide, oxidative stress dysregulation several kinases....
Abstract Background Genetic variants in the de novo DNA methyltransferase DNMT3A associate with increased risk for inflammatory bowel disease (IBD). While loss-of-function somatic mutations have been attributed to myelodysplasia and acute myeloid leukemia, exact mechanisms how defective is linked IBD remain elusive. Since macrophages are key gatekeepers immune homeostasis, we aimed characterize loss affects monocytic lineage differentiation properties. Methods We generated mice lacking...
AbstractHailey-Hailey disease is a rare autosomal dominant acantholytic disorder characterized from late adolescence or adulthood by recurrent eruptions of vesicles and blisters usually located on the neck, axillae groin. The clinical cases four unrelated adult male patients with Hailey-Hailey are presented. In all patients, relatively short-course treatment oral erythromycin (3-4 weeks) induced longlasting remission (8 months). Since bacteriological investigations excluded local infection,...
Summary Hexokinases (HK) catalyze the first step of glycolysis and thereby limit its pace. HK2 is highly expressed in gut epithelium, plays a role immune responses upregulated inflammation ulcerative colitis 1–3 . Here, we examined microbial regulation impact on intestinal by generating mice lacking specifically epithelial cells ( Hk2 Δ IEC ). were less susceptible to acute upon challenge with dextran sodium sulfate (DSS). Analyzing transcriptome from during revealed downregulation cell...