A5073762729- Gut microbiota and health
- Diet and metabolism studies
- Metabolomics and Mass Spectrometry Studies
- Trace Elements in Health
- Heavy Metal Exposure and Toxicity
- Probiotics and Fermented Foods
- Tryptophan and brain disorders
- Infant Nutrition and Health
- Digestive system and related health
- Microbial Metabolic Engineering and Bioproduction
- Electrochemical Analysis and Applications
- Clostridium difficile and Clostridium perfringens research
- Immune Cell Function and Interaction
- Analytical Chemistry and Chromatography
- Dietary Effects on Health
- Pancreatic function and diabetes
- Biochemical Analysis and Sensing Techniques
- Epigenetics and DNA Methylation
- Plant-Microbe Interactions and Immunity
- IL-33, ST2, and ILC Pathways
- Agricultural Productivity and Crop Improvement
- Salivary Gland Disorders and Functions
- Advanced Proteomics Techniques and Applications
- Medicinal Plants and Neuroprotection
- Inflammatory Bowel Disease
Helmholtz Zentrum München
2015-2025
Center for Environmental Health
2016
German Center for Diabetes Research
2012-2014
Technical University of Munich
2011-2013
Leibniz-Institute for Food Systems Biology at the Technical University of Munich
2011
<h3>Objective</h3> Iron deficiency is a common complication in patients with IBD and oral iron therapy suggested to exacerbate symptoms. We performed an open-labelled clinical trial compare the effects of per (PO) versus intravenous (IV) replacement (IRT). <h3>Design</h3> The study population included Crohn9s disease (CD; N=31), UC (N=22) control subjects (non-inflamed, NI=19). After randomisation, participants received sulfate or sucrose over 3 months. Clinical parameters, faecal bacterial...
Short-chain fatty acids (SCFAs), which are generated by the bacterial fermentation of dietary fibers, promote expansion regulatory T cells (Tregs). Potential therapeutic value SCFAs has been recently highlighted in experimental models cell-mediated autoimmunity and allergic inflammation. These studies suggest that physiological intestinal concentrations within millimolar range crucial for dampening inflammation-mediated processes. Here, we describe opposing effects on immune responses. In...
Altered interactions between the mucosal immune system and intestinal microbiota contribute to pathogenesis of inflammatory bowel diseases (IBD). It is not clear how inhibitors cytokines, such as antagonists tumor necrosis factor (anti-TNF), affect microbiome. We investigated effects anti-TNF agents on gut microbe community structure function in a longitudinal 2-step study patients with IBD. correlated our findings outcomes treatment patterns metabolites fecal samples before after therapy.We...
Gut microbiota may promote positive energy balance; however, germfree mice can be either resistant or susceptible to diet-induced obesity (DIO) depending on the type of dietary intervention. We here sought identify constituents that determine susceptibility body fat accretion in (GF) mice. GF and specific pathogen free (SPF) male C57BL/6N were fed high-fat diets based lard palm oil for 4 wks. Mice metabolically characterized at end feeding trial. FT-ICR-MS UPLC-TOF-MS used cecal as well...
Abstract Background The rhizosheath, a cohesive soil layer firmly adhering to plant roots, plays vital role in facilitating water and mineral uptake. In pearl millet, rhizosheath formation is genetically controlled influenced by root exudates. Here, we investigated the impact of exudates on microbiota composition, interactions, assembly processes, structure millet using four distinct lines with contrasting aggregation abilities. Results Utilizing 16S rRNA gene ITS metabarcoding for...
The gut microbiota is an important influencing factor of metabolic health. Although dietary interventions with probiotics, prebiotics, and synbiotics can be effective means to regulate obesity associated comorbidities, the underlying shifts in microbial communities, especially at functional level, have not been characterized great details. In this study, we sought investigate effects on regulation alleviation high-fat diet (HFD)-induced disorders mice. Specific pathogen-free (SPF) male...
The gut microbiota generates a huge pool of unknown metabolites, and their identification characterization is key challenge in metabolomics. However, there are still gaps on the studies chemical structures. In this investigation, an unusual class bacterial sulfonolipids (SLs) detected mouse cecum, which was originally found environmental microbes. We have performed detailed molecular level lipids by combining high-resolution mass spectrometry liquid chromatography analysis. Eighteen SLs that...
Occupationally or environmentally caused chronic exposure to Manganese (Mn) can lead a degeneration of dopaminergic neurons inducing Parkinson-like complaint called manganism. Deciphering the ongoing neurodegenerative mechanisms in affected brain is still major task for understanding complex modes action. Therefore, we applied non-toxic, oral feeding rats simulating Mn. Analysis extracts by electrospray ionization Fourier transform resonance mass spectrometry (ESI-FT-ICR-MS) revealed an...
The autoimmune neurological disease, Multiple Sclerosis (MS), have increased at alarming rates in the Western society over last few decades. While there are numerous efforts to develop novel treatment approaches, is an unmet need identify preventive strategies. We explored whether central nervous system (CNS) autoimmunity can be prevented through dietary manipulation using a spontaneous encephalomyelitis mouse model. report that nutritional supplementation of non-fermentable fiber, common...
Although it is generally accepted that dietary fiber health promoting, the underlying immunological and molecular mechanisms are not well defined, especially with respect to cellulose, most ubiquitous fiber. Here, impact of cellulose on intestinal microbiota, immune responses gene expression in disease was examined. Lack disrupted age-related diversification which subsequently remained an immature state. Interestingly, one affected microbial genera Alistipes equipped enzymes degrade...
The early-life metabolome of the intestinal tract is dynamically influenced by colonization gut microbiota which in turn affected nutrition, i.e. breast milk or formula. A detailed examination fecal metabolites was performed to investigate effect probiotics formula compared control and within first months life healthy neonates. broad metabolomics approach conceptualized describe polar semi-polar feeding type year life. Fecal metabolomes were clearly distinct between formula- breastfed...
The protozoan parasite Giardia lamblia enhances bile secretion and microbiota–mediated acid deconjugation in mice, leading to growth impairment.
BACKGROUND Growing up on traditional European, and U.S. Amish, dairy farms, in close contact with cows hay, protects children against asthma, airway administration of extracts from dust collected cowsheds those farms prevents allergic asthma mice. OBJECTIVES This study sought to begin identifying farm-derived asthma-protective agents. METHODS Our work unfolded along two unbiased, independent but complementary discovery paths. Dust protective non-protective (European Amish vs. European sheep...
Abstract Background Protein-energy malnutrition (PEM) in childhood is associated with increased susceptibility to infections, endocrine disorders, metabolic syndrome and organ dysfunction later life1-4. However, the mechanisms behind these long-term effects are poorly understood. While gut microbiome plays an important role aetiology of PEM, little known about epithelial changes caused by microbiome5-7. The aim this project was analyse PEM on intestinal epithelium presence absence...
Abstract Aging is accompanied by considerable changes in the gut microbiome, yet molecular mechanisms driving aging and role of microbiome remain unclear. Here we combined metagenomics, transcriptomics metabolomics from mice with metabolic modelling to characterize host–microbiome interactions during aging. Reconstructing integrated models host 181 mouse microorganisms, show a complex dependency metabolism on known previously undescribed microbial interactions. We observed pronounced...
Gut microbiota-derived metabolites play a pivotal role in the maintenance of intestinal immune homeostasis. Here, we demonstrate that human commensal
Exposure to high concentrations of Manganese (Mn) is known potentially induce an accumulation in the brain, leading a Parkinson related disease, called manganism. Versatile mechanisms Mn-induced brain injury are discussed, with inactivation mitochondrial defense against oxidative stress being major one. So far, studies indicate that main Mn-species entering low molecular mass (LMM) compounds such as Mn-citrate. Applying single dose MnCl2 injection rats, we observed alterations pattern within...
A metabolic disorder such as Type-2 Diabetes mellitus (T2DM) is a complex disease induced by genetic, environmental, and nutritional factors. The db/db mouse model, bearing nonfunctional leptin receptor, widely used to investigate the pathophysiology of T2DM. Fecal extracts wild-type littermates were studied unravel broad spectrum new relevant metabolites related T2DM proxies interplay gut microbiome murine metabolomes. nontargeted metabolomics approach consists an integrated analytical...
Food processing of infant formula alters chemical structures, including the formation Maillard reaction products between proteins and sugars. We detected early products, so-called Amadori in stool samples formula-fed infants. In total, four (N-deoxylactulosyllysine, N-deoxyfructosyllysine, N-deoxylactulosylleucylisoleucine, N-deoxyfructosylleucylisoleucine) were identified by a combination complementary nontargeted targeted metabolomics approaches. Chemical structures confirmed preparation...