A5041616743- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Diet and metabolism studies
- Probiotics and Fermented Foods
- Microbial Metabolic Engineering and Bioproduction
- Nutrition, Genetics, and Disease
- Coffee research and impacts
- Analytical Chemistry and Chromatography
- Spectroscopy and Chemometric Analyses
- Nutritional Studies and Diet
- Escherichia coli research studies
- Adipose Tissue and Metabolism
- Fermentation and Sensory Analysis
- Evolution and Genetic Dynamics
- Chronic Kidney Disease and Diabetes
- Infant Nutrition and Health
- Biochemical Analysis and Sensing Techniques
- Food Chemistry and Fat Analysis
- Dysphagia Assessment and Management
- Eicosanoids and Hypertension Pharmacology
- Trauma and Emergency Care Studies
- Food Quality and Safety Studies
- Chromatography in Natural Products
- Meat and Animal Product Quality
- Cerebral Palsy and Movement Disorders
Helmholtz Zentrum München
2016-2025
Helmholtz Association of German Research Centres
2024
German Center for Diabetes Research
2012-2022
Heinrich Heine University Düsseldorf
2015-2022
Deutsches Diabetes-Zentrum e.V.
2015-2022
Imperial College London
2010-2015
Center for Environmental Health
2015
University of California, Davis
2014
Medical Research Council
2010
Northwestern University
2010
Abstract Circulating trimethylamine N-Oxide (TMAO) levels predict cardiovascular disease (CVD), possibly by impacting on cholesterol metabolism and oxidative stress. Because hepatic TMAO production is regulated insulin signalling it unclear whether to what extent circulating associate with CVD risk, independently of resistance its important determinants fatty liver visceral obesity, we have now addressed this question in 220 subjects who participated the Tübingen Lifestyle Intervention...
ABSTRACT Diet can influence the composition of human microbiome, and yet relatively few dietary ingredients have been systematically investigated with respect to their impact on functional potential microbiome. Dietary resistant starch (RS) has shown health benefits, but we lack a mechanistic understanding metabolic processes that occur in gut during digestion RS. Here, collected samples crossover study diets containing large or small amounts We determined RS microbiome pathways gut, using...
High-resolution spectroscopic profiles of biofluids can define metabolic phenotypes, providing a window onto the impact diet on health to reflect gene-environment interactions. (1)H NMR profiling was used characterize effect nutritional intervention stability phenotype 7 individuals following controlled day dietary protocol. Inter-individual differences influenced proportionally more spectrum than modulation, with certain displaying greater phenotypes others. Correlation structures between...
The gut microbiota generates a huge pool of unknown metabolites, and their identification characterization is key challenge in metabolomics. However, there are still gaps on the studies chemical structures. In this investigation, an unusual class bacterial sulfonolipids (SLs) detected mouse cecum, which was originally found environmental microbes. We have performed detailed molecular level lipids by combining high-resolution mass spectrometry liquid chromatography analysis. Eighteen SLs that...
Flaxseeds contain the phytoestrogens lignans that must be activated to enterolignans by intestinal bacteria. We investigated impact of flaxseeds on fecal bacterial communities and their associations with blood metabolites.Nine healthy male adult subjects ingested 0.3 g/kg/day during 1 week. Gut bacteria as well metabolites were analyzed. Ingestion triggered a significant increase in concentration enterolignans, accompanied excretion propionate glycerol. Overall diversity composition dominant...
Specific and sensitive food biomarkers are necessary to support dietary intake assessment link nutritional habits potential impact on human health. A multistep intervention study was conducted suggest novel for coffee consumption. (1)H NMR metabolic profiling combined with multivariate data analysis resolved 2-furoylglycine (2-FG) as a putative biomarker We relatively quantified 2-FG in the urine of drinkers investigated its origin, metabolism, excretion kinetics. When searching precursors,...
Chronic kidney disease (CKD) is a recognized global health problem. While some CKD patients remain stable after initial diagnosis, others can rapidly progress towards end-stage renal (ESRD). This makes biomarkers capable of detecting progressive forms extremely valuable, especially in non-invasive biofluids such as urine. Screening for metabolite markers using non-targeted metabolomic techniques like nuclear magnetic resonance spectroscopy increasingly applied to research. A cohort (n = 227)...
The metabolic role of d-serine, a non-proteinogenic NMDA receptor co-agonist, is poorly understood. Conversely, inhibition pancreatic receptors as well loss the d-serine producing enzyme serine racemase have been shown to modulate insulin secretion. Thus, we aim study impact chronic and acute supplementation on secretion other parameters glucose homeostasis. We apply MALDI FT-ICR mass spectrometry imaging, NMR based metabolomics, 16s rRNA gene sequencing gut microbiota in combination with...
Adipose tissue expansion, as seen in obesity, is often metabolically detrimental causing insulin resistance and the metabolic syndrome. However, white adipose expansion at early ages essential to establish a functional metabolism. To understand differences between adolescent adult we studied cellular composition of stromal vascular fraction subcutaneous two eight weeks old mice using single cell RNA sequencing. We identified subset preadipocytes expressing mature adipocyte marker Asc-1 that...
In chronic kidney disease, hyperphosphatemia is a key pathological factor promoting medial vascular calcification, common complication associated with cardiovascular events and mortality. This active pathophysiological process involves osteo-/chondrogenic transdifferentiation of smooth muscle cells (VSMCs) via complex intracellular mechanisms that are still incompletely understood. Little known about the effects phosphate on bioenergetic profile VSMCs during onset this process. Therefore,...
In metabolomics, nuclear magnetic resonance (NMR) spectroscopy allows to identify and quantify compounds in biological samples. The sample preparation generally requires only few steps; however, an indispensable factor is the addition of a locking substance into biofluid sample, such as deuterium oxide (D2O). While creatinine loss pure D2O well-described, effects different concentrations on signal profile samples are unknown. this work, we investigated effect levels NMR buffer system urine...
Food processing of infant formula alters chemical structures, including the formation Maillard reaction products between proteins and sugars. We detected early products, so-called Amadori in stool samples formula-fed infants. In total, four (N-deoxylactulosyllysine, N-deoxyfructosyllysine, N-deoxylactulosylleucylisoleucine, N-deoxyfructosylleucylisoleucine) were identified by a combination complementary nontargeted targeted metabolomics approaches. Chemical structures confirmed preparation...
The process of weaning causes a major shift in intestinal microbiota and is critical period for developing appropriate immune responses young mammals.To use new systems approach to provide an overview host metabolism the system response nutritional intervention around period.Piglets (n=14) were weaned onto either egg-based or soya-based diet at 3 weeks until 7 weeks, when all piglets switched fish-based diet. Half animals on each received Bifidobacterium lactis NCC2818 supplementation from...
Abstract Restoration works in the old Clunisian Saint-Vivant monastery Burgundy revealed an unidentified wine bottle (SV1) dating between 1772 and 1860. Chemical evidence for SV1 origin nature are presented here using non-targeted Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Nuclear Magnetic analyses. The chemical diversity was compared to red wines (Pinot Noir) from Romanée Saint Vivant appellation six different vintages spanning 1915 2009. close metabolomic signature spoke...
Glucotoxic metabolites and pathways play a crucial role in diabetic complications, new treatment options which improve glucotoxicity are highly warranted. In this study, we analyzed bezafibrate (BEZ) treated, streptozotocin (STZ) injected mice, showed an improved glucose metabolism compared to untreated STZ animals. order identify key molecules participate the beneficial effects of BEZ, studied plasma, skeletal muscle, white adipose tissue (WAT) liver samples using non-targeted metabolomics...
Inflammatory bowel disease (IBD) is a persistent inflammatory condition that affects the gastrointestinal tract and presents significant challenges in its management treatment. Despite knowledge within-host bacterial evolution occurs intestine, has rarely been studied from an evolutionary perspective. In this study, we aimed to investigate of resident bacteria during intestinal inflammation whether- how disease-related genetic changes may present trade-offs with potential therapeutic...
Comprehensive non-targeted analysis of food products normally requires two complementary chromatographic runs to achieve maximum compound coverage. In this study, we present a sensitive tandem-LC method, which combines RP and HILIC separation in single run. The setup consists C18 trap column subsequently coupled analytical columns (HILIC C18) are operated parallel. First, hydrophobic compounds retained on the while rather hydrophilic directly transferred onto phase. Next, pre-fractionated...
Despite the gut's longitudinal specialization for digestion and microbiome organization, most studies focus on analysis of its end product, feces. To determine metabolic physiological functions different sections gut, we aimed to define a comprehensive list characteristic metabolites gut quantify selected pathways. We investigated composition seven from four C57Bl/6N mice with nontargeted metabolite profiling using high-resolution NMR spectroscopy, which returned overview single analytical...