A5054986782- Renal Transplantation Outcomes and Treatments
- Endoplasmic Reticulum Stress and Disease
- Dialysis and Renal Disease Management
- interferon and immune responses
- Organ Transplantation Techniques and Outcomes
- DNA Repair Mechanisms
- Helicobacter pylori-related gastroenterology studies
- Cancer Diagnosis and Treatment
- Complement system in diseases
- Oral health in cancer treatment
- Heat shock proteins research
- Genetic factors in colorectal cancer
- Genomics, phytochemicals, and oxidative stress
- Liver Disease and Transplantation
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Healthcare cost, quality, practices
- Cancer Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Cytomegalovirus and herpesvirus research
- RNA regulation and disease
- Polyomavirus and related diseases
- Digestive system and related health
- Head and Neck Cancer Studies
- Renal Diseases and Glomerulopathies
Kiel University
2019-2025
University Hospital Schleswig-Holstein
2018-2024
University of Lübeck
2018-2024
University Clinic for Nephrology and Hypertension, Diabetology and Endocrinology
2023
Excess and unresolved endoplasmic reticulum (ER) stress in intestinal epithelial cells (IECs) promotes inflammation. Activating transcription factor 6 (ATF6) is one of the signaling mediators ER stress. We studied pathways that regulate ATF6 its role for inflammation IECs.We performed an RNA interference screen, using 23,349 unique small interfering RNAs targeting 7783 genes a luciferase reporter controlled by ATF6-dependent ERSE (ER stress-response element) promoter, to identify proteins...
Background & AimsRNase H2 is a holoenzyme, composed of 3 subunits (ribonuclease A, B, and C), that cleaves RNA:DNA hybrids removes mis-incorporated ribonucleotides from genomic DNA through ribonucleotide excision repair. Ribonucleotide incorporation by eukaryotic polymerases occurs during every round genome duplication produces the most frequent type naturally occurring lesion. We investigated whether intestinal epithelial proliferation requires RNase function activity disrupted...
Background & AimsThroughout life, the intestinal epithelium undergoes constant self-renewal from stem cells. Together with genotoxic stressors and failing DNA repair, this causes susceptibility toward malignant transformation. X-box binding protein 1 (XBP1) is a stress sensor involved in unfolded response (UPR). We hypothesized that XBP1 acts as signaling hub to regulate epithelial damage responses.MethodsData The Cancer Genome Atlas were analyzed for association of colorectal cancer (CRC)...
Rigid age limits in the current allocation system for post-mortem donor kidneys Germany may have problematic effects. The new German national transplantion registry enables data analysis with respect to this question. Using anonymized from registry, we extracted and evaluated information on recipients postmortem donors of that were allocated through Eurotransplant over period 2006-2020. Data 19 664 kidney transplantations 2006 2020 analyzed. median waiting time transplantation was 5.8 years....
Abstract Background RNaseH2 plays a critical role in removing mis-bounded RNA from the DNA double strand helix1. Absence of results spontaneous double-stranded breaks and cytosolic release dsDNA2. We have previously shown that epithelial deficiency RNaseH2b (H2bΔIEC) leads to p53-dependent stem cell suppression additional p53 intestinal carcinogenesis3. Due central cGAS/STING sensing dsDNA, we aim investigate STING mouse model chronic damage tumor prevention this study. Methods was assessed...
Abstract Background Stimulator of interferon genes (STING) regulates intestinal homeostasis and inflammation by detecting cytoplasmic double-stranded DNA, driving type-1 (IFN) responses.1 The autophagy-related gene ATG16L1, a major risk factor for inflammatory bowel disease (IBD), links to STING overactivation in epithelial cells (IEC), causing via IFN1, NF-κB, TNFα signaling.2 Excessive activity is associated with autoinflammatory disorders1, whereas full-body Sting knockout (Tmem173gt/gt)...
Background. Kidney graft rejections are classified based on the Banff classification. The RejectClass algorithm, initially derived from a cohort comprising mostly protocol biopsies, identifies data-driven phenotypes of acute rejection and chronic pathology using lesion scores. It also provides composite scores for inflammation activity chronicity. This study independently evaluates performance in consisting entirely indication biopsies. Methods. We retrospectively applied to 441 patients...
Early-on post kidney transplantation, there is a high risk of graft rejection and opportunistic viral infections. A low tacrolimus concentration/dose (C/D) ratio as surrogate marker fast metabolism has been established for stratification 3 months post-transplantation (M3). However, many adverse events occurring earlier might be missed, at 1 month (M1) not investigated. We retrospectively analyzed case data from 589 patients who had undergone transplantation between 2011 2021 three German...
Abstract Background Peritoneal dialysis (PD) remains underutilised in Germany, prompting the initiation of Sustainable Intensification Dialysis Schleswig–Holstein (SKIP-SH) project. The SKIP-SH cohort study aims to demonstrate presumed benefits PD, including enhanced quality life and reduced healthcare personnel requirements, generate data strengthen use PD. Methods prospective recruits patients with advanced chronic kidney disease (CKD) their caregivers. Comprehensive data, demographic...
IntroductionThe time from dialysis onset to enrollment on the kidney waiting list (listing time) is a crucial step path receiving allograft, but this process has received very little research attention in Eurotransplant area.MethodsWe retrospectively analyzed data German transplantation registry including patients who were for first transplant Germany between 2006 and 2016. Listing was evaluated using mixed linear model. The outcomes assessed competing risk analyses.ResultsWe total of 43,955...
Autophagie ist wichtig für den Abbau von cytoplasmatischen Makromolekülen. Für die Funktionalität dieses Pathways das CED-Risikogen ATG16L1 entscheidend. Auswirkungen Mutationen Gens auf DNA-Schäden sind aber weitestgehend unbekannt. Wir konnten in der Vergangenheit zeigen, dass Defizienz des Enzyms RNase H2b zu gestörter Nukleotid-Exzisions-Reperatur und gehäuften Doppelstrang-Brüchen führt mit p53- abhängiger verminderter Proliferation Stammzellen. Fehlten p53 RNAseH2b führte dies...
Vorstudien zeigen, dass RNase H2 die genomische Integrität von intestinalen Stammzellen (ISC) mittels Ribonukleotidexzisionsreparatur bewahrt. Bei H2-Verlust verursachen DNA-Schäden in Epithelzellen (IEC) eine p53-gesteuerte, vor intestinaler Karzinogenese schützende ISC-Suppression (Aden, Bartsch et al. 2019). XBP1 kodiert für einen Transkriptionsfaktor, dessen Funktion sich als essenziell Resolution ER-Stress erwiesen hat (Kaser, Lee 2008).
Introduction The unfolded protein response (UPR) and macro-autophagy are key cellular homeostatic pathways mutations of genes involved in these associated with intestinal bowel disease (IBD)1,2. We have previously shown that deficiency the enzyme RNaseH2b3,4 leads to impaired ribonucleotide excision repair induced spontaneous DNA damage subsequent suppression stem cell proliferation.