A5043212905- Bacterial biofilms and quorum sensing
- Cystic Fibrosis Research Advances
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Inhalation and Respiratory Drug Delivery
- Human auditory perception and evaluation
- Advanced Chemical Sensor Technologies
- Health and Medical Studies
- Health Promotion and Cardiovascular Prevention
- 3D Printing in Biomedical Research
- Helicobacter pylori-related gastroenterology studies
- Probiotics and Fermented Foods
- interferon and immune responses
- Infections and bacterial resistance
- Clinical practice guidelines implementation
- Dupuytren's Contracture and Treatments
- Mycobacterium research and diagnosis
- Cardiovascular Effects of Exercise
- Autoimmune and Inflammatory Disorders Research
- Gut microbiota and health
- Cancer Cells and Metastasis
- Antimicrobial Peptides and Activities
- Genetics and Physical Performance
Kiel University
2025
Ghent University
2019-2024
Pseudomonas aeruginosa is a prominent cystic fibrosis (CF) pathogen that uses quorum sensing (QS) to regulate virulence. In laboratory strains, the key QS regulator LasR. Many isolates from patients with chronic CF infections appear use an alternate circuitry in which another transcriptional regulator, RhlR, mediates QS. We show LasR-null clinical isolate engages through RhlR and remains capable of inducing cell death vivo- like lung epithelium model. Our findings support notion can engage...
Patients with chronic lung disease suffer from persistent inflammation and are typically colonized by pro-inflammatory pathogenic bacteria. Besides these pathogens, a wide variety of commensal species is present in the lower airways but their role unclear. Here, we show that microbiota contains several able to inhibit activation NF-κB pathway production interleukin 8 (IL-8), triggered lipopolysaccharide (LPS) or H 2 O , physiologically relevant three-dimensional (3D) epithelial cell model....
Antimicrobial peptides (AMPs) are promising templates for the development of novel antibiofilm drugs. Despite large number studies on screening and optimization AMPs, only a few these evaluated activity in physiologically relevant model systems. Potent vitro AMPs often does not translate into vivo effectiveness due to interference host microenvironment with peptide stability/availability. Hence, mimicking complex environment found biofilm-associated infections is essential predict clinical...
Models to study host-pathogen interactions in vitro are an important tool for investigating the infectious disease process and evaluating efficacy of antimicrobial compounds. In these models, viability mammalian cells is often determined using lactate dehydrogenase (LDH) cytotoxicity assay. present we evaluated whether bacteria could interfere with LDH As a model interactions, co-cultured lung epithelial eight encountered lower respiratory tract. We show that activity affected by Pseudomonas...
The presence of Pseudomonas aeruginosa biofilms in cystic fibrosis (CF) patients suffering from chronic lung infections contributes to the failure antimicrobial therapy. Conventionally, minimal inhibitory concentration (MIC) is determined assess susceptibility a pathogen, however this parameter fails predict success treating biofilm-associated infections. In present study we developed high throughput method determine required prevent P. biofilm formation, using synthetic sputum medium...
Abstract Background Stimulator of interferon genes (STING) regulates intestinal homeostasis and inflammation by detecting cytoplasmic double-stranded DNA, driving type-1 (IFN) responses.1 The autophagy-related gene ATG16L1, a major risk factor for inflammatory bowel disease (IBD), links to STING overactivation in epithelial cells (IEC), causing via IFN1, NF-κB, TNFα signaling.2 Excessive activity is associated with autoinflammatory disorders1, whereas full-body Sting knockout (Tmem173gt/gt)...
In vitro models of differentiated respiratory epithelium that allow high-throughput screening are an important tool to explore new therapeutics for chronic diseases. the present study, we developed in vivo-like three-dimensional (3-D) bronchial epithelial cell lines commonly used study lung disease (16HBE14o-, CFBE41o- and 6.2 WT-CFTR). To this end, cells were cultured on porous microcarrier beads rotating wall vessel (RWV) bioreactor, optimized suspension culture method allows higher...
People with cystic fibrosis (pwCF) often suffer from chronic lung infections Pseudomonas aeruginosa. While antibiotics are still commonly used to treat P. aeruginosa infections, there is a high discordance between in vitro and vivo antibiotic efficacy, which contributes suboptimal therapy. In the present study, we found that isolates same sputum sample had highly diverse resistance profiles [based on minimal inhibitory concentration (MIC)], may explain reported discrepancy efficacy. Through...
It is increasingly recognized that interspecies interactions may modulate the pathogenicity of
ABSTRACT The opportunistic pathogen Pseudomonas aeruginosa is a leading cause of airway infection in cystic fibrosis (CF) patients. P. employs several hierarchically arranged and interconnected quorum sensing (QS) regulatory circuits to produce battery virulence factors such as elastase, phenazines, rhamnolipids. QS transcription factor LasR sits atop this hierarchy, activates the dozens genes, including that encoding regulator RhlR. Paradoxically, inactivating lasR mutations are frequently...