A5103019975- Breast Cancer Treatment Studies
- DNA Repair Mechanisms
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Radiomics and Machine Learning in Medical Imaging
- MRI in cancer diagnosis
- PARP inhibition in cancer therapy
- BRCA gene mutations in cancer
- Cancer Genomics and Diagnostics
- Glycosylation and Glycoproteins Research
- Lung Cancer Treatments and Mutations
- Immune cells in cancer
- Immune Cell Function and Interaction
- Advanced Neuroimaging Techniques and Applications
- Cervical Cancer and HPV Research
- Cancer, Stress, Anesthesia, and Immune Response
- Advanced Breast Cancer Therapies
- Photoacoustic and Ultrasonic Imaging
- Endometrial and Cervical Cancer Treatments
- Optical Imaging and Spectroscopy Techniques
- Phagocytosis and Immune Regulation
- Thermography and Photoacoustic Techniques
- RNA Research and Splicing
- Advances in Oncology and Radiotherapy
- Breast Lesions and Carcinomas
King's College London
2015-2021
Kings Health Partners
2017-2021
Ontario Institute for Cancer Research
2021
Guy's Hospital
2016-2020
Breast Cancer Now
2016-2017
King's College School
2015
King's College - North Carolina
2015
Abstract Introduction: Subgroups within sporadic triple negative breast cancers (TNBCs) appear to share impaired DNA damage response mechanisms with BRCA1/2 mutation-associated cancers. This has been hypothesised confer particular sensitivity DNA-damaging platinum chemotherapy. The TNT trial, a randomized phase III trial in women metastatic or recurrent locally advanced TNBC cancer, aimed test this hypothesis and examine treatment effect biological subgroups. Patients & Methods: Eligible...
An innate-like γδ T cell compartment in healthy human breast is described and shown to correlate with remission triple-negative cancer.
Abstract Cancer cells tend to metastasize first tumor-draining lymph nodes, but the mechanisms mediating cancer cell invasion into lymphatic vasculature remain little understood. Here, we show that in human breast tumor microenvironment (TME), presence of increased numbers RORγt+ group 3 innate lymphoid (ILC3) correlates with an likelihood node metastasis. In a preclinical mouse model cancer, CCL21-mediated recruitment ILC3 tumors stimulated production CXCL13 by TME stromal cells, which turn...
In early-stage breast cancer, the primary treatment option for most women is breast-conserving surgery (BCS). There a clear need more accurate techniques to assess resection margins intraoperatively, because on average 20% of patients require further achieve margins. Cerenkov luminescence imaging (CLI) combines optical and molecular by detecting light emitted <sup>18</sup>F-FDG. Its high-resolution small size equipment make CLI promising technology intraoperative margin assessment. A...
Since nearly 20% of breast-conserving surgeries (BCS) require re-operation, there is a clear need for developing new techniques to more accurately assess tumor resection margins intraoperatively. This study evaluates the diagnostic accuracy handheld terahertz pulsed imaging (TPI) system discriminate benign from malignant breast tissue ex vivo. Forty six freshly excised cancer samples were scanned with TPI probe system, and histology was obtained comparison. The image pixels on classified...
Abstract The tumour microenvironment plays a crucial role in the growth and progression of cancer, presence tumour-associated macrophages (TAMs) is associated with poor prognosis. Recent studies have demonstrated that TAMs display transcriptomic, phenotypic, functional geographical diversity. Here we show sialylated glycoform mucin MUC1, MUC1-ST, through engagement Siglec-9 can specifically independently induce differentiation monocytes into unique phenotype to best our knowledge has not...
Highlights•β4 integrin-expressing macrophages release TGF-β1 near breast cancer lymphovasculature•TGF-β1 drives β4 integrin clustering on macrophages, enhancing macrophage adhesion•TGF-β1 signals through RhoA to drive lymphatic endothelial cell contraction•Lymphatic remodeling signaling cascade facilitates metastasisSummaryLymphatic vasculature is crucial for metastasis in triple-negative (TNBC); however, cellular and molecular drivers controlling lymphovascular are poorly understood. We...
The diffusion signal in breast tissue has primarily been modelled using apparent coefficient (ADC), intravoxel incoherent motion (IVIM) and tensor (DT) models, which may be too simplistic to describe the underlying microstructure. Formalin‐fixed cancer samples were scanned a wide range of gradient strengths, durations, separations orientations. A variety one‐ two‐compartment models tested determine best described data. Models with restricted components anisotropy selected most cancerous...
Abstract The prognostic importance of lymph node (LN) status and tumour‐infiltrating lymphocytes (TILs), is well established, particularly TILs in triple negative breast cancers (TNBCs). So far, few studies have interrogated changes involved uninvolved LNs evaluated if their morphological patterns add valuable information for the prediction disease progression cancer. In a cohort 309 patients enriched TNBCs (170/309), we histologically characterised immune stromal features primary tumours...
•Patients with intermediate levels of allelic imbalanced CNAs show a better response rate to carboplatin in TNT.•The lack amplifications on 1q, 8q and 10p is associated superior relation between chromosomal instability primary tumours advanced settings non-linear. BackgroundIn the TNT trial triple negative breast cancer (NCT00532727), germline BRCA1/2 mutations were present 28% responders. We assessed quantitative measures structural (CIN) identify wider patient subgroup within preferential...
Abstract In breast cancer (BC), detecting low volumes of axillary lymph node (ALN) metastasis pre-operatively is difficult and novel biomarkers are needed. We recently showed that patient-derived ALNs can be sustained ex-vivo using normothermic perfusion. now compare reactive (tumour-free; n = 5) macrometastatic (containing tumour deposits >2 mm; 4) by combining whole section multiplex immunofluorescence with TMT-labelled LC-MS/MS the circulating perfusate. Macrometastases contained...
Understanding how breast cancer (BC) grows in axillary lymph nodes (ALNs), and refining therapies might halt that process, is clinically important. However, modelling the complex ALN microenvironment difficult, no human models exist at present. We harvested ALNs from ten BC patients, perfused them 37 °C ex vivo for up to 24 h. Controlled autologous testing showed remain viable after h of perfusion: haematoxylin eosin-stained histological appearance proliferation (by Ki67...
1019 Background: The majority of triple negative breast cancers are basal-like subtype (BLBC). There is uncertainty about concordance intrinsic subtypes and ROR scores by PAM50 between matched primary metastasis samples. We sought to examine agreement groups using tissue in the TNT trial. Methods: a phase III, multicentre, randomized trial carboplatin vs docetaxel women with ER- PgR- HER2- metastatic/recurrent locally advanced cancer. Using Prosigna test on Nanostring nCounter, subtyping was...
Abstract The tumour microenvironment plays a crucial role in the growth and progression of cancer presence tumour-associated macrophages (TAMs) is associated with poor prognosis. Recent studies show that TAMs transcriptomic, phenotypic, functional geographical diversity. Here we sialylated glycoform mucin MUC1, MUC1-ST, through engagement Siglec-9 can specifically independently induce differentiation monocytes into unique phenotype. These recruit maintain neutrophils, inhibit function T...