A5081451328- Protein Degradation and Inhibitors
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Computational Drug Discovery Methods
- Bioinformatics and Genomic Networks
- Melanoma and MAPK Pathways
- Epigenetics and DNA Methylation
- Advanced Proteomics Techniques and Applications
- vaccines and immunoinformatics approaches
- Synthesis and biological activity
- Plant nutrient uptake and metabolism
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Plant-Microbe Interactions and Immunity
- Gene expression and cancer classification
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
- Salmonella and Campylobacter epidemiology
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- interferon and immune responses
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
Institute of Cancer Research
2017-2025
Heze University
2025
Liaocheng University
2025
Union Hospital
2018-2025
Huazhong University of Science and Technology
2018-2025
Suzhou University of Science and Technology
2025
Shanghai First Maternity and Infant Hospital
2023-2024
Huazhong Agricultural University
2008-2024
First Hospital of Shanxi Medical University
2024
Shanxi Medical University
2024
Characterization of the composition postsynaptic proteome (PSP) provides a framework for understanding overall organization and function synapse in normal pathological conditions. We have identified 698 proteins from terminal mouse CNS synapses using series purification strategies analysis by liquid chromatography tandem mass spectrometry large-scale immunoblotting. Some 620 were found purified densities (PSDs), nine AMPA-receptor immuno-purifications, 100 isolates an antibody against NMDA...
Sound scoring methods for sequence database search algorithms such as Mascot and Sequest are essential sensitive accurate peptide protein identifications from proteomic tandem mass spectrometry data. In this paper, we present a software package that interfaces with Percolator, well performing machine learning method rescoring results, demonstrate it to be amenable both low high accuracy data, outperforming all available schemes providing reliable significance measures. Percolator can readily...
The transcription factor Oct4 is key in embryonic stem cell identity and reprogramming. Insight into its partners should illuminate how the pluripotent state established regulated. Here, we identify a considerably expanded set of Oct4-binding proteins mouse cells. We find that associates with varied including regulators gene expression modulators function. Half are transcriptionally regulated by itself or other factors, whereas one-third display significant change upon differentiation....
WRKY proteins form a large family of plant transcription factors implicated in the modulation numerous biological processes, such as growth, development and responses to various environmental stresses. However, roles majority members, especially non-model plants, remain poorly understood. We identified CaWRKY40 from pepper. Transient expression onion epidermal cells showed that can be targeted nuclei activates W-box-containing reporter gene. transcripts are induced pepper by Ralstonia...
A family of apicomplexa-specific proteins containing AP2 DNA-binding domains (ApiAP2s) was identified in malaria parasites. This includes sequence-specific transcription factors that are key regulators development. However, functions for the majority ApiAP2 genes remain unknown. Here, a systematic knockout screen Plasmodium berghei ten were essential mosquito transmission: four critical formation infectious ookinetes, and three required sporogony. We describe non-essential AP2-O AP2-SP blood...
Abstract Poly (ADP-ribose) polymerase (PARP) inhibitors elicit antitumour activity in homologous recombination-defective cancers by trapping PARP1 a chromatin-bound state. How cells process trapped remains unclear. Using wild-type and trapping-deficient mutant combined with rapid immunoprecipitation mass spectrometry of endogenous proteins Apex2 proximity labelling, we delineated spectrometry-based interactomes non-trapped PARP1. These analyses identified an interaction between the...
In the nervous system, protein phosphorylation is an essential feature of synaptic function. Although known to be important for many processes and in disease, little about global proteins. Heterogeneity low abundance make analysis difficult, particularly mammalian tissue samples. Using a new approach, combining both peptide immobilized metal affinity chromatography mass spectrometry data acquisition strategies, we have produced first large scale map mouse synapse phosphoproteome. We report...
High-density, strand-specific cDNA sequencing (ssRNA–seq) was used to analyze the transcriptome of Salmonella enterica serovar Typhi (S. Typhi). By mapping sequence data entire S. genome, we analyzed in a manner and further defined transcribed regions encoded within prophages, pseudogenes, previously un-annotated, 3′- or 5′-untranslated (UTR). An additional 40 novel candidate non-coding RNAs were identified beyond those annotated. Proteomic analysis combined with confirm refine annotation...
ABSTRACT Clostridium difficile , a major cause of antibiotic-associated diarrhea, produces highly resistant spores that contaminate hospital environments and facilitate efficient disease transmission. We purified C. using novel method show they exhibit significant resistance to harsh physical or chemical treatments are also infectious, with <7 environmental per cm 2 reproducibly establishing persistent infection in exposed mice. Mass spectrometric analysis identified ∼336 spore-associated...
We analyzed the mouse forebrain cytosolic phosphoproteome using sequential (protein and peptide) IMAC purifications, enzymatic dephosphorylation, targeted tandem mass spectrometry analysis strategies. In total, complementary phosphoenrichment LC-MS/MS strategies, 512 phosphorylation sites on 540 non-redundant phosphopeptides from 162 phosphoproteins were characterized. Analysis of protein domains amino acid sequence composition this data set revealed that it is significantly enriched in...
WRKY proteins are encoded by a large gene family and linked to many biological processes across range of plant species. The functions underlying mechanisms have been investigated primarily in model plants such as Arabidopsis rice. roles these transcription factors non-model plants, including pepper other Solanaceae, poorly understood. Here, we characterize the expression function subgroup IIe protein from (Capsicum annuum), denoted CaWRKY27. localized nuclei activated reporter GUS construct...
Clostridium difficile is an anaerobic, Gram-positive bacterium that can reside as a commensal within the intestinal microbiota of healthy individuals or cause life-threatening antibiotic-associated diarrhea in immunocompromised hosts. C. also form highly resistant spores are excreted facilitating host-to-host transmission. The spo0A gene encodes conserved transcriptional regulator sporulation required for relapsing disease and transmission mice. Here we describe genome-wide approach using...
Recent advances in proteomic mass spectrometry (MS) offer the chance to marry high-throughput peptide sequencing transcript models, allowing validation, refinement, and identification of new protein-coding loci. We present a novel pipeline that integrates highly sensitive statistically robust spectrum matching with genome-wide predictions perform large-scale gene validation discovery mouse genome for first time. In searching an excess 10 million spectra, we have been able validate 32%, 17%,...
A GGGGCC hexanucleotide repeat expansion within the C9orf72 gene is most common genetic cause of both amyotrophic lateral sclerosis and frontotemporal dementia. Sense antisense repeat-containing transcripts undergo repeat-associated non-AUG-initiated translation to produce five dipeptide proteins (DPRs). The polyGR polyPR DPRs are extremely toxic when expressed in Drosophila neurons. To determine mechanism that mediates this toxicity, we purified from brain used mass spectrometry identify...
Abstract The midbody is an organelle assembled at the intercellular bridge between two daughter cells end of mitosis. It controls final separation and has been involved in cell fate, polarity, tissue organization, cilium lumen formation. Here, we report characterization intricate protein-protein interaction network (interactome), which identifies many previously unknown interactions provides extremely valuable resource for dissecting multiple roles midbody. Initial analysis this interactome...
Significance Nitrogen availability has a large impact on plant biomass and crop production. Both depletion excessive application of nitrogen affect yields quality disturb important ecosystems. Thus, understanding the mechanisms by which regulates developmental transitions affecting reproduction, such as flowering, is to sustainably improve yields. Our study identified FBH4 key transcription factor mediating nitrogen-responsive flowering in Arabidopsis . levels control phosphorylation state...
Necroptosis is a lytic, inflammatory form of cell death that not only contributes to pathogen clearance but can also lead disease pathogenesis. triggered by RIPK3-mediated phosphorylation MLKL, which thought initiate MLKL oligomerisation, membrane translocation and rupture, although the precise mechanism incompletely understood. Here, we show K63-linked ubiquitin chains are attached during necroptosis ubiquitylation at K219 significantly cytotoxic potential phosphorylated MLKL. The K219R...
Abstract Genomic instability arising from defective responses to DNA damage 1 or mitotic chromosomal imbalances 2 can lead the sequestration of in aberrant extranuclear structures called micronuclei (MN). Although MN are a hallmark ageing and diseases associated with genomic instability, catalogue genetic players that regulate generation remains be determined. Here we analyse 997 mouse mutant lines, revealing 145 genes whose loss significantly increases ( n = 71) decreases 74) formation,...
Summary WRKY transcription factors are encoded by large gene families across the plant kingdom. So far, their biological and molecular functions in nonmodel plants, including pepper ( C apsicum annuum ) other Solanaceae, remain poorly understood. Here, we report on functional characterization of a new group I protein from pepper, termed 58. Our data indicate that 58 can be localized to nucleus activate reporter β‐glucuronidase GUS driven 35S core promoter with two copies W ‐box its proximal...
Carbon (C) and nitrogen (N) are essential elements for metabolism, their availability, called the C/N balance, must be tightly coordinated optimal growth in plants. Previously, we have identified ubiquitin ligase CNI1/ATL31 as a novel regulator by screening plants grown on stress medium containing excess sugar limited N. To elucidate further effect of balance plant to determine physiological function ATL31, performed response analysis using an atmospheric CO2 manipulation system. Under...