A5101805259- Analytical Methods in Pharmaceuticals
- Analytical Chemistry and Chromatography
- Antibiotics Pharmacokinetics and Efficacy
- Computational Drug Discovery Methods
- Inflammatory mediators and NSAID effects
- Pharmaceutical and Antibiotic Environmental Impacts
- Phenothiazines and Benzothiazines Synthesis and Activities
- Endoplasmic Reticulum Stress and Disease
- Metabolomics and Mass Spectrometry Studies
- Synthesis and Characterization of Heterocyclic Compounds
- Synthesis and Biological Evaluation
- Drug Solubulity and Delivery Systems
- Drug Transport and Resistance Mechanisms
- Chemical Synthesis and Analysis
- Antibiotic Resistance in Bacteria
- bioluminescence and chemiluminescence research
- Analytical Chemistry and Sensors
- Receptor Mechanisms and Signaling
- Vitamin C and Antioxidants Research
- Phytochemicals and Antioxidant Activities
- Biochemical and Molecular Research
- Fungal Biology and Applications
- Vitamin D Research Studies
- Cholinesterase and Neurodegenerative Diseases
- Synthesis of β-Lactam Compounds
Jagiellonian University
2016-2025
Wellcome Sanger Institute
2021-2024
Instytut Hematologii i Transfuzjologi
2015
Silesian University of Technology
2014
Wroclaw University of Environmental and Life Sciences
2013
University of Alberta
2000-2011
Canadian Institutes of Health Research
2003
Abstract Genomic instability arising from defective responses to DNA damage 1 or mitotic chromosomal imbalances 2 can lead the sequestration of in aberrant extranuclear structures called micronuclei (MN). Although MN are a hallmark ageing and diseases associated with genomic instability, catalogue genetic players that regulate generation remains be determined. Here we analyse 997 mouse mutant lines, revealing 145 genes whose loss significantly increases ( n = 71) decreases 74) formation,...
Alzheimer's disease (AD) has a complex and not-fully-understood etiology. Recently, the serotonin receptor 5-HT6 emerged as promising target for AD treatment; thus, here new series of 5-HT6R ligands with 1,3,5-triazine core selenoether linkers was explored. Among them, 2-naphthyl derivatives exhibited strong affinity selectivity over 5-HT1AR (13–15), 5-HT7R (14 15), 5-HT2AR (13). Compound 15 displayed high other central nervous system receptors low risk cardio-, hepato-, nephrotoxicity no...
Among serotonin receptors, the 5-HT6 subtype is an important protein target and its ligands may play a key role in innovative treatment of cognitive disorders. This study aimed to extend body preclinical research on two naphthyl-derived methylpiperazine-1,3,5-triazine analogues with thioether (WA-22) or Se-ether (PPK-32) linkers, newly described compounds having high affinity selectivity for receptors drug-like parameters vitro. Thus, crystallography-supported deeper insight into their...
Calreticulin is a Ca2+ -binding chaperone that resides in the lumen of endoplasmic reticulum and involved regulation intracellular homeostasis folding newly synthesized glycoproteins. In this study, we have used site-specific mutagenesis to map amino acid residues are critical calreticulin function. We focused on two cysteine (Cys(88) Cys(120)), which form disulfide bridge N-terminal domain calreticulin, tryptophan residue located carbohydrate binding site (Trp(302)), certain at tip...
The formation of cefuroxime axetil+cyclodextrin (CA+CD) complexes increases the aqueous solubility CA, improves its physico-chemical properties, and facilitates a biomembrane-mediated drug delivery process. In CD-based tablet formulations, it is crucial to investigate molecular details in final pharmaceutical preparation. this study, Raman spectroscopy mapping were applied for detection identification chemical groups involved α-, β-, γ-, 2-hydroxypropyl-β-CD (2-HP- β-CD)+CA complexation...
Lipophilicity study of selected NSAIDs, the group bioactive compounds usually used in humans and animals medicine, with use experimental calculation methods was evaluated. LogP values are proposed compared as descriptors lipophilicity eleven (from oxicams coxibs). Obtained data were designated by thin-layer chromatography (TLC) various chromatographic conditions, stationary phases different properties. The mobile phase systems prepared mixing respective amounts water organic modifier,...
In recent years, ultra-efficient liquid chromatography (UPLC) has gained particular popularity due to the possibility of faster separation small molecules. This technique, used separate ingredients present in multi-component mixtures, found application many fields, such as chemistry, pharmacy, food, and biochemistry. It is an important tool both research production. UPLC created new possibilities for analytical without reducing quality obtained results. technique therefore a milestone...
This study focuses on the design, synthesis, biological evaluation, and computer-aided structure-activity relationship (SAR) analysis for a novel group of aromatic triazine-methylpiperazines, with an hydantoin spacer between 1,3,5-traizine fragment. New compounds were synthesized their affinities serotonin 5-HT₆, 5-HT1A, 5-HT2A, 5-HT₇, dopamine D₂ receptors evaluated. The induced-fit docking (IFD) procedure was performed to explore 5-HT₆ receptor conformation space employing two lead...
The lipophilicity (R(M0)) and specific hydrophobic surface area for the representatives of four generation cephalosporins have been determined by reversed-phase thin-layer chromatography, effect different mobile-phase modifiers (such as methanol, acetonitrile, acetone, 1,4-dioxane 2-propanol) on retention has studied. compounds studied showed typical behavior; their R(M) values decreased linearly with increasing concentration organic modifier in eluent. linear correlations between volume...
Pollution of the environment with inorganic and organic substances is one main problems in world. For this reason, it necessary to conduct researches for effective methods biodegradation xenobiotics, including drugs whose unmetabolized forms are introduced into environment, especially water. One possible solution problem may be use white rot fungi, such as Lentinula edodes. This an edible species used medicine because its beneficial anti-cancer, hypocholesterolemic, hypotensive, hypoglycemic...
β-carotene as a dietary supplement is matter of interest to anyone who seeks improve their skin condition or sight. Due its antioxidative properties, carotenoids are involved in the prevention severe conditions, such cancer, heart disease, macular degeneration, cataract, which makes them an object scientific study. Thanks color, ß-carotene used food colorant, improving appearance foodstuff. A presented method was established for identification and quantification by thin-layer chromatography...
Vitamins are a unit of organic chemical substances that essential for the adequate working human body. Water-soluble B vitamins involved in regulation many metabolic and regulatory processes. Due to inability synthesize endogenously, they must be supplied body with edibles or form supplementation as drugs dietary supplements. Maintaining correct level is extremely important treatment various diseases. In presented work, qualitative quantitative procedure assay B1, B2, B5, B6 B12...
Abstract Lecithin is a mixture of phospholipids which naturally occurs in plants and animals cells. This component drugs as well dietary supplements, are used to improve memory concentration, likewise decrease cholesterol level blood, risk cardiovascular diseases lot other cases. In this paper the quantitative qualitative analysis phosphatidylcholine, main lecithin, by thin-layer chromatography with densitometric detection, was described. First, method validated according International...
Nowadays, the most important aspect related to use of dietary supplements seems be their quality. There are many reports indicating insufficient quality primarily a much lower content ingredients or even absence. Currently, there is an increasing interest in supplementing diet with various kinds supplements, including those containing combinations vitamins and minerals, among which preparations vitamin D very popular. This probably due reduced production this vitamin, depending on amount...
A rapid, accurate and sensitive thin-layer chromatography (TLC) method with densitometric detection has been developed validated for the determination of cefepime in pharmaceuticals. Chromatographic separation was achieved on a silica gel TLC F254 plates mobile phase consisting ethanol-2-propanol-glacial acetic acid 99.5%-water (4:4:1:3, v/v). Densitometric carried out at wavelength 266 nm reflectance/absorbance mode. The validation found to be satisfactory high accuracy (from 99.24 101.37%)...
Metronidazole is a drug widely used in the prevention and treatment of bacterial infections. Due to its possibility formation stable metal complexes, it was decided broaden activity spectrum by introducing silver(I) coordination compounds i.e., [Ag(MTZ)2NO3] [(Ag(MTZ)2)2]SO4, which have significant antibacterial properties. The paper presents description new qualitative quantitative analysis metronidazole bulk possible pharmaceutical preparations thin-layer chromatography with densitometric...