A5050237089- Microtubule and mitosis dynamics
- Genetic and Kidney Cyst Diseases
- Protist diversity and phylogeny
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Reproductive Biology and Fertility
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Cellular Mechanics and Interactions
- Nuclear Structure and Function
- Developmental Biology and Gene Regulation
- Genomics and Chromatin Dynamics
- Insect and Arachnid Ecology and Behavior
- Neurobiology and Insect Physiology Research
- RNA Research and Splicing
- Cellular transport and secretion
- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- Retinal Development and Disorders
- Subterranean biodiversity and taxonomy
- Insect and Pesticide Research
- Invertebrate Immune Response Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Epigenetics and DNA Methylation
- Photoreceptor and optogenetics research
University of Siena
2016-2025
University of Cologne
2021
University of Cambridge
2007
Cancer Research UK
2007
Weizmann Institute of Science
2007
University of California, Irvine
1996
The Discs large (Dlg) protein of Drosophila is the prototypic member a growing family proteins termed membrane-associated guanylate kinase homologs (MAGUKs). MAGUKs are composed series peptide domains that include one or three DHR/PDZs, an SH3, and region homologous to (GUK). We have previously shown product this gene, Dlg protein, localized at septate junctions between epithelial cells, mutations in gene cause neoplastic overgrowth imaginal discs. dlg locus therefore defined as tumor...
Abstract A mutation in the centrosomal‐P4.1‐associated protein ( CPAP ) causes Seckel syndrome with microcephaly, which is suggested to arise from a decline neural progenitor cells NPC s) during development. However, mechanisms of s maintenance remain unclear. Here, we report an unexpected role for cilium and identify as negative regulator ciliary length independent its centrosome biogenesis. At onset disassembly, provides scaffold disassembly complex CDC ), includes Nde1, Aurora A, OFD 1,...
Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated tumor development, is an upstream regulator of biogenesis necessary for centriole formation. We found that overexpression SAK/PLK4 could induce amplification centrioles Drosophila embryos and their de novo formation unfertilized eggs. Both processes required the activity DSAS-6 DSAS-4, two molecules Thus, template-free self-assembly...
A number of lines evidence point to a predominance cytokinesis defects in spermatogenesis hypomorphic alleles the Drosophila polo gene. In pre-meiotic mitoses, result cysts primary spermatocytes with reduced numbers cells that can contain multiple centrosomes. These are connected by correspondingly ring canals, structures formed stabilization cleavage furrow. The earliest during meiotic divisions failure form correct mid-zone and mid-body at telophase. This is accompanied correctly localize...
Cytokinesis partitions cytoplasmic and genomic materials at the end of cell division. Failure in this process causes polyploidy, which turn can generate chromosomal instability, a hallmark many cancers. Successful cytokinesis requires cooperative interaction between contractile ring central spindle components, but how cooperation is established poorly understood. Here we show that Sticky (Sti), Drosophila ortholog component Citron kinase (CIT-K), interacts directly with two kinesins, Nebbish...
Abstract The midbody is an organelle assembled at the intercellular bridge between two daughter cells end of mitosis. It controls final separation and has been involved in cell fate, polarity, tissue organization, cilium lumen formation. Here, we report characterization intricate protein-protein interaction network (interactome), which identifies many previously unknown interactions provides extremely valuable resource for dissecting multiple roles midbody. Initial analysis this interactome...
The behavior of parental chromosomes during the first mitosis Drosophila simulans zygotes obtained from unidirectional incompatible crosses is described and it demonstrated that condensation chromatin complements was asynchronous. timing paternal appeared to be delayed in these embryos, so condensed maternal entangled prophase-like fibers congressed equatorial plane metaphase spindle. At anaphase migrated opposite poles spindle, whereas lagged midzone This resulted dramatic errors...
Abstract Centrioles and cilia are microtubule-based structures, whose precise formation requires controlled cytoplasmic tubulin incorporation. How is recognized for centriolar/ciliary-microtubule construction remains poorly understood. Centrosomal-P4.1-associated-protein (CPAP) binds via its PN2-3 domain. Here, we show that a C-terminal loop-helix in targets β-tubulin at the microtubule outer surface, while an N-terminal helical motif caps microtubule’s α-β surface of β-tubulin. Through...
Glioblastoma multiforme (GBM) possesses glioma stem cells (GSCs) that promote self-renewal, tumor propagation, and relapse. Understanding the mechanisms of GSCs self-renewal can offer targeted therapeutic interventions. However, insufficient knowledge GSCs' fundamental biology is a significant bottleneck hindering these efforts. Here, we show patient-derived recruit elevated levels proteins ensure temporal cilium disassembly, leading to suppressed ciliogenesis. Depleting cilia disassembly...
During the first five rounds of cell division in mouse embryo, spindles assemble absence centrioles. Spindle formation initiates around chromosomes, but microtubule nucleating process remains unclear. Here we demonstrate that Plk4, a protein kinase known as master regulator centriole formation, is also essential for spindle assembly Depletion maternal Plk4 prevents nucleation and growth microtubules results monopolar formation. This leads to cytokinesis failure and, consequently,...
Drosophila abnormal spindle (asp) mutants exhibit a mitotic metaphase checkpoint arrest with poles, which reflects requirement for Asp the integrity of microtubule organising centres (MTOCs). In male meiosis, absence strong enables asp mutant cells to proceed through anaphase and telophase. However, central region is not correctly organised frequently fail complete cytokinesis. This contrasts meiosis in wild-type males where at late dense array microtubules forms that has localised its...
Centriole and centrosome cycles were examined by indirect immunofluorescence electron microscopy techniques in the early Drosophila embryo. The centrosomes, which are already divided at interphase, appear as compact spheres during prophase metaphase, expand flatten from anaphase to telophase split into two units late telophase. separation starts becomes evident increases Procentrioles following interphase.
AbstractCentrioles are essential for the formation of cilia, flagella and centrosome organization. Abnormalities in structure number many cancers can be associated with aberrant cell division genomic instability.1,2 Canonical centriole duplication occurs coordination cycle, such that a single new "daughter" arises next to each "mother" centriole. If destroyed, or eliminated during development, centrioles form de novo.3-5 Here we discuss our recent data demonstrating molecular pathway...
Male killing, induced by different bacterial taxa of maternally inherited microorganisms, resulting in highly distorted female-biased sex-ratios, is a common phenomenon among arthropods. Some strains the endosymbiont bacteria Wolbachia have been shown to induce this phenotype particular insect hosts. High altitude populations Drosophila bifasciata infected with show selective male killing during embryonic development. However, since was first reported, circa 60 years ago, interaction between...