A5084951688- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- COVID-19 Clinical Research Studies
- Vector-borne infectious diseases
- SARS-CoV-2 detection and testing
- interferon and immune responses
- Virology and Viral Diseases
- Monoclonal and Polyclonal Antibodies Research
- Influenza Virus Research Studies
- Viral Infections and Outbreaks Research
- Olfactory and Sensory Function Studies
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Long-Term Effects of COVID-19
- Computational Drug Discovery Methods
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Pharmacological Receptor Mechanisms and Effects
- Vaccine Coverage and Hesitancy
- Air Quality and Health Impacts
- Biosensors and Analytical Detection
- Bioinformatics and Genomic Networks
Helsinki University Hospital
2017-2024
University of Helsinki
2015-2024
Charité - Universitätsmedizin Berlin
2023-2024
Humboldt-Universität zu Berlin
2023-2024
Freie Universität Berlin
2023-2024
Lund University
2021
Friedrich-Loeffler-Institut
2018
Global Viral
2018
Finnish Institute for Health and Welfare
2018
Johns Hopkins Medicine
2017
The causative agent of coronavirus disease 2019 (COVID-19) is the severe acute respiratory syndrome 2 (SARS-CoV-2). For many viruses, tissue tropism determined by availability virus receptors and entry cofactors on surface host cells. In this study, we found that neuropilin-1 (NRP1), known to bind furin-cleaved substrates, significantly potentiates SARS-CoV-2 infectivity, an effect blocked a monoclonal blocking antibody against NRP1. A mutant with altered furin cleavage site did not depend...
The current outbreak of Zika virus (ZIKV) infection has been associated with an apparent increased risk congenital microcephaly. We describe a case pregnant woman and her fetus infected ZIKV during the 11th gestational week. fetal head circumference decreased from 47th percentile to 24th between 16 20 weeks gestation. RNA was identified in maternal serum at 21 At 19 gestation, substantial brain abnormalities were detected on ultrasonography magnetic resonance imaging (MRI) without presence...
The first case of coronavirus disease (COVID-19) in Finland was confirmed on 29 January 2020. No secondary cases were detected. We describe the clinical picture and laboratory findings 3-23 days since symptoms. SARS-CoV-2/Finland/1/2020 virus strain isolated, genome showing a single nucleotide substitution to reference from Wuhan. Neutralising antibody response appeared within 9 along with specific IgM IgG response, targeting particularly nucleocapsid spike proteins.
Abstract As SARS-CoV-2 has been circulating for over a year, dozens of vaccine candidates are under development or in clinical use. The BNT162b2 mRNA COVID-19 induces spike protein-specific neutralizing antibodies associated with protective immunity. emergence the B.1.1.7 and B.1.351 variants raised concerns reduced efficacy increased re-infection rates. Here we show, that after second dose, sera BNT162b2-vaccinated health care workers (n = 180) effectively neutralize variant D614G...
SUMMARY The causative agent of the current pandemic and coronavirus disease 2019 (COVID-19) is severe acute respiratory syndrome 2 (SARS-CoV-2) 1 . Understanding how SARS-CoV-2 enters spreads within human organs crucial for developing strategies to prevent viral dissemination. For many viruses, tissue tropism determined by availability virus receptors on surface host cells Both SARS-CoV use angiotensin-converting enzyme (ACE2) as a receptor, yet, their tropisms differ 3-5 Here, we found that...
Viruses are one of the major causes acute and chronic infectious diseases thus a contributor to global burden disease. Several studies have shown how viruses evolved hijack basic cellular pathways evade innate immune response by modulating key host factors signalling pathways. A collective view these multiple could advance our understanding virus-host interactions provide new therapeutic perspectives for treatment viral diseases. Here, we performed an integrative meta-analysis elucidate 17...
The newly identified tick-borne Alongshan virus (ALSV), a segmented Jingmen group flavivirus, was recently associated with human disease in China. We report the detection of ALSV RNA Ixodes ricinus ticks south-eastern Finland. Screening sera from patients suspected for encephalitis tick virus-like and antibodies revealed no cases. presence common European warrants further investigations on its role as pathogen.
Severe COVID-19 is characterized by extensive pulmonary complications, to which host immune responses are believed play a role. As the major arm of innate immunity, neutrophils one first cells recruited site infection where their excessive activation can contribute lung pathology. Low-density granulocytes (LDGs) circulating neutrophils, whose numbers increase in some autoimmune diseases and cancer, but poorly acute viral infections. Using flow cytometry, we detected significant LDGs blood...
Repurposing of currently available drugs is a valuable strategy to tackle the consequences COVID-19. Recently, several studies have investigated effect psychoactive on SARS-CoV-2 in cell culture models as well clinical practice. Our aim was expand these and test some compounds against newly emerged variants. Several antidepressants antipsychotic with different primary mechanisms action were tested ACE2/TMPRSS2-expressing human embryonic kidney cells infection by spike protein-dependent...
Novel flaviviruses that are genetically related to pathogenic mosquito-borne (MBFV) have been isolated from mosquitoes in various geographical locations, including Finland. We and characterized another novel virus of this group Finnish collected 2007, designated as Ilomantsi (ILOV). Unlike the MBFV infect both vertebrates mosquitoes, MBFV-related viruses appear be specific similar insect-specific (ISFs). In overview we conclude they differ ISFs antigenically. Phylogenetic analyses separated...
Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) challenge currently available disease 2019 vaccines and monoclonal antibody therapies through epitope change on the receptor binding domain viral spike glycoprotein. Hence, there is a specific urgent need for alternative antivirals that target processes less likely to be affected by mutation, such as membrane fusion step entry into host cell. One antiviral class includes peptide inhibitors, which block formation...
Preventing the biogenesis of disease-relevant proteins is an attractive therapeutic strategy, but attempts to target essential protein factors have been hampered by excessive toxicity. Here we describe KZR-8445, a cyclic depsipeptide that targets Sec61 translocon and selectively disrupts secretory membrane in signal peptide-dependent manner. KZR-8445 potently inhibits secretion pro-inflammatory cytokines primary immune cells highly efficacious mouse model rheumatoid arthritis. A cryogenic...
Prolonged T cell lymphopenia is common in COVID-19, caused by SARS-CoV-2. While the mechanisms of during COVID-19 remain elusive, it especially pronounced a specialized innate-like population called Mucosal Associated Invariant cells (MAITs). MAITs has been suggested to express Angiotensin-Converting Enzyme 2 (ACE2), which well-known cellular receptor for However, still unclear if SARS-CoV-2 can infect or affect MAIT directly. In this study, we performed multicolor flow cytometry on...
ABSTRACT In response to the ongoing COVID-19 pandemic, quest for coronavirus inhibitors has inspired research on a variety of small proteins beyond conventional antibodies, including robust single-domain antibody fragments, i.e., “nanobodies.” Here, we explore potential nanobody engineering in development antivirals and diagnostic tools. Through fusion domains that target distinct binding sites, engineered multimodular constructs neutralize wild-type SARS-CoV-2 Alpha Delta variants at high...
ABT-263 and its structural analogues ABT-199 ABT-737 inhibit B-cell lymphoma 2 (Bcl-2), BCL2L1 long isoform (Bcl-xL) BCL2L2 (Bcl-w) proteins promote cancer cell death. Here, we show that at non-cytotoxic concentrations, these small molecules accelerate the deaths of non-cancerous cells infected with influenza A virus (IAV) or other viruses. In particular, demonstrate altered Bcl-xL interactions Bcl-2 antagonist death (Bad), Bcl-2-associated X protein (Bax), uveal autoantigen coiled-coil...
Viral diseases remain serious threats to public health because of the shortage effective means control. To combat surge viral diseases, new treatments are urgently needed. Here we show that small-molecules, which inhibit cellular anti-apoptotic Bcl-2 proteins (Bcl-2i), induced premature death cells infected with different RNA or DNA viruses, whereas, at same concentrations, no toxicity was observed in mock-infected cells. Moreover, these compounds limited replication and spread....
Primary diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is based on detection virus RNA in nasopharyngeal swab samples. In addition, analysis humoral immunity against SARS-CoV-2 has an important role viral diagnostics and seroprevalence estimates.We developed optimized enzyme immunoassays (EIA) using nucleoprotein (N), S1 receptor binding domain (RBD) the spike protein, N proteins from SARS, Middle East (MERS), 4 low-pathogenic human CoVs. Neutralizing...
SARS-CoV-2 RNA can be detected in respiratory samples for weeks after onset of COVID-19 disease. Therefore, one the diagnostic challenges PCR positive cases is differentiating between acute disease and convalescent phase. The presence nucleocapsid antigen serum plasma patients has been demonstrated previously. Our study aimed to characterize analytical specificity sensitivity an enzyme-linked immunosorbent assay (Salocor Antigen Quantitative Assay Kit© (Salofa Ltd, Salo, Finland)) detection...