A5026198502- Mosquito-borne diseases and control
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Vector-Borne Animal Diseases
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Respiratory viral infections research
- Malaria Research and Control
- COVID-19 Clinical Research Studies
- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Vector-borne infectious diseases
- Vibrio bacteria research studies
- Chronic Myeloid Leukemia Treatments
- vaccines and immunoinformatics approaches
- HIV Research and Treatment
- Phagocytosis and Immune Regulation
- COVID-19 epidemiological studies
- RNA and protein synthesis mechanisms
- SARS-CoV-2 detection and testing
- Animal Disease Management and Epidemiology
- Cancer therapeutics and mechanisms
- Viral Infections and Immunology Research
Institut de Recherche pour le Développement
2012-2024
Aix-Marseille Université
2012-2024
Inserm
2018-2024
Méditerranée Infection Foundation
2012-2022
École des Hautes Études en Santé Publique
2011-2015
Institut de Neurobiologie de la Méditerranée
2006-2010
UK Centre for Ecology & Hydrology
2009
Despite no or limited pre-clinical evidence, repurposed drugs are massively evaluated in clinical trials to palliate the lack of antiviral molecules against SARS-CoV-2. Here we use a Syrian hamster model assess efficacy favipiravir, understand its mechanism action and determine pharmacokinetics. When treatment is initiated before simultaneously infection, favipiravir has strong dose effect, leading reduction infectious titers lungs alleviation disease. Antiviral effect correlates with...
Abstract An outbreak of febrile illness occurred in Gabon 2007, with 20,000 suspected cases. Chikungunya or dengue-2 virus infections were identified 321 patients; 8 patients had documented co-infections. Aedes albopictus was as the principal vector for transmission both viruses.
Here we describe an optimized molecular protocol for the universal detection and identification of flaviviruses. It combines convenient real-time polymerase chain reaction (PCR) format with a broad spectrum flavivirus detection. This assay, based on amplification 269-272 nt (depending tested) region at N terminal end NS5 gene, enabled 51 species 3 tentative species. Sequencing amplicons produced by reverse transcriptase (RT)-PCR permitted reliable taxonomic comparison reference sequences...
The genus Flavivirus contains approximately 70 single-stranded, positive-sense RNA viruses that are mosquito-borne, tick-borne or have no known vector. Two discoveries support previous suggestions of the existence a large number unsampled flaviviruses: (i) new flavivirus, Kamiti River virus, was recently isolated from Kenyan mosquitoes, and (ii) sequences with high similarity to those flaviviruses been found integrated into genome Aedes suggesting past infection virus (or viruses) has yet be...
To generate the most diverse phylogenetic dataset for flaviviruses to date, we determined genomic sequences and relationships of 14 flaviviruses, which 10 are primarily associated with Culex spp. mosquitoes. We analyze these data, in conjunction a comprehensive collection flavivirus genomes, characterize evolutionary biogeographic history unprecedented detail breadth. Based on presumed introduction yellow fever virus into Americas via transatlantic slave trade, extrapolated timescale...
The genus Flavivirus, which contains approximately 70 single-stranded, positive-sense RNA viruses, represents a unique model for studying the evolution of vector-borne disease, as it includes viruses that are mosquito-borne, tick-borne or have no known vector. Both theoretical work and field studies suggest existence large number undiscovered flaviviruses. Recently, first isolation cell fusing agent virus (CFAV) was reported from natural mosquito population in Puerto Rico, sequences related...
We investigated whether small RNA (sRNA) sequenced from field-collected mosquitoes and chironomids (Diptera) can be used as a proxy signature of viral prevalence within range species groups, using sRNAs wild-caught specimens, to inform total deep sequencing samples particular interest. Using this strategy, we adult Anopheles maculipennis s.l. the apparently nearly complete genome one previously undescribed virus related chronic bee paralysis virus, and, pool Ochlerotatus caspius Oc. detritus...
A new virus was isolated from three independent pools of Phlebotomus perniciosus sandflies (Diptera; Psychodidae) trapped in two regions southeastern France, located 90 miles apart. Microscopic, antigenic and genetic analyses indicate that this novel belongs to the genus Phlebovirus family Bunyaviridae. The is designated Massilia since first isolate obtained collected suburban area Marseille. complete genome sequence determined used compare phylogenetic relationships with other...
Duck egg-drop syndrome virus (DEDSV) is a newly emerging pathogenic flavivirus causing avian diseases in China. The infection occurs laying ducks characterized by severe drop egg production with fatality rate of 5–15 %. was found to be most closely related Tembusu (TMUV), an isolate from mosquitoes South-east Asia. Here, we have sequenced and the full-length genomes seven DEDSV strains, including 5′- 3′-non-coding regions (NCRs). We also report for first time ORF sequences TMUV Sitiawan...
Novel flaviviruses that are genetically related to pathogenic mosquito-borne (MBFV) have been isolated from mosquitoes in various geographical locations, including Finland. We and characterized another novel virus of this group Finnish collected 2007, designated as Ilomantsi (ILOV). Unlike the MBFV infect both vertebrates mosquitoes, MBFV-related viruses appear be specific similar insect-specific (ISFs). In overview we conclude they differ ISFs antigenically. Phylogenetic analyses separated...
Abstract Evidence for the tickborne nature of Alkhurma hemorrhagic fever virus (AHFV) is indirect because AHFV has not been detected in arthropods. One Ornithodoros savignyi tick from Saudi Arabia contained RNA. This first direct evidence that a flavivirus and confirms association between human cases tickbite history.
We analysed the complete coding sequences of all recognized species Aedes-borne flavivirus, including previously uncharacterized viruses within yellow fever virus (YFV), Spondweni (SPOV) and dengue (DENV) groups. Two major phylogenetic lineages were revealed: one included YFV Entebbe bat groups, other DENV, SPOV Culex-borne flavivirus This analysis supported previous evidence that flaviviruses have evolved from ancestral viruses. However, topology at junction between these remains complex...
Sandflies are widely distributed around the Mediterranean Basin. Therefore, human populations in this area potentially exposed to sandfly-transmitted diseases, including those caused by phleboviruses. Whilst there substantial data countries located northern part of basin, few available for North Africa. In study, a total 1489 sandflies were collected 2008 Tunisia from two sites, bioclimatically distinct, 235 km apart, and identified morphologically. Sandfly species comprised Phlebotomus...
Following the emergence of SARS-CoV-2, search for an effective and rapidly available treatment was initiated worldwide based on repurposing drugs. Previous reports described antiviral activity certain tyrosine kinase inhibitors (TKIs) targeting Abelson 2 against pathogenic coronaviruses. Imatinib, one them, has more than twenty years safe utilization hematological malignancies. In this context, Imatinib evaluated in clinical trials Covid-19. Here, we present pre-clinical evaluation imatinib...
Abstract To determine whether sandfly fever Sicilian virus (SFSV) is present in Algeria, we tested sandflies for phlebovirus RNA. A sequence closely related to that of SFSV was detected a Phlebotomus ariasi sandfly. Of 60 human serum samples, 3 contained immunoglobulin G against SFSV. These data suggest Algeria.
Sandfly-transmitted phleboviruses, such as Toscana, sandfly fever Sicilian, and Naples, can cause human disease circulate at high rates in Mediterranean countries. Previous studies have also established that viruses other than phleboviruses may be detected isolated from sand flies. The recent detection isolation (in a large variety of mosquito species) insect-only flaviviruses related to cell fusing agent virus has indicated the latter is not an evolutionary remnant but first discovered...
Mosquitoes collected in Finland were screened for flaviviral RNA leading to the discovery and isolation of a novel flavivirus designated Hanko virus (HANKV). Virus characterization, including phylogenetic analysis complete coding sequence, confirmed HANKV as member "insect-specific" (ISF) group. is first this group isolated from northern Europe, therefore European ISF which sequence has been determined. was not transcribed DNA mosquito cell culture, appears atypical an ISF. shared highest...
A new flavivirus, Ecuador Paraiso Escondido virus (EPEV), named after the village where it was discovered, isolated from sand flies (Psathyromyia abonnenci, formerly Lutzomyia abonnenci) that are unique to New World. This represents first fly-borne flavivirus identified in EPEV exhibited a typical genome organization. Nevertheless, maximum pairwise amino acid sequence identity with currently recognized flaviviruses 52.8%. Phylogenetic analysis of complete coding showed distinct clade which...
Abstract Jingmen tick virus (JMTV) is a recently discovered segmented RNA virus, closely related to flaviviruses. It was identified for the first time in 2014, China and subsequently Brazil. Following this discovery, JMTV-related sequences have been arthropods, vertebrates (including humans), plants, fungus environmental samples from Asia, America, Africa, Europe Oceania. Several studies suggest an association between these flavi-like viruses, termed jingmenviruses, febrile illness humans....