A5068537765- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS drug development and treatment
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- interferon and immune responses
- Virus-based gene therapy research
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Cutaneous lymphoproliferative disorders research
- RNA Interference and Gene Delivery
- Lymphoma Diagnosis and Treatment
- Viral gastroenteritis research and epidemiology
- HIV-related health complications and treatments
- COVID-19 Clinical Research Studies
- HIV/AIDS Research and Interventions
- Viral Infections and Immunology Research
- Protein Kinase Regulation and GTPase Signaling
- RNA and protein synthesis mechanisms
- CAR-T cell therapy research
- Cell Adhesion Molecules Research
- T-cell and Retrovirus Studies
- Mosquito-borne diseases and control
- Herpesvirus Infections and Treatments
- Hepatitis C virus research
University of Helsinki
2016-2025
Helsinki University Hospital
2016-2025
Hospital District of Helsinki and Uusimaa
2021-2024
University of Zurich
2021-2023
Finnish Institute for Health and Welfare
2021
Helsinki Art Museum
2014
Tampere University
1998-2011
Tampere University of Applied Sciences
2000-2011
University of Ljubljana
2011
University of California, San Francisco
2011
Designing smarter anticancer T cells Biological signaling systems can exhibit a large, nonlinear—or “ultrasensitive”—response, which would be useful to engineer into therapeutic allow for better discrimination between cancer and normal tissues. Hernandez-Lopez et al. modified human using two-step mechanism that allowed them kill expressing large amounts of marker protein but not small amount the same protein. A first synthetic receptor recognized antigen with low affinity. That signaled...
To study the basis of cellular latency human immunodeficiency virus (HIV), we have used a recombinant luciferase-encoding HIV (HXB-Luc) to superinfect nonproductively HIV-1-infected leukemic cell lines. HXB-Luc contains Photinus pyralis luciferase gene in place nef and provides highly sensitive, simple assay for infection expression. circumvent any superinfection block latently infected cells, also generated viruses pseudotyped with murine leukemia amphotropic envelope (HXB-Luc:ampho). The...
Among the four non-structural proteins of alphaviruses function nsP3 is least well understood. NsP3 a component viral replication complex, and composed conserved aminoterminal macro domain implicated in RNA synthesis, poorly carboxyterminal region. Despite lack overall homology we noted proline-rich sequence motif shared by many alphaviral proteins, found it to serve as preferred target site for Src-homology 3 (SH3) domains amphiphysin-1 -2. Nsp3 Semliki Forest (SFV), Sindbis (SINV),...
Antiretroviral therapy (ART) efficiently suppresses HIV replication but immune activation and low CD4 T cell counts often persist. The underlying mechanism of this ART-resistant pathogenesis is not clear. We observed that levels plasma extracellular vesicles (pEV) are strongly elevated in infection do decline during ART. Surprisingly, these contained the viral accessory proteins Nef Vpu, which assumed to be expressed under efficient ART, as well pro-inflammatory effectors, including...
Repurposing of currently available drugs is a valuable strategy to tackle the consequences COVID-19. Recently, several studies have investigated effect psychoactive on SARS-CoV-2 in cell culture models as well clinical practice. Our aim was expand these and test some compounds against newly emerged variants. Several antidepressants antipsychotic with different primary mechanisms action were tested ACE2/TMPRSS2-expressing human embryonic kidney cells infection by spike protein-dependent...
The hepatitis C virus nonstructural 5A (NS5A) protein is a pleiotropic phosphoprotein that has been shown to associate with wide variety of cellular signaling proteins. Of particular interest the observation highly conserved C-terminal Class II polyproline motif within NS5A mediated association Src homology 3 domains members family tyrosine kinases and mitogenic adaptor Grb2 (A. Macdonald, K. Crowder, A. Street, C. McCormick, M. Harris, submitted for publication). In this study, we analyzed...
To address the significance of human immunodeficiency virus (HIV) replication in peripheral blood mononuclear cells (PBMCs), we have used reverse transcriptase-initiated PCR to measure HIV-1 mRNA expression PBMC specimens collected from a cohort HIV-infected individuals during long-term prospective study. We found dramatic differences HIV among with very similar clinical and laboratory indices, this variation strongly correlated future course disease. No evidence viral was detected PBMCs...
ABSTRACT MicroRNAs have emerged as important players in tissue-specific mammalian gene regulation and also been exploited experimental targeting of expression. We constructed a recombinant adenovirus that contains sequences complementary to the liver-specific microRNA 122 (miR122) 3′ untranslated region E1A gene. In Huh7 cells, which resemble normal hepatocytes expressing high levels miR122, this feature resulted strongly reduced mRNA protein. This property allowed us generate novel was...
Enterohemorrhagic Escherichia coli O157:H7 translocates 2 effectors to trigger localized actin assembly in mammalian cells, resulting filamentous “pedestals.” One effector, the translocated intimin receptor (Tir), is plasma membrane and clustered upon binding bacterial outer protein intimin. The second, proline-rich effector EspF U (aka TccP) activates nucleation-promoting factor WASP/N-WASP, recruited sites of attachment by a mechanism dependent on an Asn-Pro-Tyr (NPY 458 ) sequence Tir...
The ongoing coronavirus disease 2019 (COVID-19) pandemic has seen an unprecedented increase in the demand for rapid and reliable diagnostic tools, leaving many laboratories scrambling resources. We present a fast simple assay principle antigen detection demonstrate its functionality by detecting severe acute respiratory syndrome 2 (SARS-CoV-2) antigens nasopharyngeal swabs. method is based on of SARS-CoV-2 nucleoprotein (NP) S protein (SP) via time-resolved Förster resonance energy transfer...
Abstract Introduction Plasma proteins affect biological processes and are common drug targets but their role in the development of Alzheimer's disease related dementias remains unclear. We examined associations between 4953 plasma cognitive decline risk dementia two cohort studies with 20‐year follow‐ups. Methods In Whitehall II prospective study were measured using SOMAscan technology. Cognitive performance was tested five times over 20 years. Linkage to electronic health records identified...
Traditional Western blotting is one of the most used analytical techniques in biological research. However, it can be time-consuming and suffer from a lack reproducibility. Consequently, devices with different degrees automation have been developed. These include semi-automated fully automated that replicate all stages downstream sample preparation, including size separation, immunoblotting, imaging, analysis. We directly compared traditional two systems, iBind™ Flex, which system designed...
Nef is a lentiviral protein involved in pathogenesis of AIDS, but its molecular mechanisms action remain incompletely understood. Here we report novel effect on lymphocyte signaling, which mediated via T cell receptor (TCR)-independent contribution to induction nuclear factor activated cells (NFAT), transcription that plays central role coordinating activation. Expression did not significantly alter the basal level NFAT activity Jurkat nor increased following stimulation by anti-CD3 or +...