A5043040909- Trauma and Emergency Care Studies
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Neuropeptides and Animal Physiology
- Abdominal Trauma and Injuries
- Blood transfusion and management
- Bacterial Genetics and Biotechnology
- Cardiac Arrest and Resuscitation
- Emergency and Acute Care Studies
- Neurotransmitter Receptor Influence on Behavior
- Trauma Management and Diagnosis
- Receptor Mechanisms and Signaling
- Marine Sponges and Natural Products
- Alkaloids: synthesis and pharmacology
- Synthetic Organic Chemistry Methods
- Venous Thromboembolism Diagnosis and Management
- Microbial Natural Products and Biosynthesis
- Traffic and Road Safety
- Sepsis Diagnosis and Treatment
- Injury Epidemiology and Prevention
- Pharmacological Receptor Mechanisms and Effects
- Hemophilia Treatment and Research
- Vitamin K Research Studies
- Enzyme Production and Characterization
- Traditional and Medicinal Uses of Annonaceae
- Alcoholism and Thiamine Deficiency
Witten/Herdecke University
2017-2024
Kliniken der Stadt Köln
2017-2023
Lungenklinik Köln-Merheim
2019
University Hospital Bonn
2018
University of Kansas
2011-2016
Sanofi (Germany)
2015
Rockefeller University
2012
Forschungszentrum Jülich
2006-2010
Summary Deficiencies of natural anticoagulant proteins including antithrombin (AT), protein C (PC) and S (PS) are important causes inherited thrombophilia. This study aimed to report on the practical experience gained in performing genetic analyses a large cohort patients with AT, PC PS deficiencies relate this knowledge clinical application. We genotyped 709 unrelated AT (231), (234) (244) referred us by physicians throughout Germany. Mutations were detected direct sequencing multiplex...
Microbial natural products are a rich source of bioactive molecules to serve as drug leads and/or biological tools. We investigated little-explored myxobacterial genus, Nannocystis sp., and discovered novel 21-membered macrocyclic scaffold that is composed tripeptide polyketide part with an epoxyamide moiety. The relative absolute configurations the nine stereocenters was determined by NMR spectroscopy, molecular dynamics calculations, chemical degradation, X-ray crystallography. compound,...
As part of our continuing efforts toward more fully understanding the structure−activity relationships neoclerodane diterpene salvinorin A, we report synthesis and biological characterization unique cycloadducts through [4+2] Diels−Alder cycloaddition. Microwave-assisted methods were developed successfully employed, aiding in functionalizing chemically sensitive A scaffold. This demonstrates first reported results for both cycloaddition furan ring functionalization via microwave-assisted...
VKORC1 and VKORC1L1 are enzymes that both catalyze the reduction of vitamin K2,3-epoxide via K quinone to hydroquinone. is key enzyme classical cycle by which K-dependent (VKD) proteins γ-carboxylated hepatic γ-glutamyl carboxylase (GGCX). In contrast, paralog enzyme, VKORC1L1, chiefly responsible for antioxidative function prevent damage intracellular reactive oxygen species. To investigate tissue-specific 2,3-epoxide reductase (VKOR) enzymes, we quantified mRNA levels VKORC1, GGCX, NQO1...
While the incidence and aspects of pneumonia in ICU patients has been extensively discussed literature, studies on occurrence severely injured are rare. The aim present study is to elucidate factors associated with thoracic trauma. Level-I University Trauma Centres TraumaRegister DGU®. A total 1162 adult trauma documented DGU® (TR-DGU) were included this study. Demographic data, injury severity, duration mechanical ventilation (MV), stay, pneumonia, bronchoalveolar lavage, aspiration,...
We have identified 1,135 haemophilia A patients with missense mutations associated mild (46%), moderate (22%), severe (16%), and mixed phenotypes (11%). Altogether, we detected 374 different of which 195 are not listed in the HAMSTeRS database. While strongly underrepresented within factor VIII (FVIII) B-domain, they evenly distributed throughout entire F8 cDNA sequence. Only 36 (5%) 720 known inhibitor status showed an association formation. Inhibitor prevalence was four-fold higher for...
After severe polytrauma the dynamic process of coagulation may deteriorate towards a trauma-induced coagulopathy (TIC) promoting dramatic increase in morbidity and mortality. Recent evidence suggests that microparticles (MPs) play pivotal role at interface between cellular plasmatic systems. However, impact MPs on functional has not been clarified yet setting traumatic injuries. We assessed temporal patterns circulating MP concentrations including their origin context clinical presentation...
External factors following trauma and iatrogenic intervention influence blood coagulation particularly clot formation. In particular, three external (in detail dilution via uncritical volume replacement, acidosis hypothermia), in combination, referred to as the "lethal triad", substantially aggravate hypocoagulative state after trauma. Contribution of these resulting especially their on primary haemostasis has still not been investigated systematically. This study aims assess this...
Active blood-brain barrier mechanisms, such as the major efflux transporter P-glycoprotein (mdr1), modulate in vivo/central nervous system (CNS) effects of many pharmacological agents, whether they are used for nonmedical reasons or pharmacotherapy. The powerful, widely available hallucinogen salvinorin A (from plant <i>Salvia divinorum</i>) is a high-efficacy, selective κ-opioid agonist and displays fast-onset behavioral (e.g., within 1 min administration) relatively short duration action....
Abstract Background Second medical opinions (SOs) can assist patients in making informed treatment decisions and improve the understanding of their diagnosis. In Germany, there are different approaches to obtain a structured SO procedure: programs by health insurers SOs according Directive. Through direct survey population, we aimed assess how should be provided fulfil patients’ needs. Methods A stratified sample 9990 adults (≥18 years) living federal states Berlin Brandenburg (Germany) were...
Death from uncontrolled trauma haemorrhage and subsequent trauma-induced coagulopathy (TIC) is potentially preventable. Point-of-care devices such as rotational thromboelastometry (ROTEM®) are advocated to detect haemostatic derangements more rapidly than conventional laboratory diagnostics. Regarding reductions in RBC transfusion, the use of ROTEM has been described being efficient associated with positive outcomes several studies. The effect was assessed on three different outcome...
Background/Objective: This prospective, multicenter observational cohort study was carried out in 12 trauma centers Germany and Switzerland. Its purpose to evaluate the rate of undertriage, as well potential consequences, relate these with different Trauma Team Activation Protocols (TTA-Protocols), this has not been done before Germany. Methods: Each center collected data during a three-month period between December 2019 February 2021. All participating hospitals are certified supra-regional...
Salvinorin A is a kappa opioid agonist and the principal psychoactive constituent of Salvia divinorum plant, which has been used for hallucinogenic effects. Previous research on salvinorin pharmacokinetics likely underestimated plasma levels typically resulting from doses administered due to inefficient vaporization not collecting samples during peak drug Six healthy adults inhaled single high dose vaporized ( n = 4, 21 mcg/kg; 2, 18 mcg/kg). Participant- monitor-rated effects were assessed...
Abstract Microbial natural products are a rich source of bioactive molecules to serve as drug leads and/or biological tools. We investigated little‐explored myxobacterial genus, Nannocystis sp., and discovered novel 21‐membered macrocyclic scaffold that is composed tripeptide polyketide part with an epoxyamide moiety. The relative absolute configurations the nine stereocenters was determined by NMR spectroscopy, molecular dynamics calculations, chemical degradation, X‐ray crystallography....
Detailed and decisive information about the patients' coagulation status is important in various emergency situations. Conventional global testing strategies are often used to provide a quick overview, but several limitations particularly trauma setting well described. With introduction of direct oral anticoagulations (DOACs), milestone for disease entities resulting overall improved outcomes could be reached, at same time providing new diagnostic challenges situation. As an alternative...
Multiple trauma patients with severe chest are at increased risk for tracheostomy. While the factors associated need tracheostomy well established in general critical care population, they have not yet been validated a cohort of suffering thoracic trauma. This retrospective study analysed data on aged 18 years or older who were admitted to one six participating academic level I centres multiple injuries, including (AISThorax ≥ 3) between 2010 and 2014. A multivariable binary regression was...