A5007225666- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- Advanced Control Systems Optimization
- Amino Acid Enzymes and Metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Food composition and properties
Forschungszentrum Jülich
2016-2024
Abstract Anthranilate and its derivatives are important basic chemicals for the synthesis of polyurethanes as well various dyes food additives. Today, anthranilate is mainly chemically produced from petroleum‐derived xylene, but this shikimate pathway intermediate could be also obtained biotechnologically. In study, Corynebacterium glutamicum was engineered microbial production a carbon source mixture glucose xylose. First, feedback‐resistant 3‐deoxy‐arabinoheptulosonate‐7‐phosphate synthase...
Metaraminol is an active pharmaceutical ingredient for hypotension treatment and can serve as a precursor other bioactive compounds. The production of metaraminol was shown previously in enzymatic cascade consisting carboligation transamination step using the oil-based amine donor isopropylamine. Alternatively, renewable l-alanine be used. By iterative strain process engineering, microbial pyruvate with Corynebacterium glutamicum one-pot fermentation achieved. subsequent biotransformation...
Corynebacterium glutamicum can grow on d-xylose as sole carbon and energy source via the five-step Weimberg pathway when pentacistronic xylXABCD operon from Caulobacter crescentus is heterologously expressed. More recently, it could be demonstrated that C. wild type accumulates intermediate d-xylonate cultivated in presence of d-xylose. Reason for this activity endogenous dehydrogenase IolG, which also oxidize This raised question whether additional enzymes contribute to catabolization...
Abstract Background Lignocellulosic biomass is the most abundant raw material on earth. Its efficient use for novel bio‐based materials essential an emerging bioeconomy. Possible building blocks such are key TCA‐cycle intermediates α‐ketoglutarate and succinate. These organic acids have a wide range of potential applications, particularly in as monomers established or biopolymers. Recently, Corynebacterium glutamicum was successfully engineered evolved towards improved utilization d ‐xylose...
3,4-Dihydroxybenzoate (protocatechuate, PCA) is a phenolic compound naturally found in edible vegetables and medicinal herbs. PCA of high interest the chemical industry has wide potential for pharmaceutical applications. We designed constructed novel Corynebacterium glutamicum strain to enable efficient utilization d-xylose microbial production PCA. Shake flask cultivation engineered showed maximum titer 62.1 ± 12.1 mM (9.6 1.9 g L-1 ) from as primary carbon energy source. The corresponding...
α-ketoglutarate (AKG) is a valuable dicarboxylic acid with multiple applications in the food, pharmaceutical, and chemical industries. Its synthesis associated toxic by-products, low specificity, high energy input. To create more environmentally friendly sustainable alternative, microbial production process for AKG was developed. Four potential producer strains were generated by metabolic engineering of Corynebacteriumglutamicum characterized on defined glucose/sucrose media as well...
Abstract Anthranilate and its derivative aniline are important basic chemicals for the synthesis of polyurethanes as well various dyes food additives. Today, is mainly chemically produced from petroleum-derived benzene, but it could be also obtained more sustainably by decarboxylation microbially shikimate pathway intermediate anthranilate. In this study, Corynebacterium glutamicum was engineered microbial production anthranilate a carbon source mixture glucose xylose. First,...
3,4-Dihydroxybenzoate (protocatechuate, PCA) is a phenolic compound naturally found in edible vegetables and medicinal herbs. PCA of interest the chemical industry as building block for novel polymers has wide potential pharmaceutical applications due to its antioxidant, anti-inflammatory, antiviral properties. In present study, we designed constructed Corynebacterium glutamicum strain enable efficient utilization d -xylose microbial production PCA. The engineered showed maximum titer 62.1 ±...