A5080163890- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Fungal and yeast genetics research
- Plant Disease Resistance and Genetics
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Advanced Electron Microscopy Techniques and Applications
- Bacteriophages and microbial interactions
- Electron and X-Ray Spectroscopy Techniques
- Heat shock proteins research
- Yeasts and Rust Fungi Studies
- RNA Research and Splicing
University of Regensburg
2019-2024
Abstract The TATA-binding protein (TBP) and a transcription factor (TF) IIB-like are important constituents of all eukaryotic initiation complexes. reason for the emergence strict requirement additional Bdp1 in RNA polymerase (RNAP) III system, however, remained elusive. A poorly studied aspect this context is effect DNA strain arising from compaction transcriptional activity on complex formation. We made use origami-based force clamp to follow assembly human complexes RNAP II systems at...
RNA polymerase (Pol) I transcribes the ribosomal precursor in all eukaryotes. The mechanisms 'activation by cleft contraction' and 'hibernation dimerization' are unique to regulation of this enzyme, but structure-function analysis is limited baker's yeast. To understand whether such strategies specific model organism or conserved among species, we solve three cryo-EM structures Pol from Schizosaccharomyces pombe different functional states. Comparative structural models derived...
Abstract Archaeal transcription is carried out by a multi-subunit RNA polymerase (RNAP) that highly homologous in structure and function to eukaryotic RNAP II. Among the set of basal factors, only Spt5 found all domains life, but has been shaped during evolution, which also reflected heterodimerization with Spt4 Archaea Eukaryotes. To unravel mechanistic basis Spt4/5 Archaea, we performed structure-function analyses using archaeal transcriptional machinery Pyrococcus furiosus (Pfu). We...
Transcription of the ribosomal RNA precursor by polymerase (Pol) I is a major determinant cellular growth, and dysregulation observed in many cancer types. Here, we present purification human Pol from cells carrying genomic GFP fusion on largest subunit allowing structural functional analysis enzyme across species. In contrast to yeast, carries single-subunit stalk, vitro transcription indicates reduced proofreading activity. Determination cryo-EM reconstruction close-to-native state...
Abstract Recent technological progress revealed new prospects of high-resolution structure determination macromolecular complexes using cryo-electron microscopy (cryo-EM). In the field RNA polymerase (Pol) I research, a number cryo-EM studies contributed to understanding highly specialized mechanisms underlying transcription ribosomal genes. Despite broad applicability method itself, preparation samples for data collection can be challenging. Here, we describe strategies purification and...
Abstract Archaeal transcription is carried out by a multi-subunit RNA polymerase (RNAP) that highly homologous in structure and function to eukaryotic RNAP II. Among the set of basal factors, only Spt5 found all domains life but has been shaped during evolution, which also reflected heterodimerization with Spt4 Archaea Eukaryotes. To unravel mechanistic basis Spt4/5 Archaea, we performed structure-function analyses using archaeal transcriptional machinery Pyrococcus furiosus ( Pfu ). We...
Abstract The TATA-binding protein (TBP) and a transcription factor (TF) IIB-like compound the fundamental core of all eukaryotic initiation complexes. reason for emergence strict requirement additional intiation Bdp1, which is unique to RNA polymerase (RNAP) III sytem, however, remained elusive. A poorly studied aspect in this context effect DNA strain, that arises from compaction transcriptional activity, on efficiency complex formation. We made use new nanotechnological tool –...
Abstract Transcription of the ribosomal RNA precursor by polymerase (Pol) I is a major determinant cellular growth and dysregulation observed in many cancer types. Here, we present purification human Pol from cells carrying genomic GFP-fusion on largest subunit allowing structural functional analysis enzyme across species. In contrast to yeast, carries single-subunit stalk vitro transcription indicates reduced proofreading activity. Determination cryo-EM reconstruction close-to-native state...