A5039186820- Legume Nitrogen Fixing Symbiosis
- Nematode management and characterization studies
- Plant Parasitism and Resistance
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Advanced biosensing and bioanalysis techniques
- Malaria Research and Control
- Bacterial Genetics and Biotechnology
- Plant Molecular Biology Research
- Plant Reproductive Biology
- DNA and Nucleic Acid Chemistry
- Garlic and Onion Studies
- Cassava research and cyanide
- Genomic variations and chromosomal abnormalities
- Plant Disease Resistance and Genetics
- Polyamine Metabolism and Applications
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Nephrotoxicity and Medicinal Plants
- Escherichia coli research studies
- Immunodeficiency and Autoimmune Disorders
- Plant Pathogens and Fungal Diseases
- Bacterial Identification and Susceptibility Testing
Huazhong Agricultural University
2024
Chinese Academy of Agricultural Sciences
2024
Ministry of Agriculture and Rural Affairs
2024
Agricultural Genomics Institute at Shenzhen
2024
Kumamoto University
2014-2023
Wakunaga (Japan)
1987-1996
Teikyo University
1992
Clearing techniques eliminate factors that interfere with microscopic observation, including light scattering and absorption by pigments cytoplasmic components. The allow fluorescence-based detailed analyses of materials characterization the three-dimensional structure organs. We describe a simple rapid clearing imaging method, termed 'TOMEI' (Transparent plant Organ MEthod for Imaging), which enables observation intact This method involves reagent containing 2,2′-thiodiethanol....
In order to identify methicillin-resistant staphylococci from clinical sources with ease and reliability, enzymatic detection of polymerase chain reaction (ED-PCR) was applied. ED-PCR is based on the capture amplified products via biotin-streptavidin affinity an incorporated hapten in enzyme-linked antibody. all species, a 150-bp fragment mecA gene targeted for ED-PCR. After PCR performed pair biotin dinitrophenol 5'-labeled primers, mixture applied microtiter well precoated streptavidin....
Developmental plasticity is one of the most striking features plant morphogenesis, as plants are able to vary their shapes in response environmental cues. Biotic or abiotic stimuli often promote organogenesis events not observed under normal growth conditions. Root-knot nematodes (RKNs) known parasitize multiple species rooting and induce characteristic tissue expansion called galls root-knots on roots hosts by perturbing cellular machinery. Galls contain giant cells (GCs) neighboring cells,...
Lateral roots are important for a wide range of processes, including uptake water and nutrients. The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION-RELATED (CLE) 1 ~ 7 peptide family their cognate receptor CLV1 have been shown to negatively regulate lateral root formation under low-nitrate conditions. However, little is known about how CLE signaling regulates formation. A persistent obstacle in research functional redundancies, which makes analyses difficult. To address this problem, we generate...
The influence of garlic extract on the chronic toxicity test were examined orally in Wistar rats for 6 months. There no toxic symptoms due to even at dose level 2000 mg/kg 5 times a week during High did not inhibit body weight gain, while food consumption decreased slightly nutritional effects it both male and female rats. significant differences urinary, hematological serological examinations compared each groups. In histopathological findings, signs observed any tissues organs examined.
Abstract F‐4, a protein fraction isolated from aged garlic extract strongly stimulates mice peritoneal macrophages in terms of glucose utilization and exhibits cytostatic activity vitro . F‐4 also has mitogenic on spleen cells. In vivo , induced the stimulation carbon clearance mice. No obvious activities were observed low molecular these tests, but weak effects found fructan (F‐3).
Root-knot nematodes (Meloidogyne incognita) are one of the most economically damaging nematodes; thus it is important to know molecular mechanisms involved in phases nematode infection.To use this as a material for research, we must constantly prepare sufficient numbers nematodes, preferably sterilized.Here have developed alternative methods culture, sterilization, and inoculation root-knot that especially suitable Arabidopsis thaliana Heynh host plants.Nematol.Res. 45(1), 45-49(2015).
Abstract In plants, growth–defense tradeoffs are essential for optimizing plant performance and adaptation under stress conditions, such as pathogen attack. Root-knot nematodes (RKNs) cause severe economic losses in many crops worldwide, although little is known about the mechanisms that control growth defense responses during nematode Upon investigation of Arabidopsis thaliana infected with RKN ( Meloidogyne incognita ), we observed atypical transcription factor DP-E2F-like 1 DEL1 )...
Plants use many long-distance and systemic signals to modulate growth development, as well respond biotic abiotic stresses. Parasitic nematodes infect host plant roots cause severe damage crop plants. However, the molecular mechanisms that regulate parasitic nematode infections are still unknown. Here, we show root-knot (RKNs), Meloidogyne incognita, CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION (CLE)-CLV1 signaling module promote infection progression. deficient in CLE pathway enhanced RKN...
Abstract We have developed a colorimetric assay, “microtiter plate-hybridization”, for the detection of malaria parasites Plasmodium falciparum and P. vivax in human blood, which target DNA sequences (18s small subunit ribosomal RNA gene) amplified by polymerase chain reaction (PCR) are hybridized with species-specific probes immobilized on microtiter well. This assay system was tested Guadalcanal, Solomon Islands, where is highly endemic. obtained blood samples finger puncture from 130...
Abstract Lateral (L‐) flagella‐having vibrios were classified into 13 H‐serogroups (flagellar antigen serogroups) by means of H‐agglutination test. Vibrio parahaemolyticus was 3 serogroups, HL1 to 3. V. alginolyticus and harveyi 5 respectively, but 2 those serogroups common the both species. fluvialis furnissii constituted a same serogroup, HL8. Cross‐reactivity between each serogroup not observed in test, although some cross‐reactivity gel diffusion Furthermore, similarity DNA sequence...
Meloidogyne incognita is one of the most detrimental root-knot nematode pests in world because its wide range hosts, infecting almost all plant species. Following infection, this induces gall formation root. We have found that these induced galls turn green tomato roots exposed to light. This greening was light-dependent and inhibited by auxin treatment. Chlorophyll detected galls, chloroplasts were also observed region. Expression chlorophyll a / b-binding protein-4 gene upregulated galls....