A5045957255- RNA and protein synthesis mechanisms
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Microtubule and mitosis dynamics
- Data Management and Algorithms
- 3D Printing in Biomedical Research
- Web Data Mining and Analysis
- Advanced Database Systems and Queries
- IL-33, ST2, and ILC Pathways
- CRISPR and Genetic Engineering
- Nanopore and Nanochannel Transport Studies
- Cardiomyopathy and Myosin Studies
- Immune Cell Function and Interaction
- Bacterial Genetics and Biotechnology
- Cellular Mechanics and Interactions
- Natural Language Processing Techniques
- Alzheimer's disease research and treatments
- Mitochondrial Function and Pathology
- Bacteriophages and microbial interactions
Japan Science and Technology Agency
2008-2024
The University of Tokyo
2015-2024
Tokyo University of Science
2022-2023
Stanford University
2006-2015
RIKEN Center for Integrative Medical Sciences
2011-2013
KDDI Research (Japan)
2013
CF Industries Holdings (United States)
2013
CLS Technology (Norway)
2013
Sumitomo Electric Industries (United States)
2010-2013
KDDI (Japan)
2008
The advent of image-activated cell sorting and imaging-based picking has advanced our knowledge exploitation biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure molecular landscape cell, which critical limitations. Here we demonstrate Raman by directly probing chemically specific intracellular vibrations via ultrafast multicolor stimulated scattering (SRS) microscopy phenotyping. Specifically,...
Abstract By virtue of the combined merits flow cytometry and fluorescence microscopy, imaging (IFC) has become an established tool for cell analysis in diverse biomedical fields such as cancer biology, microbiology, immunology, hematology, stem biology. However, performance utility IFC are severely limited by fundamental trade-off between throughput, sensitivity, spatial resolution. Here we present optomechanical method that overcomes virtually freezing motion flowing cells on image sensor...
The upgraded version of intelligent image-activated cell sorting (iIACS) has enabled higher-throughput and more sensitive image-based single live cells from heterogeneous populations.
Kinesin-1, the founding member of kinesin superfamily proteins, is known to use only a subset microtubules for transport in living cells. This biased proposed as guidance cue polarized neurons, but underlying mechanisms are still poorly understood. Here, we report that kinesin-1 binding changes microtubule lattice and promotes further binding. high-affinity state requires nucleotide-free state. Microtubules return initial low-affinity by washing out or non-hydrolyzable ATP analogue AMPPNP...
Droplet microfluidics has become a powerful tool in precision medicine, green biotechnology, and cell therapy for single-cell analysis selection by virtue of its ability to effectively confine cells. However, there remains fundamental trade-off between droplet volume sorting throughput, limiting the advantages small droplets (<10 pl) that are incompatible with long-term maintenance growth most We present sequentially addressable dielectrophoretic array (SADA) sorter overcome this problem....
Kinesin is a motor protein that transports organelles along microtubule toward its plus end by using the energy of ATP hydrolysis. To clarify nucleotide-dependent binding mode, we measured unbinding force for one-headed kinesin heterodimers in addition to conventional two-headed homodimers under several nucleotide states. We found both weak and strong state exist each head corresponding small large force, respectively; is, ADP nucleotide-free adenosine 5'-[beta,gamma-imido]triphosphate Model...
The purpose of this study is to propose Context-Aware Support Vector Machine (C-SVM) for application in a context-dependent recommendation system. It important consider users' contexts information as preference change with context. However, currently there are few methods which take into account (e.g. time, place, the situation and so on). Thus, we extend functionality Machines (SVM), popular classifier method used between two classes, by adding axes context feature space order We then...
Inferring antibiotic mechanisms on translation through static structures has been challenging, as biological systems are highly dynamic. Dynamic single-molecule methods also limited to few simultaneously measurable parameters. We have circumvented these limitations with a multifaceted approach investigate three structurally distinct aminoglycosides that bind the aminoacyl-transfer RNA site (A site) in prokaryotic 30S ribosomal subunit: apramycin, paromomycin, and gentamicin. Using several...
Abstract Necroptosis is a regulated form of necrosis that depends on receptor-interacting protein kinase (RIPK)3 and mixed lineage domain-like (MLKL). While danger-associated molecular pattern (DAMP)s are involved in various pathological conditions released from dead cells, the underlying mechanisms not fully understood. Here we develop fluorescence resonance energy transfer (FRET) biosensor, termed SMART (a sensor for MLKL activation by RIPK3 based FRET). composed fragment monitors...
We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic (cytRNA) from single cell enables single-cell integrated nucRNA cytRNA-sequencing (SINC-seq). SINC-seq constructs two individual RNA-seq libraries, cytRNA, per cell, quantifies gene expression in the subcellular compartments, combines them to create novel data. Leveraging SINC-seq, we discover distinct natures of correlation among cytRNA reflect transient physiological state cells. These data...
Update management of XML documents is an increasingly important research issue in databases, because contents evolve as time goes by. Even though, databases should be able to effectively process queries well updates on the documents. We propose a robust node-numbering scheme for named QRS (quartering-regions scheme). In QRS, reefs (regions expressed by floating-point numbers), variant regions, are used expressing node-numbers. Reefs almost compatible and thus they can detecting...
Article12 April 2018Open Access Source DataTransparent process Real-time observation of flexible domain movements in CRISPR–Cas9 Saki Osuka Department Biological Sciences, Graduate School Science, The University Tokyo, Japan Search for more papers by this author Kazushi Isomura Shohei Kajimoto Tomotaka Komori Hiroshi Nishimasu Tomohiro Shima Corresponding Author [email protected] orcid.org/0000-0001-7972-4965 Osamu Nureki orcid.org/0000-0003-1813-7008 Sotaro Uemura Information Osuka1,2,...
For most of the index structures for XML data proposed so far, updating is a problem, because an element's coordinates are expressed using absolute values. Due to structural relationship among elements in documents, we have re-compute these values if content source updated. The reconstruction requires large portion files, which causes serious especially when updated frequently. In this paper, propose indexing structure scheme based on relative region that can effectively deal with update...