A5034837543- Cell Image Analysis Techniques
- COVID-19 Clinical Research Studies
- Platelet Disorders and Treatments
- 3D Printing in Biomedical Research
- Single-cell and spatial transcriptomics
- Inflammatory Biomarkers in Disease Prognosis
- Microfluidic and Bio-sensing Technologies
- Heparin-Induced Thrombocytopenia and Thrombosis
- Systemic Lupus Erythematosus Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Image Processing Techniques and Applications
- Optical Coherence Tomography Applications
- Asthma and respiratory diseases
- COVID-19 and healthcare impacts
- DNA and Biological Computing
- Cell Adhesion Molecules Research
- Advanced biosensing and bioanalysis techniques
- Genetics, Bioinformatics, and Biomedical Research
- Allergic Rhinitis and Sensitization
- Long-Term Effects of COVID-19
- Photoacoustic and Ultrasonic Imaging
- Immune Cell Function and Interaction
- Digital Imaging for Blood Diseases
- Advanced Fluorescence Microscopy Techniques
- Mast cells and histamine
CYBO (Japan)
2019-2024
The University of Tokyo
1983-2021
Japan Science and Technology Agency
2017-2020
Kawaguchi Junior College
2017
Radiation Effects Research Foundation
2007
Tokyo University of the Arts
2004
Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, metabolic engineering. It enables measurements statistical analyses chemical, structural, morphological phenotypes numerous living cells to provide systematic insights into biological processes. However, its utility constrained by requirement fluorescent labeling for phenotyping. Here we present...
The advent of image-activated cell sorting and imaging-based picking has advanced our knowledge exploitation biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure molecular landscape cell, which critical limitations. Here we demonstrate Raman by directly probing chemically specific intracellular vibrations via ultrafast multicolor stimulated scattering (SRS) microscopy phenotyping. Specifically,...
The MCP-1 (monocyte chemoattractant protein-1)/CCR2 (CC motif chemokine receptor-2) pathway may play a role in macrophage infiltration into obese adipose tissue. Here we investigated the of CCR2 recruitment bone marrow-derived macrophages tissue vitro and vivo. Using TAXIScan device, which can measure quantitatively directionality velocity cell migration at time lapse intervals vitro, demonstrated that marrow cells (BMCs) from wild type mice migrate directly toward or culture medium...
The upgraded version of intelligent image-activated cell sorting (iIACS) has enabled higher-throughput and more sensitive image-based single live cells from heterogeneous populations.
A characteristic clinical feature of COVID-19 is the frequent incidence microvascular thrombosis. In fact, autopsy reports have shown widespread thrombotic microangiopathy characterized by extensive diffuse microthrombi within peripheral capillaries and arterioles in lungs, hearts, other organs, resulting multiorgan failure. However, underlying process COVID-19-associated thrombosis remains elusive due to lack tools statistically examine platelet aggregation (i.e., initiation microthrombus...
Machine-learning-assisted identification of platelet aggregation in human blood with label-free images.
Imaging flow cytometry (IFC) has become a powerful tool for diverse biomedical applications by virtue of its ability to image single cells in high-throughput manner. However, there remains challenge posed the fundamental trade-off between throughput, sensitivity, and spatial resolution. Here we present deep-learning-enhanced imaging (dIFC) that circumvents this implementing an restoration algorithm on virtual-freezing fluorescence (VIFFI) platform, enabling higher throughput without...
view Abstract Citations (37) References (25) Co-Reads Similar Papers Volume Content Graphics Metrics Export Citation NASA/ADS Hard X-ray imaging observations of solar hot thermal flares with the HINOTORI spacecraft Tsuneta, S. ; Nitta, N. Takakura, T. Makishima, K. Murakami, Oda, M. Ogawara, Y. Ohki, Tanaka, Two hard bursts a new type (hot flare) were observed telescopes and other instruments on Japanese Hinotori. The have no clear impulsive phase below 40 keV emit intense X-rays (10-50 keV)...
Abstract Although CD4+CD25+ regulatory T (Treg) cells are known to suppress Th1 cell-mediated immune responses, their effect on Th2-type responses remains unclear. In this study we examined the role of Treg in airway inflammation mice. Depletion and reconstitution experiments demonstrated that naive mice effectively suppressed initiation development Th2-driven inflammation. Despite effective suppression mice, adoptively transferred, allergen-specific were unable allergen-presensitized...
Microalgal biofuels and biomass have ecofriendly advantages as feedstocks. Improved understanding utilization of microalgae require large-scale analysis the morphological metabolic heterogeneity within populations. Here, with Euglena gracilis a model microalgal species, we evaluate how fluorescence- brightfield-derived-image-based descriptors vary during environmental stress at single-cell level. This is achieved new multiparameter fluorescence-imaging cytometric technique that allows...
Introduction: Cyt-Geist: Current and Future Challenges in Cytometry 601 Chapter 01: Trends 01 Introduction WS18: Flow Drivers WS06: the Era of Human Cell Atlas 000 WS07: CYTO Lab Hacks: Inspiring Innovation Through Open Collaboration 605 WS15: The Status Microbiome 607 Conclusion 610 02: Shared Resource Laboratory (SRL) Best Practices 02 WS01: Balancing Science Service a 611 WS02: A Successful Imaging Facility: Guidelines for Management Publication 613 WS05: Identification Areas Where...
Abstract There is a global concern about the safety of COVID‐19 vaccines associated with platelet function. However, their long‐term effects on overall activity remain poorly understood. Here we address this problem by image‐based single‐cell profiling and temporal monitoring circulating aggregates in blood healthy human subjects, before after they received multiple Pfizer‐BioNTech (BNT162b2) vaccine doses over time span nearly 1 year. Results show no significant or persisting aggregation...
Abstract Intelligent image‐activated cell sorting (iIACS) has enabled high‐throughput image‐based of single cells with artificial intelligence (AI) algorithms. This AI‐on‐a‐chip technology combines fluorescence microscopy, AI‐based image processing, sort‐timing prediction, and sorting. Sort‐timing prediction is particularly essential due to the latency on order milliseconds between acquisition sort actuation, during which processing performed. The long amplifies effects fluctuations in flow...