A5069143697- Microbial Community Ecology and Physiology
- Wastewater Treatment and Nitrogen Removal
- Gut microbiota and health
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Microbial Fuel Cells and Bioremediation
- Single-cell and spatial transcriptomics
- Bacterial Genetics and Biotechnology
- Cell Image Analysis Techniques
- Fungal and yeast genetics research
- Protist diversity and phylogeny
- Gene Regulatory Network Analysis
- Marine and coastal ecosystems
- Arctic and Antarctic ice dynamics
- Methane Hydrates and Related Phenomena
- Bacterial biofilms and quorum sensing
- Environmental DNA in Biodiversity Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Constructed Wetlands for Wastewater Treatment
- Biosensors and Analytical Detection
- Advanced Proteomics Techniques and Applications
- Phosphorus and nutrient management
- Water Treatment and Disinfection
- Fermentation and Sensory Analysis
- Microbial bioremediation and biosurfactants
Helmholtz Centre for Environmental Research
2016-2025
Universität Greifswald
2019
University of Helsinki
2011-2016
Zero to Three
2016
ETH Zurich
2015
Deutsches Biomasseforschungszentrum
2001-2013
Medizinische Hochschule Hannover
2011
GEOMAR Technologie GmbH - GTG
1997-2000
Leipzig University
1992-2000
Saxon Institute for Applied Biotechnology
1992-1999
Viability measurements of individual bacteria are applied in various scopes research and industry using approaches where propidium iodide (PI) serves as dead cell indicator. The reliability PI uptake a viability indicator for (PI permeable) viable impermeable) was tested two soil bacteria, the gram(-) Sphingomonas sp. LB126 gram(+) Mycobacterium frederiksbergense LB501T.Bacterial proliferation activities observed viaDAPI Hoechst 33342 staining were linked to energy charge proportion cells...
Plasmids are widely used for molecular cloning or production of proteins in laboratory and industrial settings. Constant modification has brought forth countless plasmid vectors whose characteristics terms average copy number (PCN) stability rarely known. The crucial factor determining the PCN is replication system; most systems use today belong to a small different classes available through repositories like Standard European Vector Architecture (SEVA). In this study, was determined set...
The diversity and dynamics of a bacterial community extracted from an exploited oil field with high natural soil salinity near Comodoro Rivadavia in Patagonia (Argentina) were investigated. Community shifts during long-term incubation diesel fuel at four salinities between 0 20% NaCl monitored by single-strand conformation polymorphism fingerprinting the PCR-amplified V4-V5 region 16S rRNA genes. Information obtained this qualitative approach was extended flow cytometric analysis to follow...
ABSTRACT Wastewater treatment plants with enhanced biological phosphorus removal represent a state-of-the-art technology. Nevertheless, the process of phosphate is prone to occasional failure. One reason lack knowledge about structure and function bacterial communities involved. Most bacteria are still not cultivable, their functions during wastewater therefore unknown or subject speculation. Here, flow cytometry was used identify capable polyphosphate accumulation within highly diverse...
Populations of genetically identical microorganisms residing in the same environment can display marked variability their phenotypic traits; this phenomenon is termed heterogeneity. The relevance such heterogeneity natural habitats unknown, because characterization a sufficient number single cells species complex microbial communities technically difficult. We report procedure that allows to measure bacterial populations from environments, and use it analyze N2 CO2 fixation green sulfur...
In this communication we demonstrate that electrochemically active microbial biofilms and their enrichment at the anode of a bioelectrochemical system (BES) can be quantitatively easily characterized by flow-cytometry. This analysis revealed anodic biofilm BES, formed from highly diverse community fed with single substrate artificial wastewater, was dominated only one phylotype.
Optical characteristics of individual bacterial cells natural communities can be measured with flow cytometry (FCM) in high throughput. The resulting data are visualized cytometric histograms. These histograms represent fingerprints microbial communities, e.g., at certain time points or microenvironmental conditions. Up to now four tools for analyzing the variation these available but have not yet been systematically compared regarding application: Dalmatian Plot, Cytometric Histogram Image...
The carboxylate platform is a promising technology for substituting petrochemicals in the provision of specific chemicals and liquid fuels. It includes chain elongation process that exploits reverse β-oxidation to elongate short-chain fatty acids forms more valuable medium-chain variants. pH value influences this through multiple mechanisms central effective product formation. Its influence on microbiome dynamics was investigated during anaerobic fermentation maize silage by combining flow...
This work aims to investigate the long-term behavior of interactions electrochemically active bacteria in bioelectrochemical systems. The electrochemical performance and biofilm characteristics pure cultures Geobacter sulfurreducens Shewanella oneidensis are being compared a defined mixed culture both organisms. While S. did not form cohesive stable biofilms on graphite anodes only yielded 0.034 ± 0.011 mA/cm2 as maximum current density by feeding each 5 mM lactate acetate, G. formed 69 μm...
Abstract Altering environmental conditions change structures of microbial communities. These effects have an impact on the single‐cell level and can be sensitively detected using community flow cytometry. However, although highly accurate, monitoring campaigns are still rarely performed applying this technique. One reason is limited access to pattern analysis approaches for evaluation cytometric data. In article, a new analyzing tool, Cytometric Histogram Image Comparison (CHIC), presented,...
Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast inexpensive tool to interrogate on single-cell level.
The widely established production of CH4 from renewable biomass in industrial scale anaerobic reactors may play a major role the future energy supply. It relies on methanogenic archaea as key organisms which represent bottleneck process. quantitative analysis these can help to maximize process performance, uncover disturbances before failure, and ultimately lead community-based control schemes. Existing qPCR fluorescence microscopy-based methods are very attractive but be cost-intensive...
Natural microbial communities affect human life in countless ways, ranging from global biogeochemical cycles to the treatment of wastewater and health via microbiome. In order probe, monitor, eventually control these communities, fast detection evaluation methods are required. facilitate rapid community analysis monitor a community's dynamic behavior with high resolution, we here apply flow cytometry, which provides single-cell-based high-dimensional data characterizing acuity over time. To...
In completely insular microbial communities, evolution of community structure cannot be shaped by the immigration new members. addition, when those communities are run in steady state, influence environmental factors on their assembly is reduced. Therefore, one would expect similar structures under steady-state conditions. Yet, parallel setups, variability does occur. To reveal ecological mechanisms behind this phenomenon, five reactors were studied at single-cell level for about 100...
Many functions in host–microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on composition and functionality gut microbiota. To analyze these effects, we cultivated model community SIHUMIx bioreactors order to determine varying (TT) structure function. After five days continuous cultivation, investigated influence different medium TT 12 h, 24 48 h. For profiling microbial community, applied flow...