A5011644561- Innovative Microfluidic and Catalytic Techniques Innovation
- Viral Infectious Diseases and Gene Expression in Insects
- 3D Printing in Biomedical Research
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Microfluidic and Capillary Electrophoresis Applications
- Biofuel production and bioconversion
- Microfluidic and Bio-sensing Technologies
- Fluid Dynamics and Mixing
- Enzyme-mediated dye degradation
- Protein purification and stability
- Fungal Biology and Applications
- Enzyme Catalysis and Immobilization
- Bacterial biofilms and quorum sensing
- Microbial Fuel Cells and Bioremediation
- Fungal and yeast genetics research
- Biopolymer Synthesis and Applications
- Algal biology and biofuel production
- Bacteriophages and microbial interactions
- Plant tissue culture and regeneration
- Microbial Natural Products and Biosynthesis
- Plant Pathogens and Fungal Diseases
- Minerals Flotation and Separation Techniques
- Proteins in Food Systems
- Microbial Metabolism and Applications
Technische Universität Braunschweig
2016-2025
Jagiellonian University
2020
John Wiley & Sons (United States)
2018
Hudson Institute
2018
Biochemical Society
2012
Institut für Klinische Forschung und Entwicklung
1994
Paderborn University
1991-1992
Abstract The present study describes the design of bio‐pellet morphologies industrial working horse Aspergillus niger strains in submerged culture. novel approach recruits intended addition titanate microparticles (TiSiO 4 , 8 µm) to growth medium. As tested for two recombinant producing fructofuranosidase and glucoamylase, enzyme titer by titanate‐enhanced cultures shake flasks was increased 3.7‐fold 150 U/mL (for fructofuranosidase) 9.5‐fold 190 glucoamylase) as compared control. This...
The filamentous fungus Aspergillus niger is a widely used strain in broad range of industrial processes from food to pharmaceutical industry. One the most intriguing and often uncontrollable characteristics this organism its complex morphology, ranging dense spherical pellets viscous mycelia depending on culture conditions. Optimal productivity correlates strongly with specific morphological form, thus making high demands process control.In about 50 2L stirred tank cultivations influence...
The improvement of anaerobic digestion was investigated in an interdisciplinary research group. Using four different methods mechanical cell disintegration the influence degree and parameters on performance process investigated. Analytical to describe cell-disruption had be developed. best results were obtained using a stirred ball mill high-pressure homogenizer. As result degradation is accelerated time can reduced, especially when immobilized micro-organisms. treatment digested sludge by...
This work aims to investigate the long-term behavior of interactions electrochemically active bacteria in bioelectrochemical systems. The electrochemical performance and biofilm characteristics pure cultures Geobacter sulfurreducens Shewanella oneidensis are being compared a defined mixed culture both organisms. While S. did not form cohesive stable biofilms on graphite anodes only yielded 0.034 ± 0.011 mA/cm2 as maximum current density by feeding each 5 mM lactate acetate, G. formed 69 μm...
Abstract Filamentous fungal cell factories are efficient producers of platform chemicals, proteins, enzymes and natural products. Stirred-tank bioreactors up to a scale several hundred m³ commonly used for their cultivation. Fungal hyphae self-assemble into various cellular macromorphologies ranging from dispersed mycelia, loose clumps, compact pellets. Development these is so far unpredictable but strongly impacts productivities bioprocesses. Depending on the strain desired product,...
Trichoderma atroviride IMI 206040 synthesizes the coconut lactone 6-pentyl-α-pyrone (6-PAP) de novo and Aspergillus niger DSM 821 produces rose-like flavour compound 2-phenylethanol (2-PE) from precursor l-phenylalanine. Here, microparticles of different chemical composition nominal particle diameter in range 5–250 µm were added to shake-flask cultures both fungi investigate particles' effect on product formation. Maximum 2-PE concentration increased by a factor 1.3 1430 mg/l with addition...
• Two recombinant beta-lactoglobulin (BLG) B variants were produced in E. coli . Production/isolation resulted native BLG without post-translational modifications. Properties of compared to commercial and AB. Minor differences solubility interface adsorption behavior evident. Genetically engineered natural BLGs show similar emulsifying properties.
Precision fermentation is a promising route to develop recombinant proteins with improved functionality. Protein functionality highly influenced by protein structure, which can be significantly affected conformational restraints induced cysteines. Here, we study the impact of individual cysteines on β-lactoglobulin stepwise substituting them alanine. Structural characterisation was performed from secondary quaternary level. The findings demonstrated that free Cys121 played no role in folding...
Abstract Morphology has a crucial effect on productivity and the supply of substrate for cultures filamentous fungi. However, cultivation parameters leading to desired morphology are often chosen empirically as mechanisms governing processes involved usually unknown. For coagulating microorganisms like Aspergillus niger morphological development is considered start with aggregation conidia right after inoculation. To elucidate mechanism this process, kinetic studies were carried out using an...
In this work, a three-dimensional model of fluid-structure interactions (FSI) in biofilm systems is developed order to simulate detachment as result mechanical processes. Therein, fluid flow past the surface results load on structure which turn causes internal stresses matrix. When strength matrix exceeded parts are detached. The used investigate influence several parameters related matrix, Young's modulus, Reynolds number, and detachment. Variations conditions significantly simulation...
In vitro studies of ocular bioavailability active pharmaceutical ingredients (API) from colloidal drug delivery systems do not consider physiological shear stress generated by eyelid wiping and tear flow. The present study introduces a live cell imaging approach which enables the investigation model uptake various formulations under using custom-made microchannels for cultivation human corneal epithelial cells (HCE-T). Coumarin-6 (C-6) was used as API incorporated into solid lipid...
Abstract Many industrial biotechnological processes use filamentous microorganisms to produce platform chemicals, proteins, enzymes and natural products. Product formation is directly linked their cellular morphology ranging from dispersed mycelia over loose clumps compact pellets. Therefore, the adjustment control of pose major challenges for bioprocess engineering. Depending on strain desired product, optimal morphological shapes achieving high product concentrations vary. However, there...
Functional nanofibrils from globular proteins are usually formed by heating for several hours at pH 2.0, which induces acidic hydrolysis and consecutive self-association. The functional properties of these micro-metre-long anisotropic structures promising biodegradable biomaterials food applications, but their stability > 2.0 is low. results presented here show that modified β-lactoglobulin can also form neutral without prior hydrolysis; the key removing covalent disulfide bonds via...