A5020191368- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Innovative Microfluidic and Catalytic Techniques Innovation
- Single-cell and spatial transcriptomics
- Algal biology and biofuel production
- Fermentation and Sensory Analysis
- 3D Printing in Biomedical Research
- Biofuel production and bioconversion
- Molecular Biology Techniques and Applications
- Fungal Biology and Applications
- Mineral Processing and Grinding
- RNA Research and Splicing
- Plant Pathogens and Fungal Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Fluid Dynamics and Mixing
- biodegradable polymer synthesis and properties
- Chromatography in Natural Products
- Microfluidic and Capillary Electrophoresis Applications
- Enzyme Structure and Function
- Studies on Chitinases and Chitosanases
- Genomics and Phylogenetic Studies
- Slime Mold and Myxomycetes Research
- Lichen and fungal ecology
- Bioinformatics and Genomic Networks
- Biocrusts and Microbial Ecology
Technical University of Munich
2011-2024
Cell Biotech (South Korea)
2023
Biocat
2023
MS-Schramberg (Germany)
1982
University of Hohenheim
1969
Abstract Filamentous fungal cell factories are efficient producers of platform chemicals, proteins, enzymes and natural products. Stirred-tank bioreactors up to a scale several hundred m³ commonly used for their cultivation. Fungal hyphae self-assemble into various cellular macromorphologies ranging from dispersed mycelia, loose clumps, compact pellets. Development these is so far unpredictable but strongly impacts productivities bioprocesses. Depending on the strain desired product,...
Filamentous fungi are exploited as cell factories in biotechnology for the production of proteins, organic acids, and natural products. Hereby, fungal macromorphologies adopted during submerged cultivations bioreactors strongly impact productivity. In particular, pellets known to limit diffusivity oxygen, substrates, To investigate spatial distribution substances inside pellets, diffusive mass transport must be locally resolved. this study, we present a new approach obtain effective pellet...
To achieve climate neutrality, fundamentally new concepts of circularity need to be implemented by the building sector as it contributes 40% anthropogenic CO2 emission. Fungal biotechnology can make a significant contribution here and help eliminate fossil dependency for material production. Recently, we have shown that medicinal polypore Fomes fomentarius feeds well on renewable lignocellulosic biomass produces composite materials could potentially replace fuel-based expanded polystyrene...
In quantitative single-cell studies, the critical part is low amount of nucleic acids present and resulting experimental variations. addition biological data obtained from heterogeneous tissue are not reflecting expression behaviour every single-cell. These variations can be derived natural variance or introduced externally. Both have negative effects on quantification result. The aim this study to make studies more transparent reliable in order fulfil MIQE guidelines at level. technical...
Filamentous fungal cell factories play a pivotal role in biotechnology and circular economy. Hyphal growth macroscopic morphology are critical for product titers; however, these difficult to control predict. Usually pellets, which dense networks of branched hyphae, formed during industrial cultivations. They nutrient- oxygen-depleted their core due limited diffusive mass transport, compromises productivity bioprocesses. Here, we demonstrate that generalized law transport exists filamentous...
Filamentous fungi produce a wide range of relevant biotechnological compounds. The close relationship between fungal morphology and productivity has led to variety analytical methods quantify their macromorphology. Nevertheless, only µ-computed tomography (µ-CT) based method allows detailed analysis the 3D micromorphology pellets. However, low sample throughput laboratory µ-CT limits tracking micromorphological evolution statistically representative number submerged cultivated pellets over...
Members of the fungal kingdom are heterotrophic eukaryotes encased in a chitin containing cell wall. This polymer is vital for wall stiffness and, ultimately, shape. Most genomes contain numerous putative synthase encoding genes. However, systematic functional analysis full catalogue given species rare. greatly limits fundamental understanding and potential applications manipulating synthesis across kingdom. In this study, we conducted silico profiling subsequently deleted all predicted...
In quantitative single-cell studies, the critical part is low amount of nucleic acids present and resulting experimental variations. addition biological data obtained from heterogeneous tissue are not reflecting expression behaviour every single-cell. These variations can be derived natural variance or introduced externally. Both have negative effects on quantification result. The aim this study to make studies more transparent reliable in order fulfil MIQE guidelines at level. technical...
Abstract Many filamentous fungi are exploited as cell factories in biotechnology. Cultivated under industrially relevant submerged conditions, can adopt different macromorphologies ranging from dispersed mycelia over loose clumps to pellets. Central the development of a pellet morphology is agglomeration spores after inoculation followed by spore germination and outgrowth into population, which usually very heterogeneous. As dynamics underlying population heterogeneity not yet fully...
Abstract Controlling the morphology of filamentous fungi is crucial to improve performance fungal bioprocesses. Microparticle‐enhanced cultivation (MPEC) increases productivity, most likely by changing morphology. However, due a lack appropriate methods, exact impact added microparticles on structural development pellets mostly unexplored. In this study synchrotron radiation‐based microcomputed tomography and three‐dimensional (3D) image analysis were applied unveil detailed 3D incorporation...
ABSTRACT Filamentous fungi cultivated as biopellets are well established in biotechnology industries. A distinctive feature of filamentous is that hyphal growth and fungal morphology affect product titers require tailored process conditions. Within the pellet, mass transfer, substrate consumption, biomass formation intricately linked to local fraction pellet size. This study combined oxygen concentration measurements with microelectrode profiling three‐dimensional X‐ray microtomography same...