Biogas production from lignocellulosic feedstock not competing with food can contribute to a sustainable bioenergy system. The hydrolysis is the rate-limiting step in anaerobic digestion of solid substrates such as straw. Hence, detailed understanding metabolic processes during steps and acidogenesis required improve process control strategies. fermentation products formed acidogenic maize silage model substrate leach-bed were determined by gas liquid chromatography. bacterial community...

The persistence of acesulfame (ACE) in wastewater treatment (and subsequently the aquatic environment) has led to its use as a marker substance for input into surface water and groundwater. However, ACE degradation >85% during summer autumn was observed nine German plants (WWTPs). Annual removal performance more stable larger plants, enhanced by low biological oxygen demand impeded temperatures below 10 °C. Literature data suggest that potential degrade emerged WWTPs around year 2010. This...

Cattle manure is frequently used as an inoculum for the start-up of agricultural biogas plants or a co-substrate in anaerobic digestion lignocellulosic feedstock. Ruminal microbiota are considered to be effective plant fiber degraders, but microbes contained do not necessarily reflect rumen microbiome. The aim this study was compare microbial community composition cow and with respect fiber-digesting microbes. Bacterial methanogenic communities samples were examined by 454 amplicon...

The diversity and dynamics of a bacterial community extracted from an exploited oil field with high natural soil salinity near Comodoro Rivadavia in Patagonia (Argentina) were investigated. Community shifts during long-term incubation diesel fuel at four salinities between 0 20% NaCl monitored by single-strand conformation polymorphism fingerprinting the PCR-amplified V4-V5 region 16S rRNA genes. Information obtained this qualitative approach was extended flow cytometric analysis to follow...

ABSTRACT Wastewater treatment plants with enhanced biological phosphorus removal represent a state-of-the-art technology. Nevertheless, the process of phosphate is prone to occasional failure. One reason lack knowledge about structure and function bacterial communities involved. Most bacteria are still not cultivable, their functions during wastewater therefore unknown or subject speculation. Here, flow cytometry was used identify capable polyphosphate accumulation within highly diverse...

The microbial communities of in situ reactor columns degrading benzene with sulfate as an electron acceptor were analyzed based on clone libraries and terminal restriction fragment length polymorphism fingerprinting PCR-amplified 16S rRNA genes. filled either lava granules or sand particles percolated groundwater from a benzene-contaminated aquifer. predominant organisms colonizing the related to Magnetobacterium sp., followed by phylotype affiliated genera Cryptanaerobacter/Pelotomaculum...

Summary The flow of carbon under sulfate‐reducing conditions within a benzene‐mineralizing enrichment culture was analysed using fully labelled [ 13 C 6 ]‐benzene. Over 180 days incubation, 95% added C‐benzene released as C‐carbon dioxide. DNA extracted from cultures that had degraded different amounts unlabelled or C‐labelled benzene centrifuged in CsCl density gradients to identify C‐benzene‐assimilating organisms by density‐resolved terminal restriction fragment length polymorphism...

Biogas is an important renewable energy carrier. It a product of stepwise anaerobic degradation organic materials by highly diverse microbial communities forming complex interlinking metabolic networks. Knowledge about the background long-term stable process performance in full-scale reactors crucial for rationally improving efficiency and reliability biogas plants. To generate such knowledge, present study three parallel mesophilic fed exclusively with crops were sampled weekly over one...

Trace elements play an essential role in all organisms due to their functions enzyme complexes. In anaerobic digesters, control and supplementation of trace lead stable more efficient methane production processes while element deficits cause process imbalances. However, the underlying metabolic mechanisms adaptation affected microbial communities such are not yet fully understood. Here, we investigated community dynamics resulting changes induced by deprivation. Two identical lab-scale...

Micro-organisms are known to degrade a wide range of toxic substances. How the environment shapes microbial communities in polluted ecosystems and thus influences degradation capabilities is not yet fully understood. In this study, we investigated highly complex environment: capillary fringe subjacent sediments hydrocarbon-contaminated aquifer. Sixty sediment sections were analysed using terminal restriction fragment length polymorphism (T-RFLP) fingerprinting, cloning sequencing bacterial...

The microbial oxidation of sulfide is a key reaction the sulfur cycle, recycling in its most reduced valence state back to more oxidized forms usable as electron acceptors. Under anoxic conditions, nitrate preferential acceptor for this process. Two enzymatic pathways have been proposed under reducing sulfide:quinone oxidoreductase (SQR) pathway and Sox (sulfur oxidation) system. In experiments with model strains Thiobacillus denitrificans Sulfurimonas denitrificans, both resulted similar...

The carboxylate platform is a promising technology for substituting petrochemicals in the provision of specific chemicals and liquid fuels. It includes chain elongation process that exploits reverse β-oxidation to elongate short-chain fatty acids forms more valuable medium-chain variants. pH value influences this through multiple mechanisms central effective product formation. Its influence on microbiome dynamics was investigated during anaerobic fermentation maize silage by combining flow...

Ammonia inhibition is an important reason for reactor failures and economic losses in anaerobic digestion. Its impact on acetic acid degradation well studied, while its effect propionic butyric has received little attention consequently not considered the Anaerobic Digestion Model No. 1 (ADM1). To compare ammonia of these three volatile fatty acids (VFAs), we fed a mixture them as sole carbon source to continuous stirred tank reactors increased ammonium bicarbonate concentrations influent...

Anaerobic digestion is a complex multi-stage process relying on the activity of highly diverse microbial communities including hydrolytic, acidogenic and syntrophic acetogenic bacteria as well methanogenic archaea. The lower diversity archaea compared to bacterial groups involved in anaerobic corresponding lack functional redundancy cause stronger susceptibility methanogenesis unfavorable conditions such trace element deprivation, thus controlling stability overall process. Here, we...

Medium-chain carboxylates such as n-caproate and n-caprylate are valuable chemicals, which can be produced from renewable feedstock by anaerobic fermentation lactate-based microbial chain elongation. Acidogenic microbiota involved in elongation their interplay with lactic acid bacteria have not been characterized detail yet. Here, the metabolic community dynamics were studied a continuous bioreactor xylan lactate sole carbon sources. Four succession stages observed during 148 days of...

Biomethanation is a promising solution to convert H2 (produced from surplus electricity) and CO2 CH4 by using hydrogenotrophic methanogens. In ex situ biomethanation with mixed cultures, homoacetogens methanogens compete for H2/CO2. We enriched microbiota on as sole carbon energy sources, respectively, investigate these competing reactions. The microbial community structure dynamics of bacteria methanogenic archaea were evaluated through 16S rRNA mcrA gene amplicon sequencing, respectively....

Electron donor scarcity is seen as one of the major issues limiting economic production medium-chain carboxylates from waste streams. Previous studies suggest that co-fermentation hydrogen in microbial communities realize chain elongation relieves this limitation. To better understand how co-feeding can support elongation, we enriched three different anaerobic reactors (A, B, and C with ascending levels diversity) for their ability to produce conventional electron donors (lactate or ethanol)...