A5068106192- Microbial Community Ecology and Physiology
- Microbial bioremediation and biosurfactants
- Methane Hydrates and Related Phenomena
- Wastewater Treatment and Nitrogen Removal
- CO2 Sequestration and Geologic Interactions
- Atmospheric and Environmental Gas Dynamics
- Microbial Fuel Cells and Bioremediation
- Hydrocarbon exploration and reservoir analysis
- Groundwater and Isotope Geochemistry
- Mass Spectrometry Techniques and Applications
- Toxic Organic Pollutants Impact
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Proteomics Techniques and Applications
- Genomics and Phylogenetic Studies
- Groundwater flow and contamination studies
- Anaerobic Digestion and Biogas Production
- Water Treatment and Disinfection
- Isotope Analysis in Ecology
- Metabolomics and Mass Spectrometry Studies
- Mine drainage and remediation techniques
- Microbial Metabolic Engineering and Bioproduction
- Constructed Wetlands for Wastewater Treatment
- Metal Extraction and Bioleaching
- Gut microbiota and health
- Radioactive contamination and transfer
Helmholtz Centre for Environmental Research
2016-2025
Leipzig University
1994-2010
Horia Hulubei National Institute for R and D in Physics and Nuclear Engineering
2009
University of Bucharest
2009
Deutsches Biomasseforschungszentrum
2002-2004
Centre for European Economic Research
2003
Leibniz Institute for Solid State and Materials Research
2000-2002
Leibniz Institute for Neurobiology
2001-2002
Czech Academy of Sciences, Institute of Analytical Chemistry
1997-1999
University of Bremen
1996-1998
Recently, combined carbon and hydrogen isotope fractionation investigations have emerged as a powerful tool for the characterization of reaction mechanisms relevant removal organic pollutants. Here, we applied this approach in order to differentiate benzene biodegradation pathways under oxic anoxic conditions laboratory experiments. Carbon was studied with four different aerobic strains using monooxygenase or dioxygenase initial attack, facultative anaerobic chlorate-reducing strain well...
Polycyclic aromatic hydrocarbons (PAH) are widespread and persistent environmental contaminants, especially in oxygen-free environments. The occurrence of anaerobic PAH-degrading bacteria their underlying metabolic pathways rarely known. In this study, PAH degraders were enriched laboratory microcosms under sulfate-reducing conditions using groundwater sediment samples from four PAH-contaminated aquifers. Five enrichment cultures obtained showing sulfate-dependent naphthalene degradation....
Toluene degradation by several pure and mixed microbial cultures was investigated two-dimensional compound specific isotope analysis (2D-CSIA). For most of the cultures, respective toluene pathway attacking enzymatic step known. The slope linear regression for hydrogen (Δδ2H) vs carbon (Δδ13C) discrimination (Λ = Δδ2H/Δδ13C ≈ εHbulk/εCbulk) determined in order to characterize aerobic anaerobic pathways. highest Λ value estimated monohydroxylation methyl group Pseudomonas putida 53 ± 5)....
The microbial communities of in situ reactor columns degrading benzene with sulfate as an electron acceptor were analyzed based on clone libraries and terminal restriction fragment length polymorphism fingerprinting PCR-amplified 16S rRNA genes. filled either lava granules or sand particles percolated groundwater from a benzene-contaminated aquifer. predominant organisms colonizing the related to Magnetobacterium sp., followed by phylotype affiliated genera Cryptanaerobacter/Pelotomaculum...
Summary The flow of carbon under sulfate‐reducing conditions within a benzene‐mineralizing enrichment culture was analysed using fully labelled [ 13 C 6 ]‐benzene. Over 180 days incubation, 95% added C‐benzene released as C‐carbon dioxide. DNA extracted from cultures that had degraded different amounts unlabelled or C‐labelled benzene centrifuged in CsCl density gradients to identify C‐benzene‐assimilating organisms by density‐resolved terminal restriction fragment length polymorphism...
Summary In anaerobic bacteria, most aromatic growth substrates are channelled into the benzoyl‐coenzyme A (CoA) degradation pathway where ring is dearomatized and cleaved an aliphatic thiol ester. The initial step of this catalysed by dearomatizing benzoyl‐CoA reductases yielding two electron‐reduction product, cyclohexa‐1,5‐diene‐1‐carbonyl‐CoA, to which water subsequently added a hydratase. next steps have so far only been studied in facultative anaerobes comprise oxidation...
The microbial oxidation of sulfide is a key reaction the sulfur cycle, recycling in its most reduced valence state back to more oxidized forms usable as electron acceptors. Under anoxic conditions, nitrate preferential acceptor for this process. Two enzymatic pathways have been proposed under reducing sulfide:quinone oxidoreductase (SQR) pathway and Sox (sulfur oxidation) system. In experiments with model strains Thiobacillus denitrificans Sulfurimonas denitrificans, both resulted similar...
Carbon isotope fractionation factors were determined with the dichloro elimination of γ-hexachlorocyclohexane (γ-HCH) by sulfate-reducing bacteria Desulfococcus multivorans DSM 2059 and Desulfovibrio gigas 1382. Both strains are known for cometabolic HCH dechlorination. Degradation experiments γ-HCH in concentrations 22−25 μM carried out using benzoate (for D. multivorans) lactate gigas) as electron donors, respectively. was dechlorinated both bacterial within four weeks, metabolites...