A5048610924- Pesticide and Herbicide Environmental Studies
- Microbial bioremediation and biosurfactants
- Radioactive element chemistry and processing
- Pharmaceutical and Antibiotic Environmental Impacts
- Analytical chemistry methods development
- Chemical Reactions and Isotopes
- Microbial Community Ecology and Physiology
- Toxic Organic Pollutants Impact
- Isotope Analysis in Ecology
- Environmental remediation with nanomaterials
- Weed Control and Herbicide Applications
- Water Treatment and Disinfection
- Minerals Flotation and Separation Techniques
- Radioactive contamination and transfer
- Environmental Toxicology and Ecotoxicology
- Microbial Fuel Cells and Bioremediation
- Mass Spectrometry Techniques and Applications
- Urbanism, Landscape, and Tourism Studies
- Wastewater Treatment and Nitrogen Removal
- Gene expression and cancer classification
- Protease and Inhibitor Mechanisms
- Heat shock proteins research
- Advanced Biosensing Techniques and Applications
- Chromium effects and bioremediation
- Porphyrin Metabolism and Disorders
University of Tübingen
2013-2025
LMU Klinikum
2009
Abstract Diethylenetriamine penta(methylenephosphonate) (DTPMP) and related aminopolyphosphonates (APPs) are widely used as chelating agents in household industrial applications. Recent studies have linked APP emissions to elevated levels of the herbicide glyphosate European surface waters. However, transformation processes products APPs environment largely unknown. We show that is formed from DTPMP by reaction with manganese at near neutral pH pure water wastewater. Dissolved Mn 2+ O 2 or...
Abstract Aminopolyphosphonates (APPs) are widely used as chelating agents, and their increasing release into the environment has raised concerns due to transformation aminomethylphosphonic acid (AMPA) glyphosate, compounds of controversial environmental impact. This highlights urgent need for detailed studies under controlled conditions. Despite availability various methods quantifying individual aminopolyphosphonates aminomonophosphonates, a green, low-cost approach simultaneous...
Chloroethenes like trichloroethene (TCE) are prevalent environmental contaminants, which may be degraded through reductive dechlorination. Chemical models such as cobalamine (vitamin B12) and its simplified analogue cobaloxime have served to mimic microbial To test whether in vitro vivo mechanisms agree, we combined carbon chlorine isotope measurements of TCE. Degradation-associated enrichment factors ε(carbon) ε(chlorine) (i.e., molecular-average effects) were -12.2‰ ± 0.5‰ -3.6‰ 0.1‰ with...
Here we describe a novel microarray platform that integrates all functions needed to perform any array-based experiment in compact instrument on the researcher's laboratory benchtop. Oligonucle otide probes are synthesized situ via light- activated process within channels of three-dimensional microfluidic reaction carrier. Arrays can be designed and produced hours according user's requirements. They processed fully automatic workflow. We have characterized this new with regard dynamic range,...
Aminopolyphosphonates (APPs) are strong chelating agents with growing use in industrial and household applications. In this study, we investigated the oxidation of bisphosphonate iminodi(methylene phosphonate) (IDMP) – a major transformation product (TP) numerous commercially used APPs potential precursor for aminomethylphosphonate (AMPA) on manganese dioxide (MnO2). Transformation batch experiments at pH 6 revealed AMPA phosphate as main TPs, phosphorus mass balance 80 to 92% throughout all...
Aquifer sediments, formed under varying depositional conditions, exhibit significant heterogeneity in their sedimentary architecture causing variability hydraulic and biogeochemical properties. The spatial arrangement of these properties controls the net turnover biogeochemically reactive environmentally relevant solutes floodplains. However, interlinkage between is still enigmatic. This study proposes using facies analyses to reconstruct paleoenvironmental conditions that control abundance...
Nitrate pollution of groundwater represents a critical environmental concern, with many aquifers exceeding ecological and health-related concentration limits. Microbial driven denitrification the primary mechanism for nitrate attenuation in aquifers. Despite robust understanding individual transformation steps, predicting turnover complex heterogeneous subsurface environment remains challenging. This is primarily due to uneven distribution potential electron donors (e.g., organic carbon)...
<title>Abstract</title> We demonstrate for the first time that broad-spectrum herbicide glyphosate is a stable transformation product during manganese-driven oxidation of diethylenetriamine penta(methylenephosphonate) (DTPMP), complexing agent widely used in household and industry applications. Glyphosate formation was observed at circumneutral pH (i) presence MnO<sub>2</sub> (with without dissolved O<sub>2</sub>) as well (ii) Mn<sup>2+</sup> O<sub>2</sub>. Maximum yields varied with...
Although arsenic (As) groundwater contamination in South and Southeast Asia is a threat to human health, mechanisms of its release from sediment are still not fully understood. In many aquifers, Fe(III) minerals often the main hosting phases for As their stability crucial mobility. Recently, new mechanism mobilization into was proposed with methane (CH4) serving as an electron donor microbially mediated reductive dissolution As-bearing minerals. To provide unequivocal evidence occurrence...
Mn(II)-catalyzed oxidation by molecular oxygen is considered a relevant process for the environmental fate of aminopolyphosphonate chelating agents such as aminotrismethylene phosphonate (ATMP). However, potential roles Mn(III)ATMP-species in underlying transformation mechanisms are not fully understood. We combined kinetic studies, compound-specific stable carbon isotope analysis, and equilibrium speciation modeling to shed light on significance Mn–ATMP species overall ATMP oxygen. The...
Application of compound-specific stable isotope approaches often involves comparisons enrichment factors (ε). Experimental determination ε-values is based on the Rayleigh equation, which relates change in measured ratios to decreasing substrate fractions and valid for closed systems. Even well-controlled batch experiments, however, this requirement not necessarily fulfilled, since repetitive sampling can remove a significant fraction analyte. For volatile compounds need appropriate...
Quantification of in situ (bio)degradation using compound-specific isotope analysis requires a known and constant enrichment factor (ε). Because reported factors for microbial dehalogenation chlorinated ethenes vary considerably we studied the potential effects metabolic adaptation to TCE respiration on fractionation (δ13C δ37Cl) model organism (Desulfitobacterium hafniesne Y51), which only has one reductive dehalogenase (PceA). Cells grown first time showed exponential growth until 109...
ABSTRACT The consequences of urbanisation for Earth's biogeochemical cycles are largely unexplored. Copper (Cu) in urban soils is being accumulated mainly due to anthropogenic activities under rapid urbanisation. increasing Cu concentrations may contribute altering soil nitrogen (N) cycling ecosystems through modulating denitrification processes. This research aims identify how impacts functions and denitrifier abundance. An park with a background total concentration 7.9μgg –1 was incubated...
Kinetic isotope effects have been used successfully to prove and characterize organic contaminant transformation on various scales including field laboratory studies. For tetrachloroethene (PCE) biotransformation, however, causes for the substantial variability of reported enrichment factors (ε) are still not deciphered (εC = -0.4 -19.0‰). Factors such as different reaction mechanisms masking fractionation by either limited intracellular mass transfer or rate-limitations within enzymatic...
Dispersive liquid–liquid microextraction (DLLME) is a promising approach to enrich (semi-)volatile organics from aqueous samples prior compound-specific stable isotope analysis (CSIA) by gas chromatography-isotope ratio mass spectrometry (GC-IRMS).
Febit AG develops an integrated benchtop instrument for in situ microarrays preparation, hybridization, readout and data analysis.
Humic substances (HSs) are important electron acceptors and donors in soils aquifers. The coupling of anoxic nitrogen (N) cycling to the function HSs as a redox battery, however, remains poorly understood. Mediated electrochemical analysis is an emerging tool determine properties (i.e., donating capacity (EDC), accepting (EAC), state) HS. However, presence nitrite (NO2–), central intermediate N-cycle, interferes with determination EAC. To eliminate this interference, we developed bioassay...
The variability of reported carbon and chlorine isotope enrichment factors (εC εCl) for trichloroethene (TCE) respiration even pure bacterial cultures limits the applicability compound-specific analysis (CSIA) quantitative assessment TCE biotransformation. As growth metabolism bacteria in environment can differ substantially from standardized laboratory conditions, we investigated effects environmentally relevant variables on microbial fractionation including adaptation period to exposure,...
Nitrate (NO3-) is one of the most serious contaminants in groundwater, frequently deteriorating water quality and groundwater use as drinking water. can, at specific environmental conditions, be removed within subsurface environment via natural biogeochemical processes, such denitrification dissimilatory nitrate reduction to ammonium (DNRA). Traditionally, research has concentrated on NO3- attenuation potential microbial activity largely overlooking an aquifers&#8217; sediment matrix a...
In a synthetic binary culture, cell numbers derived by qPCR did not reflect the contribution of individual strains to total substrate turnover.