A5086496689- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Microbial Community Ecology and Physiology
- Vibrio bacteria research studies
- Microbial bioremediation and biosurfactants
- Innovative Microfluidic and Catalytic Techniques Innovation
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Cancer Genomics and Diagnostics
- 3D Printing in Biomedical Research
- Water Treatment and Disinfection
- Artificial Intelligence in Healthcare and Education
- Microfluidic and Bio-sensing Technologies
- Environmental Chemistry and Analysis
- Pediatric health and respiratory diseases
- Fecal contamination and water quality
- Antimicrobial agents and applications
- Genomics and Phylogenetic Studies
- Lymphatic System and Diseases
- Microbial metabolism and enzyme function
- Advanced Biosensing Techniques and Applications
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Plant Pathogenic Bacteria Studies
National Institute of Public Health
2021
Akademia Łomżyńska
2021
Harvard University
2014
University of Lausanne
2008-2013
University of Essex
2009
University of Agriculture in Krakow
2006
Petroleum hydrocarbons are common contaminants in marine and freshwater aquatic habitats, often occurring as a result of oil spillage. Rapid reliable on-site tools for measuring the bioavailable hydrocarbon fractions, i.e., those that most likely to cause toxic effects or available biodegradation, would assist assessing potential ecological damage following progress cleanup operations. Here we examined suitability set different rapid bioassays (2-3 h) using bacteria expressing LuxAB...
Summary Genomic islands are DNA elements acquired by horizontal gene transfer that common to a large number of bacterial genomes, which can contribute specific adaptive functions, e.g. virulence, metabolic capacities or antibiotic resistances. Some genomic still self‐transferable and display an intricate life‐style, reminiscent both bacteriophages conjugative plasmids. Here we studied the dynamical process island excision intracellular reintegration using integrative element ICE clc from...
Significance Bacterial pathogens frequently use type 3 secretion systems (T3SS) to counteract host immune responses infection. T3SS expression is associated with increased virulence of Pseudomonas aeruginosa in humans and animal models, but T3SS-negative bacteria often are isolated from acutely chronically infected patients. We tested whether could “cheat” during mixed infections T3SS-positive a murine model acute pneumonia. cheating occurred the inflamed lung only when secreted...
Introduction: Cyt-Geist: Current and Future Challenges in Cytometry 601 Chapter 01: Trends 01 Introduction WS18: Flow Drivers WS06: the Era of Human Cell Atlas 000 WS07: CYTO Lab Hacks: Inspiring Innovation Through Open Collaboration 605 WS15: The Status Microbiome 607 Conclusion 610 02: Shared Resource Laboratory (SRL) Best Practices 02 WS01: Balancing Science Service a 611 WS02: A Successful Imaging Facility: Guidelines for Management Publication 613 WS05: Identification Areas Where...
A yeast isolate revealing unique enzymatic activities and substrate-dependent polymorphism was obtained from autochthonous microflora of soil heavily polluted with oily slurries. By means standard identification procedures the strain identified as Trichosporon cutaneum. Further molecular PCR product analyses ribosomal DNA confirmed identity genus Trichosporon. As it grew on methanol a sole carbon source, appeared to be methylotrophic. Furthermore, also able utilize formaldehyde....
Toxicity of chemical pollutants in aquatic environments is often addressed by assays that inquire reproductive inhibition test microorganisms, such as algae or bacteria. Those tests, however, assess growth populations a whole via macroscopic methods culture turbidity colony-forming units. Here we use flow cytometry to interrogate the fate individual cells low-density bacterium Pseudomonas fluorescens SV3 exposed not under oligotrophic conditions number common pollutants, some which derive...
Summary Biocides are widely used for domestic hygiene, agricultural and industrial applications. Their widespread use has resulted in their introduction into the environment raised concerns about potential deleterious effects on aquatic ecosystems. In this study, toxicity of biocides triclosan, penconazole metalaxyl were evaluated with freshwater bacterium Caulobacter crescentus a microbial community using combination single‐ double‐stain flow cytometric assays. Growth C. repressed by...
Pseudomonas azelaica HBP1 is one of the few bacteria known to completely mineralize biocide and toxic compound 2-hydroxybiphenyl (2-HBP), but mechanisms its tolerance toxicity are unknown. By transposon mutant analysis screening for absence growth on water saturating concentrations 2-HBP (2.7 mM) we preferentially found insertions in three genes with high homology mexA, mexB, oprM efflux system. Mutants could grow at below 100 μM lower rates than wild-type. Exposure wild-type increasing...
In May 2018, we initiated a new, unique project: the CYTO Conference workshop summary report. The main motivation was to present reader key findings from live discussions, indicate trends, and discuss identified challenges proposed solutions or guidelines in specific fields of cytometry. workshops are well-known dynamic open forums for free discussions experience exchange at different levels among various scientific specialties. allocated time this format however is limited, so must be run...
We show proof of principle for assessing compound biodegradation at 1-2 mg C per L by measuring microbial community growth over time with direct cell counting flow cytometry. The concept is based on the assumption that will increase in number through incorporation carbon from added test into new cells absence (as much as possible) other assimilable carbon. pure cultures bacterium Pseudomonas azelaica specific population can be measured low 0.1 2-hydroxybiphenyl L, whereas mixed 1 still...
Chemical pollution is known to affect microbial community composition but it poorly understood how toxic compounds influence physiology of single cells that may lay at the basis loss reproductive fitness. Here we analyze physiological disturbances a variety chemical pollutants cell level using bacterium Pseudomonas fluorescens in an oligotrophic growth assay. As proxy for disturbance measured changes geometric mean ethidium bromide (EB) fluorescence intensities subpopulations live and...
ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionORIGINAL ARTICLEThis notice is a correctionCorrection to A Flow Cytometry Based Oligotrophic Pollutant Exposure Test Detect Bacterial Growth Inhibition and Cell InjuryKamila Czechowska Jan Roelof van der MeerView Author Information Department of Fundamental Microbiology, University Lausanne, Bâtiment Biophore, Quartier UNIL-Sorge, 1015 SwitzerlandCite this: Environ. Sci. Technol. 2012, 46, 5, 3038Publication Date (Web):February 27,...
<p><strong>ABSTRACT</strong><br /><strong>INTRODUCTION.</strong> The National Health Fund guarantees to insured persons free dental treatment as part of a catalog scopes and benefits, such treatment. Among the services included in appendix there is plaque removal procedure, which can be performed adults once every 12 months or 6 case pregnant puerperal women. This procedure involves breaking down bacterial biofilm above below gingiva, rinsing it vases with...