A5035642184- Reproductive tract infections research
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Spaceflight effects on biology
- Chronic Myeloid Leukemia Treatments
- T-cell and B-cell Immunology
- Lipid Membrane Structure and Behavior
- Animal Virus Infections Studies
- Urinary Tract Infections Management
- Reproductive System and Pregnancy
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- Anesthesia and Neurotoxicity Research
- High Altitude and Hypoxia
- Herpesvirus Infections and Treatments
- Cell Image Analysis Techniques
- RNA Interference and Gene Delivery
- Syphilis Diagnosis and Treatment
- Legionella and Acanthamoeba research
- vaccines and immunoinformatics approaches
- Radiation Therapy and Dosimetry
- Ultrasound and Hyperthermia Applications
- Inflammasome and immune disorders
- Escherichia coli research studies
University of Zurich
2008-2020
Institute of Cell Biology and Neurobiology
2009
University Children's Hospital Zurich
2005
Abstract In our study we aimed to identify rapidly reacting gravity-responsive mechanisms in mammalian cells order understand if and how altered gravity is translated into a cellular response. combination of experiments using "functional weightlessness" provided by 2D-clinostats real microgravity several parabolic flight campaigns compared in-flight-1g-controls, identified rapid reactions inside the cell cycle regulatory machinery human T lymphocytes. response 2D clinorotation, detected an...
Cells of the immune system are highly sensitive to altered gravity, and monocyte as well macrophage function is proven be impaired under microgravity conditions. In our study, we investigated surface expression ICAM-1 protein mRNA in cells monocyte/macrophage during clinostat, parabolic flight, sounding rocket, orbital experiments. murine BV-2 microglial cells, detected a downregulation clinorotation experiments rapid reversible phase flight contrast, increased macrophage-like differentiated...
The effect of anesthetic drugs on cancer outcomes remains unclear. This trial aimed to assess postoperative circulating tumor cell counts-an independent prognostic factor for breast cancer-to determine how anesthesia may indirectly affect prognosis. It was hypothesized that patients receiving sevoflurane would have higher counts.The parallel, randomized controlled conducted in two centers Switzerland. Patients aged 18 85 yr without metastases and scheduled primary surgery were eligible....
Helicobacter pylori genomes typically contain 8 or 9 genes that code for secreted and highly disulfide‐bridged proteins designated cysteine‐rich (Hcp). Here we show HcpA (hp0211) but not HcpC (hp1098) triggers the differentiation of human myeloid Thp1 monocytes into macrophages. Small amounts cause transition round‐shaped cells with star‐like morphologies, adherence to culture dish surface, phagocytosis opsonized fluorescent microspheres, expression surface marker protein CD11b, all which...
During spaceflight the immune system is one of most affected systems human body. SIMBOX (Science in Microgravity Box) mission on Shenzhou-8, we investigated microgravity-associated long-term alterations macrophageal cells, important effector cells system. We analyzed effect microgravity cytoskeleton and immunologically relevant surface molecules. Human U937 were differentiated into a phenotype exposed to or 1g reference centrifuge on-orbit for 5 days. After fixation, samples with...
Monitoring of single cell signal transduction in leukemic cellular subsets has been proposed to provide deeper understanding disease biology and prognosis, but so far not tested a clinical trial targeted therapy. We developed complete mass cytometry analysis pipeline for characterization intracellular patterns the major leukocyte chronic phase myeloid leukemia. Changes phosphorylated Bcr-Abl1 signaling pathways involved were readily identifiable peripheral blood cells already within three...
Abstract Giant unilamellar vesicles (GUVs) are model cell-sized systems that have broad applications including drug delivery, analysis of membrane biophysics, and synthetic reconstitution cellular machineries. Although numerous methods for the generation free-floating GUVs been established over past few decades, only a fraction successfully produced uniform vesicle populations both from charged lipids in buffers physiological ionic strength. In method described here, we generate large...
Background After cerebral injury blood-brain barrier disruption significantly impairs brain homeostasis. Volatile anesthetics have been shown to be protective in ischemia-reperfusion scenarios. Their impact on endothelial cells after hypoxia-reoxygenation (H/R) has not yet studied detail. Methods Rat (RBE4) were exposed severe hypoxia and reoxygenated air the presence or absence of sevoflurane. Changes dextran permeability architecture cellular junctional proteins ZO-1 β-catenin measured. To...
Chlamydiae induce persistent infections, which have been associated with a wide range of chronic diseases in humans and animals. Mixed infections Chlamydia porcine epidemic diarrhea virus (PEDV) may result generation chlamydial infections. To test this hypothesis, an vitro model dual infection cell culture-adapted PEDV abortus or pecorum Vero cells was established. Infected cultures were investigated by immunofluorescence (IF), transmission electron microscopy (TEM) re-infection experiments....
Abstract The bacteriolytic activity of CTL is mediated by granulysin, which has been reported to kill intracellular Mycobacterium tuberculosis in dendritic cells (DC) with high efficiency. Despite that crucial effector function, the killing mechanism and uptake granulysin into target have not well investigated. To this end we analyzed binding, uptake, subsequent lysis Listeria innocua human DC. Recombinant was found be actively taken up DC early endosomal Ag 1-labeled endosomes, as detected...
Abstract Background The intracellular bacterium Chlamydia pneumoniae is suspected to play a role in formation and progression of atherosclerosis. Many studies investigated cell death initiation versus inhibition by established lines but nothing known primary human aortic smooth muscle cells, type among others be involved the atherosclerotic plaque. Type was analyzed various methods cells after infection with investigate possible pathogenic link Results Chlamydiae were found localized up 72 h...
Cytotoxic T lymphocytes (CTL) and natural killer (NK) cells play an essential role in the host defence against intracellular pathogens such as Listeria, Mycobacteria. The key mediator of bacteria-directed cytotoxicity is granulysin, a 9 kDa protein stored cytolytic granules together with perforin granzymes. Granulysin binds to cell membranes subsequently taken up via lipid raft-associated mechanism. In dendritic (DC) granulysin further transferred early endosomes L. innocua-containing...
Abstract Background Granulysin, a cytotoxic protein expressed in human natural killer cells and activated T lymphocytes, exhibits cytolytic activity against variety of intracellular microbes. Expression transcription have been partially characterised vitro four transcripts (NKG5, 519, 520, 522) were identified. However, only single product 15 kDa was found, which is subsequently processed to an active 9 protein. Results In this study we investigated generation granulysin lymphokine (LAK)...
Aims In order for Chlamydia pneumoniae to play a causative role in chronic human disease, it would need persist within infected tissue extended periods of time. Current theory suggests that C may at the site infection via an alternative replicative form, known as aberrant body. Methods A panel -specific antibodies upregulated by body was used probe specimens from coronary atheroma 13 explanted hearts identify patterns reactivity these tissues, well determine presence and prevalence bodies....
Aim: Magnetic field guided drug targeting holds promise for more effective cancer treatment. Intravascular application of magnetic nanoparticles, however, bears the risk potentially important, yet poorly understood side effects, such as off-target accumulation in endothelial cells. Materials & methods: Here, we investigated influence shear stress (0–3.22 dyn/cm2), exposure time (5–30 min) and activation on uptake ferromagnetic carbon-encapsulated iron carbide nanomagnets into cells an vitro...
In this study we investigated if and how cannabinoid receptor stimulation regulates macrophageal differentiation, which is one of the key steps in immune effector reaction. For that reason, used a well established differentiation model system human U937 myelocytic leukemia cells differentiate along monocyte/macrophage lineage upon with phorbol ester PMA. Constant (CB) was performed using WIN55212-2, potent synthetic CB agonist. We found WIN55212-2 inhibited CB1/2-receptor-dependent...
In May 2018, we initiated a new, unique project: the CYTO Conference workshop summary report. The main motivation was to present reader key findings from live discussions, indicate trends, and discuss identified challenges proposed solutions or guidelines in specific fields of cytometry. workshops are well-known dynamic open forums for free discussions experience exchange at different levels among various scientific specialties. allocated time this format however is limited, so must be run...
Abstract The development of preventive or therapeutic recombinant vaccines and the generation serodiagnostic assays for infectious diseases depend essentially on availability molecularly defined antigens. A major bottleneck identification suitable target antigens many pathogens is isolation sufficient amounts material subsequent genomic proteomic screening. Applying a highly efficient expression cloning strategy to human vaccinia virus (VV) Chlamydia pneumoniae (CP), we demonstrate that...
Abstract Background Atherosclerosis is still the leading cause of death in western world. Besides known risk factors studies demonstrating Chlamydophila pneumoniae ( C. ) to be implicated progression disease, little about infection dynamics. We investigated whether induce cell human aortic endothelial cells, a type involved initiation atherosclerosis, and chlamydial spots derive from inclusions. Results Lactate dehydrogenase release revealed host dependent on amounts Chlamydia used for...
Regulation of cytotoxic effector molecule expression in human CTLs after viral or bacterial activation is poorly understood. By using autologous dendritic cells (DCs) to prime T lymphocytes, we found perforin only highly up-regulated virus- (HSV-1, vaccinia virus) but not intracellular bacteria- (Listeria innocua, Listeria monocytogenes, Mycobacterium tuberculosis, Chlamydophila pneumoniae) activated CTLs. In contrast, larger quantities IFN-gamma and TNF-alpha were produced...
Viral infections during pregnancy significantly increase the risk for psychological pathologies like schizophrenia in offspring. One of main morphological hallmarks is a reduced size hippocampus. Since new neurons are produced this particular brain compartment throughout life, it might be possible that low neurogenesis levels triggered by maternal viral infection contribute to developmental deficits We injected polyinosinic:polycytidylic acid (Poly I:C) pregnant C57Bl/6 mice stimulate an...
Infection with the common pathogen Chlamydophila pneumoniae (Cpn, previously Chlamydia pneumoniae) has a high prevalence in patients suffering from arteriosclerosis and may trigger or contribute to heart disease. In mice, CD8-positive T cells are critical for eradication of infection development immune memory against Cpn. Although several H2-class I epitopes have been described, no HLA-class I-associated peptides Cpn known. order define HLA-A*0201 Cpn, we focused on bacterial heat shock...