A5090664312- Cancer, Hypoxia, and Metabolism
- DNA Repair Mechanisms
- Cancer, Lipids, and Metabolism
- Protease and Inhibitor Mechanisms
- Protein Hydrolysis and Bioactive Peptides
- melanin and skin pigmentation
- Polyamine Metabolism and Applications
- Cell Image Analysis Techniques
- Glutathione Transferases and Polymorphisms
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Silk-based biomaterials and applications
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Biomedical and Engineering Education
- Molecular Biology Techniques and Applications
- Antimicrobial Peptides and Activities
- Carcinogens and Genotoxicity Assessment
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Advanced Proteomics Techniques and Applications
- Histone Deacetylase Inhibitors Research
- Peptidase Inhibition and Analysis
- Biopolymer Synthesis and Applications
Wayne State University
2008-2024
The Barbara Ann Karmanos Cancer Institute
2008-2023
Juntendo University
2008
Subpopulations of cancer stem cells (CSCs) or stem-like (CSLCs) have been identified from most tumors, including pancreatic (PC), and the existence these is clinically relevant. Emerging evidence suggests that CSLCs participate in cell growth/proliferation, migration/invasion, metastasis, chemo-radiotherapy resistance, ultimately contributing to poor clinical outcome. However, pathogenesis biological significance PC has not well characterized. In present study, we found isolated...
One-carbon (C1) metabolism is compartmentalized between the cytosol and mitochondria with mitochondrial C1 pathway as major source of glycine units for cellular biosynthesis. Expression genes including SLC25A32, serine hydroxymethyl transferase (SHMT) 2, 5,10-methylene tetrahydrofolate dehydrogenase 1-like was significantly elevated in primary epithelial ovarian cancer (EOC) specimens compared normal ovaries. 5-Substituted pyrrolo[3,2-d]pyrimidine antifolates (AGF347, AGF359, AGF362)...
Abstract Future curative cancer chemotherapies have to overcome tumor cell heterogeneity and plasticity. To test the hypothesis that suppressor maspin may reduce microenvironment-dependent prostate plasticity thereby modulate drug sensitivity, we established a new schematic combination of two-dimensional (2D), three-dimensional (3D), suspension cultures enrich subpopulations with distinct differentiation potentials. We report here depending on level expression, cells in 3D collagen I...
Abstract Purpose: ERCC1/XPF is a DNA endonuclease with variable expression in primary tumor specimens, and has been investigated as predictive biomarker for efficacy of platinum-based chemotherapy. The failure clinical trials utilizing ERCC1 to predict response chemotherapy suggests additional mechanisms underlying the basic biology interstrand crosslinks (ICLs) remain unknown. We aimed characterize panel knockout (Δ) cell lines, where we identified synthetic viable phenotype ICLs...
Abstract Her-2/neu+ tumor cells refractory to antibody or receptor tyrosine kinase inhibitors are emerging in treated patients. To investigate if drug resistant tumors can be controlled by active vaccination, gefitinib and sensitivity of four neu+ BALB/c mouse mammary lines were compared. Significant differences cell proliferation Akt phosphorylation observed. Treatment-induced resistance was associated with increased chromosomal aberrations as shown spectral karyotyping analysis, suggesting...
// Sijana H. Dzinic 1,2 , Kang Chen 2,3,4,5,6,7 Archana Thakur 2,5 Alexander Kaplun 1,2,12 R. Daniel Bonfil 1,2,8 Xiaohua Li Jason Liu M. Margarida Bernardo Allen Saliganan 2,8 Jessica B. Back Hiroshi Yano Dana L. Schalk Elyse N. Tomaszewski Ahmed S. Beydoun Gregory Dyson Adelina Mujagic David Krass Ivory Dean Qing-Sheng Mi 2,4,9 Elisabeth Heath 8,11 Wael Sakr Lawrence G. Lum 2,4,5,10 and Shijie Sheng 1,2,5 1 Department of Pathology, Wayne State University School Medicine, Detroit, Michigan...
Abstract Novel therapies are urgently needed for epithelial ovarian cancer (EOC), the most lethal gynecologic malignancy. In addition, that target unique vulnerabilities in tumor microenvironment (TME) of EOC have largely been unrealized. One strategy to achieve selective drug delivery therapy involves use targeted antifolates via their uptake by folate receptor (FR) proteins, resulting inhibition essential one-carbon (C1) metabolic pathways. FRα is highly expressed EOCs, along with...
Abstract Dexamethasone (Dex), co-administered to lung adenocarcinoma patients with pemetrexed chemotherapy, protects against cytotoxicity by inducing reversible G1 arrest, reflected the effect of Dex on FLT-PET images patient tumors. However, perioperative treatment increases survival but mechanism is unknown. In cells glucocorticoid receptor-α (GR) expression corresponding higher clinical tumor levels, Dex-induced growth arrest was followed marked cell expansion, beta-galactosidase and Ki67...
Abstract ERCC1/XPF is a heterodimeric DNA endonuclease critical for repair of certain chemotherapeutic agents. We recently identified that ERCC1- and p53-deficient lung cancer cells are tolerant to platinum-based chemotherapy. ATR inhibition synergistically re-stored platinum sensitivity ERCC1-deficient cells. Mechanistically we show this effect reliant upon several functions including replication fork protection altered cell cycle checkpoints. Utilizing an inhibitor protein A (RPA), further...
In May 2018, we initiated a new, unique project: the CYTO Conference workshop summary report. The main motivation was to present reader key findings from live discussions, indicate trends, and discuss identified challenges proposed solutions or guidelines in specific fields of cytometry. workshops are well-known dynamic open forums for free discussions experience exchange at different levels among various scientific specialties. allocated time this format however is limited, so must be run...
Tissue-resident macrophages (ResMϕ) play important roles in the normal development and physiological functions as well tissue repair immune/inflammatory response to both internal external insults. In cornea, ResMϕ are critical homeostasis maintenance, wound healing, ocular immune privilege, injury microbial infection. However, of microRNAs corneal utterly unknown. Previously, we demonstrated that conserved miR-183/96/182 cluster (miR-183/96/182) plays sensory neurons subgroups innate...
Abstract A biosafety plan is essential to establish appropriate practices for in a shared resource laboratory (SRL). will contain the information use of biological samples on specific instrumentation, their apparent risks, and steps that should be taken mitigate these risks. Establishment can daunting task as variety pathogens come through SRL highly diverse may change over time; however, having adapt this provide framework addressing concerns educating personnel users practices. Using...
Abstract Undoubtedly, the global pandemic caused by SARS‐CoV‐2 virus has had a significant impact on Shared Resource Laboratories (SRL) operations worldwide. Unlike other crises (e.g., natural disasters, acts of war, or terrorism) which often result in sudden and sustained cessation scientific research usually affecting one two cities at time, this is being seen simultaneously every SRL worldwide albeit to varying degree. The alterations COVID‐19 can generally be divided into three...
<p>This table shows primer sequences for quantitative real-time RT-PCR.</p>
<div>Abstract<p>One-carbon (C1) metabolism is compartmentalized between the cytosol and mitochondria with mitochondrial C1 pathway as major source of glycine units for cellular biosynthesis. Expression genes including <i>SLC25A32</i>, <i>serine hydroxymethyl transferase (SHMT) 2</i>, <i>5,10-methylene tetrahydrofolate dehydrogenase 1-like</i> was significantly elevated in primary epithelial ovarian cancer (EOC) specimens compared normal...
<div>Abstract<p>One-carbon (C1) metabolism is compartmentalized between the cytosol and mitochondria with mitochondrial C1 pathway as major source of glycine units for cellular biosynthesis. Expression genes including <i>SLC25A32</i>, <i>serine hydroxymethyl transferase (SHMT) 2</i>, <i>5,10-methylene tetrahydrofolate dehydrogenase 1-like</i> was significantly elevated in primary epithelial ovarian cancer (EOC) specimens compared normal...
<p>This table shows information of epithelial ovarian cancer tissue microarray (TMA) for immunohistochemistry.</p>
<p>This figure shows immunohistochemical staining of tumor microarray (TMA) for SHMT2 protein.</p>
<p>This figure shows FRα, PCFT and RFC expression in EOC cell line models patient cDNAs.</p>
<p>This table shows IC50 values for antiproliferative activities toward EOC versus normal ovary cell lines by 5-substituted pyrrolo[3,2-d]pyrimidine inhibitors.</p>
<p>This table shows Kis for inhibition of one-carbon enzymes 5-substituted pyrrolo[3,2- d]pyrimidine antifolates.</p>
<p>This table shows primer sequences for quantitative real-time RT-PCR.</p>
<p>This figure shows purine nucleotides in SKOV3 EOC cells treated with pyrrolo[3,2-d]pyrimidine antifolates.</p>
<p>This table shows information of ovarian cancer tissue cDNA array for real-time RT-PCR.</p>