A5053675854- Immune Response and Inflammation
- Ocular Surface and Contact Lens
- Ocular Infections and Treatments
- Corneal Surgery and Treatments
- Antimicrobial Peptides and Activities
- Bacterial biofilms and quorum sensing
- Connexins and lens biology
- Ocular Diseases and Behçet’s Syndrome
- Pharmacological Effects of Natural Compounds
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Glaucoma and retinal disorders
- Immunotherapy and Immune Responses
- Advanced Glycation End Products research
- Corneal surgery and disorders
- Cell Adhesion Molecules Research
- Intraocular Surgery and Lenses
- Protease and Inhibitor Mechanisms
- Nanoplatforms for cancer theranostics
- Advanced Drug Delivery Systems
- Proteoglycans and glycosaminoglycans research
- Climate Change and Health Impacts
- Air Quality and Health Impacts
- Vibrio bacteria research studies
- Neuropeptides and Animal Physiology
- Antibiotic Resistance in Bacteria
Wayne State University
2015-2024
Michigan United
2015-2018
Kresge Eye Institute
2016
Institute of Cell Biology and Neurobiology
2000-2004
National Institutes of Health
2003
University of South Carolina
2003
World Vision
2003
University of Amsterdam
2003
Author(s): Ung, Lawson; Acharya, Nisha R; Agarwal, Tushar; Alfonso, Eduardo C; Bagga, Bhupesh; Bispo, Paulo Jm; Burton, Matthew J; Dart, John Kg; Doan, Thuy; Fleiszig, Suzanne Mj; Garg, Prashant; Gilmore, Michael S; Gritz, David Hazlett, Linda D; Iovieno, Alfonso; Jhanji, Vishal; Kempen, H; Lee, Cecilia Lietman, Thomas M; Margolis, Todd P; McLeod, Stephen Mehta, Jod Miller, Darlene; Pearlman, Eric; Prajna, Lalitha; N Venkatesh; Seitzman, Gerami Shanbhag, Swapna Sharma, Namrata; Savitri;...
Abstract The kinetics of IL-1 (α and β) production after Pseudomonas aeruginosa corneal infection was examined in susceptible (cornea perforates) C57BL/6J (B6) resistant heals) BALB/cByJ (BALB/c) mice. IL-1α -1β (mRNA protein) were elevated both mouse strains, levels peaked at 1 day postinfection (p.i.). Significantly greater amounts protein detected B6 vs BALB/c mice 3 days p.i. At 5 p.i., remained B6, but began to decline To test the significance mice, a polyclonal neutralizing Ab against...
Abstract Polymorphonuclear neutrophils (PMN) in Pseudomonas aeruginosa-infected cornea are required to clear bacteria from affected tissue, yet their persistence may contribute irreversible tissue destruction. This study examined the role of C-X-C chemokines PMN infiltration into P. and contribution these mediators disease pathology. After aeruginosa challenge, corneal number macrophage inflammatory protein-2 (MIP-2) KC levels were compared mice that susceptible (cornea perforates) or...
This study investigated which adhesins of Pseudomonas aeruginosa interact with the glycolipid asialo GM1, using solid-phase binding and thin-layer chromatography assays. Radioiodinated pili flagella contaminated lipopolysaccharide (LPS) bound to glycolipid. When LPS was reduced acceptable levels in pilus flagellum samples, only specifically Commercial, radiolabeled as well whole bacteria strain ATCC 19660 also GM1. Binding specific, competitive, saturable. Organ cultures mouse eyes scanning...
To determine the role of IL-33 in resistance to Pseudomonas aeruginosa keratitis.
ABSTRACT Using a multiprobe RNase protection assay, we examined cytokine and chemokine mRNAs that were expressed after corneal infection with Pseudomonas aeruginosa in mice. Cytokines upregulated included interleukin-1α (IL-1α) -1β, IL-1 receptor antagonist, IL-6, IL-11, granulocyte colony-stimulating factor, granulocyte-macrophage macrophage stem cell lymphotoxin β, transforming growth factor β1, tumor necrosis alpha. Chemokine transcripts Eotaxin; gamma-interferon-inducible protein 10;...
Abstract The role of T lymphocytes in susceptibility to Pseudomonas aeruginosa corneal infection was studied inbred C57Bl/6 (B6) beta2-microglobulin+/+ (beta2m+/+) and beta2m-/- knockout (KO) mice on a B6 genetic background. corneas both KO perforated by 7 days postinfection (p.i.). Histopathology revealed similar inflammatory response characterized an infiltration polymorphonuclear neutrophilic leukocytes 24 h p.i. groups mice. CD4+ CD8+ (latter absent KO) cells were present cornea 3 p.i.,...
To determine the effects of silencing Toll-like receptor (TLR) 9 signaling in Pseudomonas aeruginosa keratitis.Corneal TLR9 mRNA levels were tested by RT-PCR C57BL/6 (B6, susceptible) and BALB/c (resistant) mice compared. The response B6 to CpG DNA, which binds TLR9, was after subconjunctival injection with control or DNA; IL-1beta, macrophage inflammatory protein (MIP)-2, IL-4, IL-10, IL-12, IL-18, IFN-gamma measured RT-PCR. Langerhans cells (LCs) stimulated DNA treated siRNA, MIP-2...
purpose. To determine the role of Toll-like receptor 4 (TLR4) in Pseudomonas aeruginosa (P. aeruginosa) keratitis resistant (cornea-healing) BALB/c mice. methods. Corneal TLR4 mRNA levels were tested by real-time PCR mice before and after infection. Clinical score, slit lamp, histopathology, bacterial counts, polymorphonuclear neutrophil (PMN) quantitation performed infected cornea TLR4-deficient (TLR4lps-d) wild-type for IL-1β, MIP-2, IFN-γ, IL-18, inducible nitric oxide synthase (iNOS),...
ABSTRACT As a novel family of cell surface receptors, triggering receptors expressed on myeloid cells (TREMs) play an important role in inflammatory responses. However, the TREMs ocular immune system remains unknown. In this study, we examined expression and function TREM-1 Pseudomonas aeruginosa keratitis, one most common sight-threatening diseases. was significantly increased human corneas after P. infection. Consistent with at surface, levels (mRNA protein) were also elevated infected...
Hypoxia-inducible factor (HIF)-1α, is a transcription that controls energy metabolism and angiogenesis under hypoxic conditions, potent regulator of innate immunity. The studies described herein examined the role HIF-1α in disease resolution BALB/c (resistant, cornea heals) mice after ocular infection with Pseudomonas (P.) aeruginosa. Furthermore, current focused on neutrophil (PMN), predominant cell infiltrate keratitis. Using both siRNA an antagonist (17-DMAG), was assessed P....
To explore the role of triggering receptor expressed on myeloid cells 2 (TREM-2) in Pseudomonas aeruginosa (PA) keratitis.
Macrophages are the predominant innate immune cells recruited to tissues following injury or infection. These early-responding, pro-inflammatory macrophages play an essential role in amplification of inflammation. However, macrophage gene expression should be tightly regulated avert host tissue damage. In this study, we identify Kruppel-like transcription factor 6 (KLF6)-B cell leukemia/lymphoma (BCL6) signaling axis as a novel regulator inflammatory and function. Utilizing complementary...
High mobility group box 1 (HMGB1) contributes to poor disease outcome in Pseudomonas aeruginosa keratitis. This study tests the prophylactic effect of treatment with HMGB1 inhibitors, glycyrrhizin (GLY) and its derivative, carbenoxolone (CBX), for keratitis.We treated C57BL/6 (B6) mice subconjunctivally GLY or CBX, infected a noncytotoxic clinical isolate (KEI 1025) cytotoxic strain (ATCC 19660) P. aeruginosa, injected intraperitoneally either agent. Clinical score, photography slit lamp,...
The miR-183/96/182 cluster (miR-183C) is required for normal functions of sensory neurons (SN) and various immune cells, including myeloid cells (MC). This research aims to reveal the roles miR-183C SN in interplay corneal nerves (CSN) MC during Pseudomonas aeruginosa (PA) keratitis. Double-tracing mice with SN-specific (SNS) conditional knockout (CKO) age-and sex-matched wild type (WT) controls were used. Their CSN are labeled Red Fluorescent Protein (RFP); Enhanced Green (EG)FP. left...
Abstract The role of macrophages in Pseudomonas aeruginosa corneal infection susceptible (cornea perforates), C57BL/6 (B6) vs resistant heals), BALB/c mice was tested by depleting using subconjunctival injections clodronate-containing liposomes before infection. Both groups inbred treated with clodronate-liposomes compared PBS-liposomes (controls) exhibited more severe disease. In B6 mice, the cornea perforated and eye became extremely shrunken, whereas rather than healed. myeloperoxidase...
Abstract Pseudomonas aeruginosa keratitis destroys the cornea in susceptible Th1 responder C57BL/6 (B6), but not resistant Th2 (BALB/c) mice. To determine whether single Ig IL-1R-related molecule (SIGIRR) played a role resistance, mRNA and protein expression levels were tested. Both constitutively expressed of two mouse groups. A disparate pattern was detected BALB/c vs B6 mice after infection. SIGIRR decreased significantly over at 1 day postinfection. Thus, injected with an anti-SIGIRR Ab...