A5046802702- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Gastroesophageal reflux and treatments
- Reliability and Maintenance Optimization
- Cancer therapeutics and mechanisms
- Immunodeficiency and Autoimmune Disorders
- RNA and protein synthesis mechanisms
- Helicobacter pylori-related gastroenterology studies
- Microbial Natural Products and Biosynthesis
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Eosinophilic Esophagitis
- Biochemical and Molecular Research
- Physics and Engineering Research Articles
- Diagnosis and treatment of tuberculosis
- Clostridium difficile and Clostridium perfringens research
- Manufacturing Process and Optimization
- Immune responses and vaccinations
- Software Reliability and Analysis Research
- Probabilistic and Robust Engineering Design
- Respiratory and Cough-Related Research
- Fungal and yeast genetics research
- Asthma and respiratory diseases
- RNA modifications and cancer
University of Zurich
2016-2025
Universität der Bundeswehr München
2023
Center for Pediatric Endocrinology Zurich
2009-2022
Bergmann und Partner Rechtsanwälte
2013-2022
Swiss National Bank
2011-2021
Neurology, Inc
2016
Marienhaus Klinikum Hetzelstift Neustadt
2016
University Hospital Heidelberg
2016
Heidelberg University
2005-2016
Airbus (Germany)
2015
Resistance to clarithromycin among isolates of Mycobacterium chelonae and M. abscessus was observed in 18 800 (2.3%) patients tested between 1990 1995. Patients whose were resistant had either disseminated disease or chronic lung disease, the recovered after monotherapy. Sequencing gene coding for 23S rRNA peptidyltransferase region revealed a point mutation involving adenine at position 2058 (38%) 2059 (62%) 20 relapse from first 13 identified. By pulsed-field gel electrophoresis random...
Twenty-six clinical isolates of Mycobacterium abscessus resistant to amikacin were identified. Most from patients with posttympanostomy tube placement otitis media or cystic fibrosis who had received aminoglycoside therapy. Isolates highly (MICs > 1024 µg/mL) amikacin, kanamycin, gentamicin, tobramycin, and neomycin (all 2-deoxystreptamine aminoglycosides) but not streptomycin. Sequencing their 16S ribosomal (r) RNA revealed that 16 (94%) 17 an A→G mutation at position 1408. In...
ABSTRACT To study the cost of chromosomal drug resistance mutations to bacteria, we investigated fitness that confer different classes antibiotics affecting bacterial protein synthesis (aminocyclitols, 2-deoxystreptamines, macrolides). We used a model system based on an in vitro competition assay with defined Mycobacterium smegmatis laboratory mutants; selected were introduced by genetic techniques address possibility compensatory ameliorate cost. found studied often had only small cost; not...
New layer wise manufacturing technologies such as Laser Additive Manufacturing (LAM) allow innovative approaches to product design. Especially for lightweight design in aircraft applications LAM offers new possibilities load-adapted structures. However, fully capture potential of guidelines and processes have be developed. A novel approach extreme is realized by incorporating structural optimization tools, bionic structures into one process. By consequently following this process designers...
ABSTRACT Chromosomally acquired streptomycin resistance is frequently due to mutations in the gene encoding ribosomal protein S12, rpsL . The presence of several rRNA operons ( rrn ) and a single most bacterial genomes prohibits isolation streptomycin-resistant mutants which mediated by 16S rrs ). Three strains were constructed this investigation: Mycobacterium smegmatis rrnB , M. 3+ carries functional operon, i.e., rrnA (comprised 16S, 23S, 5S genes) + gene; characterized two three genes;...
Two Pseudomonas sp. strains, capable of growth on chlorinated benzenes as the sole source carbon and energy, were isolated by selective enrichment from soil samples an industrial waste deposit. Strain PS12 grew monochlorobenzene, all three isomeric dichlorobenzenes, 1,2,4-trichlorobenzene (1,2,4-TCB). PS14 additionally used 1,2,4,5-tetrachlorobenzene (1,2,4,5-TeCB). During these compounds both strains released stoichiometric amounts chloride ions. The first steps catabolism 1,2,4-TCB...
Synthetic biology provides insight into natural gene-network dynamics and enables assembly of engineered transcription circuitries for production difficult-to-access therapeutic molecules. In Mycobacterium tuberculosis EthR binds to a specific operator (O(ethR)) thereby repressing ethA preventing EthA-catalyzed conversion the prodrug ethionamide, which increases resistance pathogen this last-line-of-defense treatment. We have designed synthetic mammalian gene circuit that senses EthR-O(ethR)...
Objectives: Rifampicin, a potent first-line TB drug of the rifamycin group, shows only little activity against emerging pathogen Mycobacterium abscessus. Reportedly, bacterial resistance to rifampicin is associated with polymorphisms in target gene rpoB or presence enzymes that modify and thereby inactivate rifampicin. The aim this study was investigate role MAB_0591 (arrMab)-encoded ADP-ribosyltransferase (Arr_Mab) innate high-level M.
Summary In many species of bacteria most inducible DNA repair genes are regulated by LexA homologues and dependent on RecA for induction. We have shown previously analysing the induction recA that two mechanisms gene expression following damage exist in Mycobacterium tuberculosis . Whereas one these depends classical way, other mechanism is independent both proteins occurs absence RecA. Here we investigate generality each global response to wild‐type M. a deletion strain using microarrays....
The Mycobacterium tuberculosis ahpC gene, encoding the mycobacterial orthologue of alkylhydroperoxide reductase, undergoes an unusual regulatory cycle. levels AhpC alternate between stages expression silencing in virulent strains grown as aerated cultures, secondary to a natural loss oxyR function all tubercle bacillus, and activation static bacilli by yet undefined mechanism. reasons for this unorthodox cycle controlling antioxidant factor are currently not known. In work, M. H37Rv...
Summary Molecular genetic manipulations in mycobacteria would benefit from procedures which efficiently select for double‐crossover events by homologous recombination. Here we describe a vector‐host system gene replacement mycobacteria, the utility of was investigated using functional inactivation pyrF Mycobacterium smegmatis as model. This is based on expression wild‐type rpsL coding ribosomal protein S12 streptomycin‐resistant host. Owing to absence mycobacterial origin plasmids are unable...
Summary Lipoproteins are a subgroup of secreted bacterial proteins characterized by lipidated N‐terminus, processing which is mediated the consecutive activity prolipoprotein diacylglyceryl transferase (Lgt) and lipoprotein signal peptidase (LspA). The study LspA function has been limited mainly to non‐pathogenic microorganisms. To potential role for in pathogenesis infections, we have disrupted lspA allelic replacement Mycobacterium tuberculosis , one world's most devastating pathogens....
Quantitative susceptibility testing of clinical isolates streptomycin-resistant Mycobacterium tuberculosis demonstrated that there is a close correlation between the molecular resistance mechanism and in vitro activity streptomycin: mutations rpsL were mainly associated with high-level resistance, rrs an intermediate level wild-type exhibited low-level phenotype. Investigations revealed (i) no cross-resistance to other drugs (ii) permeability barrier may contribute because was significantly...
Drug resistance in Mycobacterium tuberculosis is a global problem, with major consequences for treatment and public health systems. As the emergence spread of drug-resistant epidemics largely influenced by impact mechanism on bacterial fitness, we wished to investigate whether compensatory evolution occurs clinical isolates M. tuberculosis. By combining information from molecular epidemiology studies genetic reconstructions measurements aminoglycoside susceptibility fitness smegmatis, have...
The protective effect of antibodies (Abs) is generally attributed to neutralization or complement activation. Using Legionella pneumophila and Mycobacterium bovis bacillus Calmette–Guérin as a model, we discovered an additional mechanism Ab-mediated protection effective against intracellular pathogens that normally evade lysosomal fusion. We show Fc receptor (FcR) engagement by Abs, which can be temporally spatially separated from bacterial infection, renders the host cell nonpermissive for...
Whole-genome sequencing allows rapid detection of drug-resistant Mycobacterium tuberculosis isolates. However, the availability high-quality data linking quantitative phenotypic drug susceptibility testing (DST) and genomic have thus far been limited.