A5086397630- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Micro and Nano Robotics
- Genetic and Kidney Cyst Diseases
- Immune cells in cancer
- CRISPR and Genetic Engineering
- Protist diversity and phylogeny
- PARP inhibition in cancer therapy
- Hereditary Neurological Disorders
- Tea Polyphenols and Effects
- Plant Molecular Biology Research
- DNA Repair Mechanisms
- Immune Response and Inflammation
The University of Tokyo
2015-2023
Waseda University
2010
Kinesin motor proteins transport intracellular cargoes throughout cells by hydrolyzing ATP and moving along microtubule tracks. Intramolecular autoinhibitory interactions have been shown for several kinesins in vitro; however, the physiological significance of autoinhibition remains poorly understood. Here, we identified four mutations stalk region domain synaptic vesicle (SV) kinesin UNC-104/KIF1A that specifically disrupt autoinhibition. These augment both cargo binding vitro. In vivo,...
Kinesin-1, the founding member of kinesin superfamily proteins, is known to use only a subset microtubules for transport in living cells. This biased proposed as guidance cue polarized neurons, but underlying mechanisms are still poorly understood. Here, we report that kinesin-1 binding changes microtubule lattice and promotes further binding. high-affinity state requires nucleotide-free state. Microtubules return initial low-affinity by washing out or non-hydrolyzable ATP analogue AMPPNP...
The kinesin-8 motor, KIF19A, accumulates at cilia tips and controls cilium length. Defective KIF19A leads to hydrocephalus female infertility because of abnormally elongated cilia. Uniquely among kinesins, possesses the dual functions motility along ciliary microtubules depolymerization microtubules. To elucidate molecular mechanisms these we solved crystal structure its motor domain determined cryo-electron microscopy complexed with a microtubule. features that enable function are clustered...
Epilepsy is a common neurological disease worldwide, and one of its causes genetic abnormalities. Here, we identified point mutation in KIF4A, member kinesin superfamily molecular motors, patients with disorders such as epilepsy, developmental delay, intellectual disability. KIF4 involved the poly (ADP-ribose) polymerase (PARP) signaling pathway, (R728Q) strengthened affinity PARP1 through elongation coiled-coil domain. Behavioral tests showed that KIF4-mutant mice exhibited mild delay lower...